Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C427_RS03495 Genome accession   NC_020514
Coordinates   788695..789807 (+) Length   370 a.a.
NCBI ID   WP_007640666.1    Uniprot ID   K6Z1S3
Organism   Paraglaciecola psychrophila 170     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 783695..794807
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C427_RS03470 (C427_0848) - 785037..785489 (-) 453 WP_007640657.1 DUF4426 domain-containing protein -
  C427_RS03475 (C427_0849) - 785476..786018 (-) 543 WP_007640658.1 YggT family protein -
  C427_RS03480 (C427_0850) proC 786094..786915 (-) 822 WP_007640660.1 pyrroline-5-carboxylate reductase -
  C427_RS03485 (C427_0851) - 786929..787612 (-) 684 WP_007640662.1 YggS family pyridoxal phosphate-dependent enzyme -
  C427_RS03490 (C427_0852) pilT 787642..788691 (+) 1050 WP_007640664.1 type IV pilus twitching motility protein PilT Machinery gene
  C427_RS03495 (C427_0853) pilU 788695..789807 (+) 1113 WP_007640666.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C427_RS03500 (C427_0854) - 789816..790127 (+) 312 WP_007640668.1 DUF2007 domain-containing protein -
  C427_RS03505 (C427_0855) xerD 790162..791076 (+) 915 WP_007640670.1 site-specific tyrosine recombinase XerD -
  C427_RS03510 (C427_0856) dsbC 791548..792279 (+) 732 WP_015430416.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  C427_RS03515 (C427_0857) recJ 792365..794083 (+) 1719 WP_034899858.1 single-stranded-DNA-specific exonuclease RecJ -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40733.74 Da        Isoelectric Point: 5.3984

>NTDB_id=56666 C427_RS03495 WP_007640666.1 788695..789807(+) (pilU) [Paraglaciecola psychrophila 170]
MELTPYLEEMQGLGASDLFVTVGFPVSAKVNGQMTPIGGSNLSQEDALALVHDAMTEKQKLEFEETKECNFAIARDEIGR
FRCSAFWQRDMAGMVVRRIVTQIPQVSDLGLPEILKDVIMSKRGLLLFVGATGTGKSTSLAALIGHRNQHSKGHILTIED
PIEFVHQHANCVVTQREVGIDTKSFDDALKSSLRQAPDVIMIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERI
MHLAPSDQHEKLRFDLSLNMRAIIAQQLVPTPDGKGRVAAIEILLNSPLVTDLIQRNEIGGLKEAMKKGKEIGMQSFDMA
LYDLYKAGKVTLDHAIHHADSPNDLRLMIKLDSNEGNQLGSLSDVSIDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=56666 C427_RS03495 WP_007640666.1 788695..789807(+) (pilU) [Paraglaciecola psychrophila 170]
ATGGAACTCACACCTTATTTAGAAGAGATGCAAGGTTTAGGTGCATCAGATTTATTTGTCACTGTTGGCTTCCCAGTTAG
TGCTAAAGTAAACGGTCAAATGACACCTATCGGTGGCAGTAATTTAAGCCAAGAAGACGCTTTAGCCCTAGTACATGATG
CGATGACCGAAAAACAAAAACTTGAATTTGAAGAAACCAAAGAATGTAACTTTGCTATTGCTAGAGATGAAATAGGGCGT
TTTCGTTGCAGTGCTTTTTGGCAGAGAGATATGGCAGGTATGGTTGTAAGGCGTATTGTCACCCAAATACCTCAAGTTTC
AGACCTAGGTTTACCCGAAATATTAAAAGATGTGATTATGTCGAAGCGTGGCTTGTTGTTATTTGTCGGTGCCACAGGTA
CAGGTAAATCAACTTCACTAGCGGCATTGATAGGGCATAGAAATCAACACTCAAAAGGGCATATTCTGACTATAGAAGAC
CCTATAGAATTTGTACATCAACATGCCAACTGTGTGGTCACTCAGCGAGAAGTGGGTATTGATACTAAGTCTTTCGACGA
TGCATTGAAAAGTTCATTACGCCAAGCGCCTGATGTGATTATGATTGGTGAAATTCGCTCGATGGAAACCATGGAATATG
CCATGTCTTTTGCTGATACTGGGCATTTATGTGTGGCCACATTGCATGCCAATAACGCCAATCAAGCTATAGAGCGTATT
ATGCATTTGGCACCATCGGATCAACATGAAAAGCTACGCTTTGACTTGAGTTTGAATATGCGAGCCATTATTGCTCAGCA
ACTGGTTCCCACTCCAGATGGTAAAGGCCGAGTTGCAGCGATTGAAATATTGCTTAACTCTCCATTAGTCACAGATCTTA
TTCAGCGTAACGAAATTGGTGGTTTGAAAGAAGCGATGAAAAAAGGCAAGGAAATAGGTATGCAGAGTTTTGATATGGCC
TTATATGACTTGTACAAAGCTGGCAAAGTTACTCTGGATCATGCTATCCACCATGCTGACTCGCCAAACGATTTGCGCCT
TATGATCAAACTGGATTCGAATGAAGGCAATCAACTTGGATCGCTGTCTGATGTTTCTATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K6Z1S3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.165

98.378

0.592
  pilU Acinetobacter baylyi ADP1

58.217

97.027

0.565
  pilU Vibrio cholerae strain A1552

56.486

100

0.565
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.072

89.73

0.386
  pilT Pseudomonas aeruginosa PAK

38.841

93.243

0.362
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362

Multiple sequence alignment