Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KFF03_RS01365 Genome accession   NZ_CP073745
Coordinates   279149..280183 (+) Length   344 a.a.
NCBI ID   WP_255858484.1    Uniprot ID   A0A9J7AI66
Organism   Bacterioplanoides sp. SCSIO 12839     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 274149..285183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFF03_RS01345 (KFF03_01345) - 274482..276470 (+) 1989 WP_255858480.1 DNA topoisomerase III -
  KFF03_RS01350 (KFF03_01350) - 276613..277206 (-) 594 WP_255858481.1 YggT family protein -
  KFF03_RS01355 (KFF03_01355) proC 277280..278098 (-) 819 WP_255858482.1 pyrroline-5-carboxylate reductase -
  KFF03_RS01360 (KFF03_01360) - 278184..278870 (-) 687 WP_255858483.1 YggS family pyridoxal phosphate-dependent enzyme -
  KFF03_RS01365 (KFF03_01365) pilT 279149..280183 (+) 1035 WP_255858484.1 type IV pilus twitching motility protein PilT Machinery gene
  KFF03_RS01370 (KFF03_01370) pilU 280231..281346 (+) 1116 WP_255858485.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KFF03_RS01375 (KFF03_01375) - 281532..282758 (+) 1227 WP_255858486.1 acyl-CoA dehydrogenase family protein -
  KFF03_RS01380 (KFF03_01380) - 282840..283691 (+) 852 WP_255858487.1 SDR family oxidoreductase -
  KFF03_RS01385 (KFF03_01385) - 283688..284782 (+) 1095 WP_255858488.1 phosphotransferase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37910.31 Da        Isoelectric Point: 6.4348

>NTDB_id=560367 KFF03_RS01365 WP_255858484.1 279149..280183(+) (pilT) [Bacterioplanoides sp. SCSIO 12839]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPSLGHKEVHGLVYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMDQLGMGQVFKDLADTHKGIVLVTGPTGSGKSTTLAAMLDYINESKYYHILTVED
PIEFVHESKKSLINQREVHRDTLGFNEALRSALREDPDVILVGEMRDLETIRLALTAAETGHVVFGTLHTSSAAKTIDRI
VDVFPSEEQAMVRSMLSESLQGVISQTLLKKSGGGRVAAHEIMVGTPAIRNLIREDKVAQMYSAIQTGGAYGMTTMDQSL
QNLVQKGLITRDTAREKAKVPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=560367 KFF03_RS01365 WP_255858484.1 279149..280183(+) (pilT) [Bacterioplanoides sp. SCSIO 12839]
ATGGATATTACCGAACTGTTGGCGTTCAGCGCGAAGCAAGGCGCGTCCGACTTACACCTGTCGGCAGGCCTGCCCCCTAT
GATTCGTGTTGACGGCGATGTGCGTCGTATTAATCTGCCGTCGCTGGGCCATAAAGAAGTACACGGTCTGGTATATGACA
TCATGAACGATAAGCAGCGTAAGGATTTTGAAGAATTCCTTGAGACTGACTTCTCCTTCGAAGTGCCGGGCGTGGCGCGT
TTCCGGGTAAACGCCTTTAACCATAACCGTGGTGCTGGCGCGGTATTCCGGACCATTCCTTCCAAAGTACTGACCATGGA
CCAGCTCGGCATGGGTCAGGTGTTTAAAGACCTGGCAGATACCCATAAAGGTATTGTGCTGGTAACTGGACCAACCGGTT
CGGGTAAGTCGACCACGCTGGCGGCGATGCTGGATTACATTAACGAAAGTAAGTATTACCACATCCTGACGGTAGAAGAT
CCGATCGAATTTGTGCACGAATCGAAGAAATCCCTGATTAACCAACGGGAAGTACACCGTGACACCCTGGGCTTTAACGA
AGCGCTGCGTTCTGCCCTGCGGGAAGACCCAGATGTGATTTTGGTGGGCGAGATGCGAGACCTGGAAACCATCCGTCTGG
CGTTAACTGCAGCCGAAACCGGTCACGTGGTGTTTGGTACGTTGCATACCAGCTCGGCAGCTAAAACCATTGACCGGATT
GTTGACGTGTTCCCGTCCGAAGAACAAGCCATGGTGCGTTCGATGTTGTCTGAATCTCTGCAGGGCGTGATCTCTCAGAC
ACTGCTGAAGAAAAGTGGCGGTGGTCGTGTGGCGGCTCACGAAATCATGGTCGGTACTCCGGCGATTCGTAACCTGATTC
GTGAAGATAAAGTGGCGCAGATGTATTCTGCTATCCAGACCGGTGGTGCCTATGGCATGACCACGATGGATCAATCGTTA
CAAAATCTGGTGCAAAAAGGCCTGATTACCCGCGATACTGCCCGTGAAAAAGCTAAAGTGCCGGATAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

86.047

100

0.86
  pilT Pseudomonas stutzeri DSM 10701

84.593

100

0.846
  pilT Acinetobacter nosocomialis M2

82.849

100

0.828
  pilT Acinetobacter baumannii D1279779

82.849

100

0.828
  pilT Acinetobacter baumannii strain A118

82.849

100

0.828
  pilT Acinetobacter baylyi ADP1

79.651

100

0.797
  pilT Legionella pneumophila strain Lp02

72.781

98.256

0.715
  pilT Legionella pneumophila strain ERS1305867

72.781

98.256

0.715
  pilT Neisseria meningitidis 8013

67.941

98.837

0.672
  pilT Neisseria gonorrhoeae MS11

67.647

98.837

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.062

97.965

0.657
  pilT Vibrio cholerae strain A1552

67.062

97.965

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.333

100

0.535
  pilU Pseudomonas stutzeri DSM 10701

39.701

97.384

0.387
  pilU Acinetobacter baylyi ADP1

38.15

100

0.384
  pilU Vibrio cholerae strain A1552

40.615

94.477

0.384