Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KFF03_RS01370 Genome accession   NZ_CP073745
Coordinates   280231..281346 (+) Length   371 a.a.
NCBI ID   WP_255858485.1    Uniprot ID   -
Organism   Bacterioplanoides sp. SCSIO 12839     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 275231..286346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFF03_RS01350 (KFF03_01350) - 276613..277206 (-) 594 WP_255858481.1 YggT family protein -
  KFF03_RS01355 (KFF03_01355) proC 277280..278098 (-) 819 WP_255858482.1 pyrroline-5-carboxylate reductase -
  KFF03_RS01360 (KFF03_01360) - 278184..278870 (-) 687 WP_255858483.1 YggS family pyridoxal phosphate-dependent enzyme -
  KFF03_RS01365 (KFF03_01365) pilT 279149..280183 (+) 1035 WP_255858484.1 type IV pilus twitching motility protein PilT Machinery gene
  KFF03_RS01370 (KFF03_01370) pilU 280231..281346 (+) 1116 WP_255858485.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KFF03_RS01375 (KFF03_01375) - 281532..282758 (+) 1227 WP_255858486.1 acyl-CoA dehydrogenase family protein -
  KFF03_RS01380 (KFF03_01380) - 282840..283691 (+) 852 WP_255858487.1 SDR family oxidoreductase -
  KFF03_RS01385 (KFF03_01385) - 283688..284782 (+) 1095 WP_255858488.1 phosphotransferase family protein -
  KFF03_RS01390 (KFF03_01390) - 284784..285473 (+) 690 WP_255858489.1 histidine phosphatase family protein -
  KFF03_RS01395 (KFF03_01395) - 285503..286273 (+) 771 WP_255858490.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41109.19 Da        Isoelectric Point: 6.2877

>NTDB_id=560368 KFF03_RS01370 WP_255858485.1 280231..281346(+) (pilU) [Bacterioplanoides sp. SCSIO 12839]
MAMDKFLRLMVEKGASDLFITAGVPPSMKVHGSIMPLTKNALGAEQTRDIVLGLMNSKQQDEFEEKKELNFAINASGIGR
FRASAFYQRNVAGMVLRRIETRIPSLDQLGLPDIIKDLSMSKRGLILFVGATGAGKSTSLAAMIGYRNRNSKGHILSIED
PIEFIHQHESCIVTQREVGLDTETFEVGLKNCLRQAPDVIMIGEVRSADTMEHAITFAETGHLCLATLHSNNANQALERV
IHFFPSERHGQIWMDLSLNLKAIVAQQLIPTPDGRGRRAVIEVLINTPLASDLIRKGEVHELKPLMRKSNEVGMQTYDQA
LYALYEQGEITYEDAIAHADSPNDLRLMIKLGSETDADSLDSATSKLRLQD

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=560368 KFF03_RS01370 WP_255858485.1 280231..281346(+) (pilU) [Bacterioplanoides sp. SCSIO 12839]
ATGGCTATGGATAAGTTTTTGCGCCTGATGGTGGAAAAAGGCGCCTCAGATCTGTTTATTACAGCCGGCGTTCCACCGTC
GATGAAAGTGCATGGCAGCATTATGCCGCTGACCAAAAACGCACTGGGCGCTGAGCAAACCCGGGATATTGTTCTGGGTT
TGATGAACAGCAAACAGCAGGACGAGTTCGAAGAGAAAAAAGAACTCAACTTTGCCATCAACGCCAGTGGTATTGGCCGT
TTCAGGGCCTCCGCGTTTTATCAGCGTAATGTGGCTGGCATGGTACTGCGGCGTATCGAAACCCGGATTCCAAGTCTGGA
TCAGCTTGGCTTGCCCGACATTATCAAAGATTTGTCGATGTCTAAGCGTGGCCTGATTTTATTTGTTGGTGCCACCGGTG
CCGGTAAATCGACCTCGCTGGCGGCGATGATTGGTTATCGTAATCGCAACTCCAAAGGGCATATTCTCAGCATTGAAGAC
CCAATCGAATTTATTCACCAACACGAAAGCTGCATTGTCACGCAGCGGGAAGTGGGGCTGGATACCGAGACGTTTGAGGT
TGGCCTGAAGAACTGCCTGCGTCAGGCTCCGGATGTCATCATGATTGGTGAGGTGCGCTCGGCGGACACCATGGAGCACG
CCATTACCTTCGCTGAAACCGGTCACTTATGTCTGGCGACCTTGCACTCCAATAACGCTAACCAGGCACTGGAGCGGGTG
ATTCACTTCTTCCCGTCGGAGCGTCACGGTCAGATCTGGATGGACTTATCGCTCAACTTAAAAGCGATTGTTGCCCAGCA
ATTAATTCCGACCCCGGATGGCCGTGGCCGTCGGGCGGTAATTGAAGTGTTAATTAATACACCGCTGGCGTCGGATTTAA
TTCGTAAAGGTGAAGTGCACGAGTTAAAACCGCTGATGCGTAAATCCAACGAAGTGGGCATGCAAACCTACGATCAGGCG
TTGTACGCGTTATACGAGCAGGGTGAAATCACTTATGAAGATGCCATTGCTCATGCGGACTCACCAAACGACCTGCGTCT
GATGATTAAACTGGGCTCGGAAACCGATGCGGACTCGCTGGATAGTGCGACTTCTAAGCTAAGGTTGCAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

73.784

99.73

0.736
  pilU Acinetobacter baylyi ADP1

61.345

96.226

0.59
  pilU Vibrio cholerae strain A1552

56

94.34

0.528
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.647

91.644

0.391
  pilT Acinetobacter baumannii strain A118

39.766

92.183

0.367
  pilT Acinetobacter nosocomialis M2

39.766

92.183

0.367
  pilT Acinetobacter baumannii D1279779

39.766

92.183

0.367
  pilT Pseudomonas aeruginosa PAK

39.13

92.992

0.364