Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KHX94_RS04185 Genome accession   NZ_CP074572
Coordinates   878920..880032 (-) Length   370 a.a.
NCBI ID   WP_213682488.1    Uniprot ID   -
Organism   Shewanella dokdonensis strain DSM 23626     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 873920..885032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHX94_RS04150 (KHX94_04145) - 874461..875021 (-) 561 WP_213682481.1 DUF4136 domain-containing protein -
  KHX94_RS04155 (KHX94_04150) - 875212..875517 (+) 306 WP_213682482.1 hypothetical protein -
  KHX94_RS04160 (KHX94_04155) yciH 875708..876037 (+) 330 WP_213682483.1 stress response translation initiation inhibitor YciH -
  KHX94_RS04165 (KHX94_04160) - 876063..876623 (+) 561 WP_213682484.1 YqgE/AlgH family protein -
  KHX94_RS04170 (KHX94_04165) ruvX 876620..877063 (+) 444 WP_213682485.1 Holliday junction resolvase RuvX -
  KHX94_RS04175 (KHX94_04170) - 877073..877633 (-) 561 WP_213682486.1 glutathione peroxidase -
  KHX94_RS04180 (KHX94_04175) - 877700..878797 (-) 1098 WP_213682487.1 hypothetical protein -
  KHX94_RS04185 (KHX94_04180) pilU 878920..880032 (-) 1113 WP_213682488.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KHX94_RS04190 (KHX94_04185) pilT 880042..881079 (-) 1038 WP_213682489.1 type IV pilus twitching motility protein PilT Machinery gene
  KHX94_RS04195 (KHX94_04190) - 881117..881823 (+) 707 Protein_849 YggS family pyridoxal phosphate-dependent enzyme -
  KHX94_RS04200 (KHX94_04195) proC 881919..882743 (+) 825 WP_213682490.1 pyrroline-5-carboxylate reductase -
  KHX94_RS04205 (KHX94_04200) - 882769..883317 (+) 549 WP_213682491.1 YggT family protein -
  KHX94_RS04210 (KHX94_04205) yggU 883317..883604 (+) 288 WP_213682492.1 DUF167 family protein YggU -
  KHX94_RS04215 (KHX94_04210) - 883687..884118 (+) 432 WP_213682493.1 DUF4426 domain-containing protein -
  KHX94_RS04220 (KHX94_04215) rdgB 884153..884788 (+) 636 WP_244859322.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40983.04 Da        Isoelectric Point: 6.1877

>NTDB_id=565347 KHX94_RS04185 WP_213682488.1 878920..880032(-) (pilU) [Shewanella dokdonensis strain DSM 23626]
MDVRPFLEEMVARKASDLFVTAGFPPSAKVDGELRPLAENPFNQEQALEFVESLMTPENKKAFHDSKECNFAIGVKGLGR
FRVSAFWQREAAGCVMRRIEIRIPTMEELSLPAILRDLVMSKRGLVIMVGGTGTGKSTSLAALIGYRNENSKGHILTIED
PIEFVHQHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHRQLLFDLSLNLRGIVAQQLIPKADGSGRRAAIEILINTPRVASIIQNNELHLLKETMAKSNEQGMQTFDQA
LFKLFNDGEITYAEALRHADSPNDLRLMIKLQSGTTGSAGSMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=565347 KHX94_RS04185 WP_213682488.1 878920..880032(-) (pilU) [Shewanella dokdonensis strain DSM 23626]
ATGGACGTCAGACCTTTTCTTGAGGAAATGGTAGCGCGTAAAGCCTCCGACCTGTTTGTAACGGCGGGGTTCCCGCCAAG
CGCCAAGGTAGATGGGGAGCTGCGGCCATTAGCCGAGAATCCGTTTAACCAAGAGCAGGCGCTGGAATTTGTAGAGTCAT
TGATGACGCCGGAAAACAAAAAGGCGTTTCATGACAGCAAGGAATGTAATTTTGCGATTGGCGTTAAAGGCTTAGGGCGC
TTCCGTGTCAGCGCTTTCTGGCAGCGAGAAGCCGCAGGCTGTGTAATGCGGCGCATTGAAATCCGTATCCCCACCATGGA
AGAACTCAGTTTACCCGCCATTCTCAGAGATCTGGTGATGAGTAAACGGGGGCTGGTGATCATGGTCGGTGGTACTGGTA
CGGGTAAATCTACCTCGCTGGCAGCACTGATTGGCTACCGTAATGAAAATTCCAAAGGCCATATCCTGACTATTGAAGAC
CCCATCGAATTTGTGCATCAGCATCGCAAGAGCATTATTACCCAGCGCGAAGTTGGCATTGATACCGAATCTTTTGATGC
GGCACTGAAAAGTTCGTTGCGACAGGCGCCAGACGTGATTCTTATTGGCGAAATCCGTAGTCAGGAAACCATGGAGTTTG
CACTATCTTTCGCTGAAACCGGCCACCTGTGTATGGCTACCTTGCACGCCAATAATGCCAACCAGGCGCTGGATCGCATA
ATGCACTTGGTGCCGGAAAGTAAGCATCGGCAATTGTTATTTGATTTGTCTTTGAATTTACGTGGCATTGTGGCGCAGCA
ATTGATCCCCAAGGCGGATGGTAGCGGCCGCCGCGCCGCCATTGAAATCCTCATCAACACGCCTCGCGTTGCCAGTATCA
TTCAAAACAATGAGTTACATCTGCTGAAAGAAACTATGGCGAAATCGAATGAGCAGGGTATGCAAACCTTCGACCAGGCG
TTGTTTAAGTTATTTAATGATGGCGAGATCACCTATGCCGAAGCCTTACGCCATGCCGATTCGCCCAACGATTTGCGCCT
GATGATCAAACTGCAAAGTGGCACGACTGGGTCGGCGGGTTCCATGGAAGGTGTGACGCTGGATATGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.108

100

0.603
  pilU Vibrio cholerae strain A1552

56.757

100

0.568
  pilU Acinetobacter baylyi ADP1

57.67

95.135

0.549
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.111

97.297

0.4
  pilT Acinetobacter baumannii strain A118

40.351

92.432

0.373
  pilT Acinetobacter nosocomialis M2

40.351

92.432

0.373
  pilT Acinetobacter baumannii D1279779

40.351

92.432

0.373
  pilT Legionella pneumophila strain Lp02

41.39

89.459

0.37
  pilT Legionella pneumophila strain ERS1305867

41.39

89.459

0.37
  pilT Pseudomonas aeruginosa PAK

39.71

93.243

0.37
  pilT Acinetobacter baylyi ADP1

40.058

92.432

0.37
  pilT Vibrio cholerae strain A1552

40.419

90.27

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.27

0.365
  pilT Pseudomonas stutzeri DSM 10701

38.841

93.243

0.362
  pilT Neisseria gonorrhoeae MS11

40.854

88.649

0.362
  pilT Neisseria meningitidis 8013

40.854

88.649

0.362