Detailed information NTG0563829

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KF947_RS13485
• Genome accession: NZ_CP074200
• Coordinates: 2922462..2923643 (+)
• Length: 393 a.a.
• NCBI ID: WP_227404185.1
• Uniprot ID: -
• Organism: Halomonas sp. FeN2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2917462..2928643

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KF947_RS13460 (KF947_13430)	metX	2917850..2919076 (-)	1227	WP_227404181.1	homoserine O-succinyltransferase MetX	-
KF947_RS13465 (KF947_13435)	-	2919146..2919739 (-)	594	WP_133731570.1	YggT family protein	-
KF947_RS13470 (KF947_13440)	proC	2919797..2920621 (-)	825	WP_227404182.1	pyrroline-5-carboxylate reductase	-
KF947_RS13475 (KF947_13445)	-	2920677..2921393 (-)	717	WP_227404183.1	YggS family pyridoxal phosphate-dependent enzyme	-
KF947_RS13480 (KF947_13450)	pilT	2921426..2922469 (+)	1044	WP_227404184.1	type IV pilus twitching motility protein PilT	Machinery gene
KF947_RS13485 (KF947_13455)	pilU	2922462..2923643 (+)	1182	WP_227404185.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KF947_RS13490 (KF947_13460)	-	2923695..2924834 (-)	1140	WP_227404186.1	anhydro-N-acetylmuramic acid kinase	-
KF947_RS13495 (KF947_13465)	tyrS	2924969..2926168 (+)	1200	WP_035582992.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 393 a.a.
• Molecular weight: 43513.65 Da
• Isoelectric Point: 6.2632

>NTDB_id=563829 KF947_RS13485 WP_227404185.1 2922462..2923643(+) (pilU) [Halomonas sp. FeN2]
MNKDSSIQAPSVELSPTQWLHELLDIMVEQDASDLLISVGAPPSLKMPDGLVPLGQQRLTPHQVNELVTHTLPEGLRERF
ANEREANFALSREGKGRFRVSAFQQRNQMAMVVRSIAIEIPRLEALGVPEQLAELAQAKRGLVLVVGGTGTGKSTTLAAM
IQQRNETVGGHIISIEDPIEYLHPHKRAIVNQREVGIDTESFEVALKNTLRQAPDVILIGEIRTRETMEHALTFAETGHL
CLATLHANNANQALERIIHFFPHERHEQIWMDLSLNLRAVVAQQLLPTLAGGRRAAIEIMLQSPRIADLIRKGEVAEIKA
AMAKSRDAGMQTFDQALHDLHQAGHISREVAMAHADSANDLRMLLNYSDAKGRNLSLDEQVPSHLGLRDGDDY

Nucleotide

• Length: 1182 bp

>NTDB_id=563829 KF947_RS13485 WP_227404185.1 2922462..2923643(+) (pilU) [Halomonas sp. FeN2]
ATGAATAAAGACAGTTCAATTCAGGCGCCGTCTGTCGAGCTTTCGCCTACTCAATGGCTGCATGAGCTGCTCGACATTAT
GGTTGAGCAGGACGCTTCCGATCTGCTTATCTCGGTAGGCGCGCCGCCAAGCCTAAAAATGCCCGATGGCCTGGTGCCGC
TGGGTCAGCAGCGTTTAACCCCCCATCAGGTTAACGAACTGGTTACCCATACGCTGCCGGAAGGGTTGCGTGAACGTTTT
GCCAATGAGCGAGAGGCGAATTTTGCCCTTAGCCGCGAGGGCAAAGGGCGTTTTCGGGTCAGTGCCTTCCAGCAGCGCAA
TCAGATGGCGATGGTGGTGCGCAGCATCGCGATTGAAATTCCTCGCTTGGAGGCGCTAGGCGTACCGGAGCAGTTGGCTG
AATTAGCACAAGCTAAGCGCGGGCTGGTACTGGTGGTGGGCGGCACGGGAACGGGTAAATCAACGACCCTGGCGGCGATG
ATTCAGCAGCGCAACGAAACCGTCGGCGGCCATATCATCAGTATCGAAGACCCGATTGAGTACCTGCACCCGCACAAGCG
TGCGATTGTGAATCAGCGCGAAGTGGGGATTGATACCGAGTCGTTTGAGGTTGCGCTTAAAAACACCCTGCGCCAGGCGC
CGGATGTGATTCTGATTGGTGAAATCCGCACTCGGGAGACCATGGAACACGCGCTGACATTTGCTGAAACCGGTCACCTC
TGTTTGGCGACACTGCATGCCAATAACGCTAACCAGGCGCTGGAGCGCATCATTCACTTCTTTCCCCATGAACGCCATGA
GCAGATATGGATGGATCTTTCGCTGAATCTGCGTGCGGTCGTCGCCCAGCAGCTGCTGCCCACGCTTGCGGGTGGGCGCC
GTGCGGCCATTGAGATCATGCTGCAGTCGCCGCGCATTGCCGACTTGATCCGTAAAGGAGAGGTCGCCGAGATTAAAGCA
GCAATGGCCAAGTCTCGCGATGCCGGGATGCAGACCTTTGATCAGGCGCTGCATGACCTGCATCAGGCGGGGCATATCAG
TAGAGAGGTGGCCATGGCCCACGCTGACTCGGCTAACGACCTGCGTATGCTGCTCAATTACAGCGACGCCAAGGGGCGTA
ACCTCTCACTAGACGAGCAGGTGCCTAGCCATCTAGGGCTGCGCGATGGCGATGATTATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (70-287)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.933	95.42	0.562
pilU	Vibrio cholerae strain A1552	56.284	93.13	0.524
pilU	Acinetobacter baylyi ADP1	55.028	91.094	0.501
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.659	92.621	0.377