Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KHX94_RS04190 Genome accession   NZ_CP074572
Coordinates   880042..881079 (-) Length   345 a.a.
NCBI ID   WP_213682489.1    Uniprot ID   -
Organism   Shewanella dokdonensis strain DSM 23626     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 875042..886079
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHX94_RS04155 (KHX94_04150) - 875212..875517 (+) 306 WP_213682482.1 hypothetical protein -
  KHX94_RS04160 (KHX94_04155) yciH 875708..876037 (+) 330 WP_213682483.1 stress response translation initiation inhibitor YciH -
  KHX94_RS04165 (KHX94_04160) - 876063..876623 (+) 561 WP_213682484.1 YqgE/AlgH family protein -
  KHX94_RS04170 (KHX94_04165) ruvX 876620..877063 (+) 444 WP_213682485.1 Holliday junction resolvase RuvX -
  KHX94_RS04175 (KHX94_04170) - 877073..877633 (-) 561 WP_213682486.1 glutathione peroxidase -
  KHX94_RS04180 (KHX94_04175) - 877700..878797 (-) 1098 WP_213682487.1 hypothetical protein -
  KHX94_RS04185 (KHX94_04180) pilU 878920..880032 (-) 1113 WP_213682488.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KHX94_RS04190 (KHX94_04185) pilT 880042..881079 (-) 1038 WP_213682489.1 type IV pilus twitching motility protein PilT Machinery gene
  KHX94_RS04195 (KHX94_04190) - 881117..881823 (+) 707 Protein_849 YggS family pyridoxal phosphate-dependent enzyme -
  KHX94_RS04200 (KHX94_04195) proC 881919..882743 (+) 825 WP_213682490.1 pyrroline-5-carboxylate reductase -
  KHX94_RS04205 (KHX94_04200) - 882769..883317 (+) 549 WP_213682491.1 YggT family protein -
  KHX94_RS04210 (KHX94_04205) yggU 883317..883604 (+) 288 WP_213682492.1 DUF167 family protein YggU -
  KHX94_RS04215 (KHX94_04210) - 883687..884118 (+) 432 WP_213682493.1 DUF4426 domain-containing protein -
  KHX94_RS04220 (KHX94_04215) rdgB 884153..884788 (+) 636 WP_244859322.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  KHX94_RS04225 (KHX94_04220) hemW 884788..885921 (+) 1134 WP_213682494.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38357.03 Da        Isoelectric Point: 6.9483

>NTDB_id=565348 KHX94_RS04190 WP_213682489.1 880042..881079(-) (pilT) [Shewanella dokdonensis strain DSM 23626]
MEITELLAFSVKHNASDLHLSSGVPPLIRVDGEVRKLNVPELTHQDVHRLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQARGAAAVFRTIPSDILTLEHLGAPEIFKKISSYPRGLVLVTGPTGSGKSTTLAAMIDYVNDNRHDHILTIED
PIEFVHKPKNCLINQREVHRHTLSFAAALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPSGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQSL
QHLVARGVITREDALSKSSNKQLNL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=565348 KHX94_RS04190 WP_213682489.1 880042..881079(-) (pilT) [Shewanella dokdonensis strain DSM 23626]
ATGGAAATCACTGAATTATTGGCTTTTAGTGTAAAGCATAACGCCTCGGATCTGCATCTGTCTTCCGGGGTGCCACCGTT
GATCCGCGTTGATGGGGAGGTGAGAAAACTCAATGTCCCAGAATTGACGCATCAGGATGTGCATCGGCTGGTGTATGACA
TCATGAACGACAAACAGCGTAAGGATTATGAAGAGCACCTGGAAATCGATTTTTCCTTTGAGGTGCCCAATCTGGCGCGG
TTCAGGGTGAACGCCTTTAATCAGGCCCGTGGCGCGGCCGCTGTCTTTCGCACCATTCCGAGCGATATTCTAACGCTGGA
GCATCTGGGCGCTCCTGAAATTTTTAAGAAAATCTCCTCATATCCTCGTGGATTAGTGTTGGTTACCGGGCCTACAGGTT
CTGGTAAAAGTACGACCTTGGCAGCCATGATTGATTATGTGAATGACAATCGTCACGACCATATTCTCACCATTGAAGAC
CCCATCGAATTTGTACACAAGCCTAAAAACTGTTTGATCAATCAGCGTGAAGTCCATCGCCATACATTGAGCTTTGCGGC
GGCGCTGCGTAGTGCTTTGCGTGAAGACCCGGATGTCATCCTAGTGGGTGAAATGCGTGACCTGGAAACCATCCGCTTAG
CATTAACCGCGGCTGAAACTGGTCATCTGGTGTTTGGTACCTTGCATACCACCTCAGCCGCTAAAACCATTGACCGTGTG
GTTGACGTGTTCCCATCGGGTGAAAAAGATATGGTGCGCACCATGTTGTCTGAATCCTTGCAGGCGGTAATTTCACAGAC
CTTGATCAAGAAAATCGGTGGTGGCCGGGTCGCGGCTCACGAGATCATGATGGGGACGCCCGCTATCCGTAACCTCATTC
GTGAGGATAAAGTGGCGCAGATGTATTCCGCCATTCAGACGGGGATGGCGCACGGCATGCAGACACTGGAGCAGAGCCTG
CAACATCTGGTGGCTCGTGGGGTGATCACCCGTGAAGATGCGCTATCTAAGAGTTCTAACAAACAACTAAATCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

80.117

99.13

0.794
  pilT Acinetobacter baumannii strain A118

80.117

99.13

0.794
  pilT Acinetobacter nosocomialis M2

79.825

99.13

0.791
  pilT Pseudomonas aeruginosa PAK

78.698

97.971

0.771
  pilT Acinetobacter baylyi ADP1

80.243

95.362

0.765
  pilT Pseudomonas stutzeri DSM 10701

76.608

99.13

0.759
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.074

97.681

0.733
  pilT Vibrio cholerae strain A1552

75.074

97.681

0.733
  pilT Legionella pneumophila strain Lp02

72.012

99.42

0.716
  pilT Legionella pneumophila strain ERS1305867

72.012

99.42

0.716
  pilT Neisseria meningitidis 8013

68.513

99.42

0.681
  pilT Neisseria gonorrhoeae MS11

68.222

99.42

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.261

0.493
  pilU Pseudomonas stutzeri DSM 10701

42.651

100

0.429
  pilU Vibrio cholerae strain A1552

41.499

100

0.417
  pilU Acinetobacter baylyi ADP1

39.169

97.681

0.383