Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AZKH_RS19085 Genome accession   NC_020516
Coordinates   4273505..4274638 (+) Length   377 a.a.
NCBI ID   WP_015437436.1    Uniprot ID   H0PT92
Organism   Azoarcus sp. KH32C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4268505..4279638
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AZKH_RS19055 (AZKH_3844) - 4268511..4269791 (+) 1281 WP_015437430.1 dihydroorotase -
  AZKH_RS19060 (AZKH_3845) - 4269828..4270127 (-) 300 WP_015437431.1 DUF167 family protein -
  AZKH_RS19065 (AZKH_3846) - 4270124..4270708 (-) 585 WP_015437432.1 YggT family protein -
  AZKH_RS19070 (AZKH_3847) proC 4270715..4271527 (-) 813 WP_041657589.1 pyrroline-5-carboxylate reductase -
  AZKH_RS19075 (AZKH_3848) - 4271536..4272237 (-) 702 WP_041656400.1 YggS family pyridoxal phosphate-dependent enzyme -
  AZKH_RS19080 (AZKH_3849) pilT 4272342..4273382 (+) 1041 WP_015437435.1 type IV pilus twitching motility protein PilT Machinery gene
  AZKH_RS19085 (AZKH_3850) pilU 4273505..4274638 (+) 1134 WP_015437436.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AZKH_RS19090 (AZKH_3851) - 4274745..4275905 (-) 1161 WP_015437437.1 class I SAM-dependent RNA methyltransferase -
  AZKH_RS19095 (AZKH_3852) - 4275932..4276342 (-) 411 WP_015437438.1 CopD family protein -
  AZKH_RS19100 (AZKH_3853) - 4276430..4277320 (-) 891 WP_041656401.1 SDR family oxidoreductase -
  AZKH_RS19105 (AZKH_3854) - 4277342..4278367 (+) 1026 WP_041656403.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -
  AZKH_RS19110 (AZKH_3855) - 4278372..4279508 (+) 1137 WP_015437441.1 EAL and HDOD domain-containing protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42100.43 Da        Isoelectric Point: 6.3490

>NTDB_id=56723 AZKH_RS19085 WP_015437436.1 4273505..4274638(+) (pilU) [Azoarcus sp. KH32C]
MERDQALKFMHDLLKLMVQKNGSDLFITAGFPPAIKVDGKVVPQSNQTLTGQHTAELARAVMNDRQAAEFESTKECNFAI
SPAGIGRFRANAFIQQGKVGLVLRTIAQKIPTFDELGMPTVLRDVAMAKRGLVIFVGGTGTGKTTSLAAMVDFRNEHSYG
HIITVEDPIEYVHQHKNCIVTQREIGIDTEDWEAALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQALDRIINFFPEDRRQQLLMDLSLNLRALISQRLLPLKGRKGRVPAVEVLLNSPLISDLIFKGEVPGIKEVMKRSRELG
MQTFDQSLFDLYEEELIAYEDALRNADSVNDLRLQIKLNSKHGDKDLVSGIQHLDIV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=56723 AZKH_RS19085 WP_015437436.1 4273505..4274638(+) (pilU) [Azoarcus sp. KH32C]
ATGGAACGCGATCAGGCCCTCAAGTTCATGCACGATCTGCTCAAGCTGATGGTGCAGAAGAACGGCTCCGACCTTTTCAT
CACCGCCGGCTTCCCGCCGGCCATCAAGGTCGACGGCAAAGTCGTGCCGCAGTCGAACCAGACGCTGACCGGGCAACACA
CCGCGGAACTCGCTCGCGCCGTGATGAACGACCGCCAGGCGGCGGAGTTCGAATCGACCAAGGAATGCAATTTCGCCATC
TCGCCGGCCGGCATCGGCCGCTTCCGTGCCAACGCCTTCATCCAGCAGGGCAAGGTCGGCCTCGTGCTGCGGACCATCGC
GCAAAAGATCCCGACCTTCGACGAACTCGGCATGCCGACGGTGCTGCGCGACGTCGCGATGGCCAAGCGCGGACTCGTGA
TCTTCGTCGGCGGTACCGGCACCGGTAAGACAACCTCGCTCGCCGCGATGGTCGACTTCCGCAACGAACACTCCTACGGC
CACATCATCACGGTCGAAGACCCGATCGAATACGTGCATCAGCACAAGAACTGCATCGTCACGCAGCGCGAGATCGGCAT
CGATACCGAGGACTGGGAGGCGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGTGAAATCCGCG
ACCGCGAGACGATGGACTACGCCATCGCCTTCGCCGAAACCGGCCACCTCTGCCTCGCGACGCTGCACGCCAACAGTGCC
AACCAGGCGCTCGACCGGATCATCAACTTCTTCCCCGAAGACCGCCGCCAGCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCGCGCTGATCTCGCAGCGCCTGCTGCCGCTCAAGGGCCGCAAGGGACGCGTGCCGGCAGTCGAAGTGCTGCTCAACT
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAAGTGCCAGGCATCAAGGAAGTGATGAAGCGCTCGCGCGAACTCGGC
ATGCAGACCTTCGACCAGAGCCTCTTCGACCTCTACGAGGAAGAACTGATCGCCTACGAGGACGCACTGCGCAACGCCGA
CTCCGTGAACGACCTGCGCCTGCAGATCAAGCTCAACAGCAAGCACGGCGACAAGGACCTGGTGTCCGGCATCCAGCACC
TCGACATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB H0PT92

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.088

96.552

0.599
  pilU Acinetobacter baylyi ADP1

58.924

93.634

0.552
  pilU Vibrio cholerae strain A1552

54.722

95.491

0.523
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.582

88.859

0.387
  pilT Pseudomonas aeruginosa PAK

42.09

88.859

0.374
  pilT Legionella pneumophila strain Lp02

41.298

89.92

0.371
  pilT Legionella pneumophila strain ERS1305867

41.298

89.92

0.371
  pilT Pseudomonas stutzeri DSM 10701

41.493

88.859

0.369
  pilT Acinetobacter baylyi ADP1

42.638

86.472

0.369
  pilT Acinetobacter nosocomialis M2

41.692

87.798

0.366
  pilT Acinetobacter baumannii D1279779

41.692

87.798

0.366
  pilT Acinetobacter baumannii strain A118

41.692

87.798

0.366

Multiple sequence alignment