Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C427_RS03490 Genome accession   NC_020514
Coordinates   787642..788691 (+) Length   349 a.a.
NCBI ID   WP_007640664.1    Uniprot ID   K7AEC5
Organism   Paraglaciecola psychrophila 170     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 782642..793691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C427_RS03465 (C427_0847) - 783620..784915 (-) 1296 WP_007640656.1 PotD/PotF family extracellular solute-binding protein -
  C427_RS03470 (C427_0848) - 785037..785489 (-) 453 WP_007640657.1 DUF4426 domain-containing protein -
  C427_RS03475 (C427_0849) - 785476..786018 (-) 543 WP_007640658.1 YggT family protein -
  C427_RS03480 (C427_0850) proC 786094..786915 (-) 822 WP_007640660.1 pyrroline-5-carboxylate reductase -
  C427_RS03485 (C427_0851) - 786929..787612 (-) 684 WP_007640662.1 YggS family pyridoxal phosphate-dependent enzyme -
  C427_RS03490 (C427_0852) pilT 787642..788691 (+) 1050 WP_007640664.1 type IV pilus twitching motility protein PilT Machinery gene
  C427_RS03495 (C427_0853) pilU 788695..789807 (+) 1113 WP_007640666.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C427_RS03500 (C427_0854) - 789816..790127 (+) 312 WP_007640668.1 DUF2007 domain-containing protein -
  C427_RS03505 (C427_0855) xerD 790162..791076 (+) 915 WP_007640670.1 site-specific tyrosine recombinase XerD -
  C427_RS03510 (C427_0856) dsbC 791548..792279 (+) 732 WP_015430416.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38564.37 Da        Isoelectric Point: 6.7809

>NTDB_id=56665 C427_RS03490 WP_007640664.1 787642..788691(+) (pilT) [Paraglaciecola psychrophila 170]
MDITELLAFSVKNKASDLHLSAGLPPIIRVDGEMRKLNIPALSHKEVHALIFEIMNDKQRKEYEENLETDFSFEVKDLSR
FRVNAFVQNRGAAAVLRTIPSEILSLDDLGAPQIFKEIINQPTGIILVTGATGSGKSTTLAAMVDHINSTKREHILTIED
PIEFVHENKLCVLNQREVHRDTHSFNNALRSALREDPDIILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRL
IDVFPAAEKSMIRSMLSESLRAVISQTLLKKVGGGRVAAHEIMLGIPAIRNLIREDKVPQMYSVIQTGQSHGMQTMDQCL
QRLVAQGLITAQDAAAKSIDKKPTSSFGN

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=56665 C427_RS03490 WP_007640664.1 787642..788691(+) (pilT) [Paraglaciecola psychrophila 170]
TTGGATATTACCGAACTTTTAGCCTTTAGTGTTAAAAATAAAGCTTCTGATTTACACCTTTCTGCTGGATTACCGCCAAT
CATACGTGTTGACGGTGAAATGCGAAAATTAAATATACCTGCACTCAGCCATAAAGAAGTGCATGCCCTGATTTTCGAAA
TCATGAACGACAAACAGCGTAAGGAATATGAAGAGAATCTCGAAACCGATTTTTCCTTTGAAGTAAAAGATTTATCACGC
TTTAGGGTGAATGCTTTTGTGCAAAACCGTGGTGCGGCAGCAGTATTAAGAACTATACCAAGTGAGATCTTAAGTTTAGA
TGACTTAGGTGCGCCGCAAATATTTAAAGAAATTATCAATCAGCCCACTGGCATTATACTGGTGACTGGCGCTACAGGTT
CCGGTAAAAGTACCACTTTGGCAGCCATGGTTGATCACATTAACTCAACTAAACGTGAACATATTCTAACCATTGAAGAT
CCTATCGAATTTGTCCACGAAAATAAACTTTGTGTGCTCAATCAACGTGAAGTGCATCGAGACACCCACAGCTTCAATAA
TGCTCTTCGCTCTGCGTTACGTGAAGATCCTGACATAATATTGGTAGGTGAATTACGAGATTTAGAAACGATTCGCTTAG
CTATCTCAGCCGCCGAAACCGGACATTTGGTATTTGGGACGTTACACACCAACTCGGCCCCTAAAACTATTGATAGATTA
ATCGACGTGTTTCCTGCGGCGGAAAAATCCATGATCCGTTCTATGCTGTCTGAATCGTTGCGAGCGGTTATTTCTCAAAC
CTTATTGAAAAAAGTGGGCGGGGGTAGGGTGGCAGCACACGAAATTATGCTAGGTATTCCGGCTATCCGAAACCTGATCC
GCGAAGATAAAGTGCCACAAATGTATTCGGTTATCCAAACGGGTCAATCTCATGGCATGCAAACCATGGATCAATGTTTG
CAGAGGTTAGTCGCCCAAGGCCTCATTACGGCTCAAGATGCCGCTGCAAAATCAATTGATAAAAAACCGACTTCTAGTTT
TGGGAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K7AEC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

74.493

98.854

0.736
  pilT Acinetobacter baumannii strain A118

74.493

98.854

0.736
  pilT Acinetobacter nosocomialis M2

74.203

98.854

0.734
  pilT Pseudomonas stutzeri DSM 10701

74.052

98.281

0.728
  pilT Acinetobacter baylyi ADP1

72.754

98.854

0.719
  pilT Pseudomonas aeruginosa PAK

73.669

96.848

0.713
  pilT Legionella pneumophila strain Lp02

71.848

97.708

0.702
  pilT Legionella pneumophila strain ERS1305867

71.848

97.708

0.702
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.92

96.562

0.685
  pilT Vibrio cholerae strain A1552

70.92

96.562

0.685
  pilT Neisseria gonorrhoeae MS11

64.368

99.713

0.642
  pilT Neisseria meningitidis 8013

64.368

99.713

0.642
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.143

100

0.513
  pilU Acinetobacter baylyi ADP1

42.262

96.275

0.407
  pilU Vibrio cholerae strain A1552

42.09

95.989

0.404
  pilU Pseudomonas stutzeri DSM 10701

40.597

95.989

0.39

Multiple sequence alignment