Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KF947_RS13480 Genome accession   NZ_CP074200
Coordinates   2921426..2922469 (+) Length   347 a.a.
NCBI ID   WP_227404184.1    Uniprot ID   -
Organism   Halomonas sp. FeN2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2916426..2927469
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KF947_RS13450 (KF947_13420) - 2916766..2917215 (-) 450 WP_227404180.1 DUF4426 domain-containing protein -
  KF947_RS13455 (KF947_13425) metW 2917257..2917850 (-) 594 WP_027958266.1 methionine biosynthesis protein MetW -
  KF947_RS13460 (KF947_13430) metX 2917850..2919076 (-) 1227 WP_227404181.1 homoserine O-succinyltransferase MetX -
  KF947_RS13465 (KF947_13435) - 2919146..2919739 (-) 594 WP_133731570.1 YggT family protein -
  KF947_RS13470 (KF947_13440) proC 2919797..2920621 (-) 825 WP_227404182.1 pyrroline-5-carboxylate reductase -
  KF947_RS13475 (KF947_13445) - 2920677..2921393 (-) 717 WP_227404183.1 YggS family pyridoxal phosphate-dependent enzyme -
  KF947_RS13480 (KF947_13450) pilT 2921426..2922469 (+) 1044 WP_227404184.1 type IV pilus twitching motility protein PilT Machinery gene
  KF947_RS13485 (KF947_13455) pilU 2922462..2923643 (+) 1182 WP_227404185.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KF947_RS13490 (KF947_13460) - 2923695..2924834 (-) 1140 WP_227404186.1 anhydro-N-acetylmuramic acid kinase -
  KF947_RS13495 (KF947_13465) tyrS 2924969..2926168 (+) 1200 WP_035582992.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38174.65 Da        Isoelectric Point: 6.0078

>NTDB_id=563828 KF947_RS13480 WP_227404184.1 2921426..2922469(+) (pilT) [Halomonas sp. FeN2]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAIDNNDVRKLIYDIMNDRQRRDYEEHLEIDFSFEVPGIAR
FRVNAFTQARGAGAVFRTIPNQVLSMQTLGLGEVFERLAMLPRGLVLVTGPTGSGKSTTLAAMIDYINDHRYEHILTIED
PVEFVHASKRCLINQREVHRDTHSFADALRSALREDPDVILVGELRDLETIRLALTAAETGHLVLGTLHTTSAAKTIDRI
IDVFPGDEKAMVRSMLSESLQAVVSQSLLKRQGGGRVAAHEILIATSAVRNLIREDKVAQMYSAIQTGGSLGMQTLDSAL
SRLVKEGTVSLEEAQLSAKGTLAIKDE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=563828 KF947_RS13480 WP_227404184.1 2921426..2922469(+) (pilT) [Halomonas sp. FeN2]
ATGGATATTACCGAACTGCTAGCATTCTCGGCAAAGCAGAATGCCTCTGACTTGCACCTTTCGGCCGGTTTGCCGCCCAT
GATACGTGTTGATGGTGACATTCGTCGGCTCAATGTGCCCGCTATCGATAATAATGATGTGCGCAAATTGATCTACGACA
TCATGAATGATCGTCAGCGGCGCGACTACGAAGAGCACCTGGAAATTGATTTTTCCTTTGAAGTGCCGGGGATCGCCCGC
TTCCGCGTTAACGCCTTTACGCAAGCGCGCGGGGCGGGGGCCGTGTTTCGCACCATTCCTAATCAGGTGCTCTCCATGCA
GACCCTGGGGCTCGGTGAGGTGTTCGAGCGCCTGGCGATGTTGCCGCGGGGCCTGGTGCTGGTGACTGGGCCAACGGGGT
CGGGCAAGAGCACCACGCTGGCCGCGATGATCGATTACATTAACGATCATCGTTACGAGCATATTCTCACCATTGAAGAT
CCCGTTGAATTTGTCCACGCCAGCAAGCGCTGTTTGATTAACCAACGCGAAGTGCATCGCGACACCCATAGCTTTGCTGA
TGCGCTGCGTAGCGCGCTGCGTGAAGACCCGGATGTGATCCTGGTCGGCGAGCTGCGCGATTTGGAGACCATCCGTTTGG
CGCTGACCGCTGCTGAAACCGGTCACCTGGTGCTGGGAACGCTGCATACCACTTCTGCTGCCAAGACAATCGACCGGATT
ATTGATGTTTTCCCCGGCGACGAGAAAGCCATGGTGCGCTCCATGCTGTCGGAATCGCTCCAGGCAGTGGTGTCGCAATC
GCTGCTTAAACGCCAGGGCGGCGGGCGAGTAGCGGCCCATGAGATCCTCATTGCCACTTCTGCAGTGCGCAACTTGATCC
GTGAAGACAAAGTGGCGCAGATGTACTCGGCCATTCAAACCGGCGGCAGCCTGGGCATGCAAACCCTGGATTCCGCGCTG
TCGCGGTTAGTGAAAGAAGGCACCGTAAGCTTAGAAGAGGCGCAGCTGAGTGCGAAGGGGACGCTGGCGATAAAAGATGA
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.876

97.695

0.761
  pilT Pseudomonas stutzeri DSM 10701

77.581

97.695

0.758
  pilT Acinetobacter nosocomialis M2

75.516

97.695

0.738
  pilT Acinetobacter baumannii D1279779

75.516

97.695

0.738
  pilT Acinetobacter baumannii strain A118

75.516

97.695

0.738
  pilT Acinetobacter baylyi ADP1

74.926

97.695

0.732
  pilT Legionella pneumophila strain Lp02

71.893

97.406

0.7
  pilT Legionella pneumophila strain ERS1305867

71.893

97.406

0.7
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.149

96.542

0.677
  pilT Vibrio cholerae strain A1552

70.149

96.542

0.677
  pilT Neisseria meningitidis 8013

65.205

98.559

0.643
  pilT Neisseria gonorrhoeae MS11

64.912

98.559

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.731

96.542

0.519
  pilU Pseudomonas stutzeri DSM 10701

43.068

97.695

0.421
  pilU Vibrio cholerae strain A1552

41.003

97.695

0.401
  pilU Acinetobacter baylyi ADP1

41.071

96.83

0.398