Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KDD30_RS13170 Genome accession   NZ_CP073578
Coordinates   2852629..2853654 (-) Length   341 a.a.
NCBI ID   WP_211646262.1    Uniprot ID   -
Organism   Photobacterium sp. GJ3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2847629..2858654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KDD30_RS13145 (KDD30_13145) gshB 2847749..2848699 (+) 951 WP_211646257.1 glutathione synthase -
  KDD30_RS13150 (KDD30_13150) - 2848884..2849447 (+) 564 WP_211646258.1 YqgE/AlgH family protein -
  KDD30_RS13155 (KDD30_13155) ruvX 2849444..2849872 (+) 429 WP_211646259.1 Holliday junction resolvase RuvX -
  KDD30_RS13160 (KDD30_13160) - 2849994..2851304 (-) 1311 WP_211646260.1 YjiH family protein -
  KDD30_RS13165 (KDD30_13165) pilU 2851496..2852599 (-) 1104 WP_211646261.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KDD30_RS13170 (KDD30_13170) pilT 2852629..2853654 (-) 1026 WP_211646262.1 type IV pilus twitching motility protein PilT Machinery gene
  KDD30_RS13175 (KDD30_13175) - 2853677..2854378 (+) 702 WP_211646263.1 YggS family pyridoxal phosphate-dependent enzyme -
  KDD30_RS13180 (KDD30_13180) proC 2854427..2855245 (+) 819 WP_211646264.1 pyrroline-5-carboxylate reductase -
  KDD30_RS13185 (KDD30_13185) - 2855255..2855812 (+) 558 WP_211646265.1 YggT family protein -
  KDD30_RS13190 (KDD30_13190) yggU 2855817..2856104 (+) 288 WP_211646266.1 DUF167 family protein YggU -
  KDD30_RS13195 (KDD30_13195) - 2856230..2856658 (+) 429 WP_211646267.1 DUF4426 domain-containing protein -
  KDD30_RS13200 (KDD30_13200) - 2856785..2857378 (+) 594 WP_211646268.1 XTP/dITP diphosphatase -
  KDD30_RS13205 (KDD30_13205) hemW 2857378..2858544 (+) 1167 WP_211646269.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37516.91 Da        Isoelectric Point: 6.1837

>NTDB_id=559323 KDD30_RS13170 WP_211646262.1 2852629..2853654(-) (pilT) [Photobacterium sp. GJ3]
MDITELLDFSVKHNASDLHLSAGVSPMIRVDGDVRALSIPALTHEAVHRLITDIMDDGQRREYEEHLEVDFSVALPDVGR
FRVNAFRQSRGCAAVFRSIPVNIPTLESLSVPDVFYQIAQSQRGLVLVTGATGSGKSTTIAAMVDHINSTTNRHILTIED
PVEFVHESKRCLINQREVHRDTHSFQNALRSALREDPDVIMVGELRDQETIRLALTAAETGHLVLATLHTSSAAKTIDRI
IDVFPGNDKAMVRSMLSESLRAVIAQSLLKCPGGGRVAAHEIMLATPAIRNLIREDKVAQMYSMIQTGAGMGMQTMEQAV
NRLMAQGLVDPDEVARVLERS

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=559323 KDD30_RS13170 WP_211646262.1 2852629..2853654(-) (pilT) [Photobacterium sp. GJ3]
ATGGATATCACCGAGCTTCTGGACTTTAGTGTAAAGCATAATGCCTCTGATCTGCACCTCTCGGCTGGCGTCTCTCCGAT
GATCCGTGTGGACGGTGATGTCCGGGCGCTGAGTATTCCGGCGCTGACCCATGAAGCTGTGCACCGTTTAATTACGGATA
TTATGGATGACGGACAGCGTCGGGAGTATGAAGAACATCTGGAAGTCGACTTTTCTGTTGCGCTGCCAGACGTGGGACGA
TTCCGGGTGAATGCATTTCGTCAGTCTCGTGGCTGCGCCGCCGTTTTTCGATCGATTCCGGTGAATATCCCCACGCTGGA
ATCCCTCAGCGTGCCCGACGTATTTTATCAGATAGCGCAGAGTCAGCGTGGGCTTGTGTTAGTGACGGGGGCGACAGGTT
CCGGGAAATCAACCACCATTGCTGCCATGGTGGATCACATCAACAGTACCACCAACCGCCATATTCTGACGATCGAAGAT
CCGGTGGAATTTGTGCACGAAAGCAAACGCTGCCTGATCAATCAGCGGGAAGTTCACCGCGATACACACAGTTTCCAGAA
TGCGCTGCGATCGGCACTGCGTGAAGATCCCGATGTGATTATGGTGGGCGAGTTGCGGGATCAGGAAACGATCCGGCTGG
CGCTCACAGCGGCAGAAACCGGGCATCTGGTTTTGGCAACTTTGCATACCAGTTCGGCGGCAAAAACCATTGACCGGATC
ATTGATGTTTTCCCCGGGAACGACAAAGCCATGGTGCGTTCCATGCTGTCTGAATCGCTGCGTGCCGTGATTGCGCAGTC
TTTGCTGAAGTGTCCGGGCGGTGGCCGGGTTGCTGCTCATGAAATTATGCTGGCGACACCCGCCATCCGGAATTTGATCC
GGGAAGACAAAGTGGCTCAGATGTATTCCATGATCCAGACTGGTGCCGGGATGGGCATGCAAACCATGGAGCAAGCGGTC
AACCGGCTGATGGCTCAGGGGCTGGTGGATCCGGATGAAGTGGCGCGTGTGCTGGAACGGAGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.696

99.413

0.762
  pilT Vibrio cholerae strain A1552

76.696

99.413

0.762
  pilT Pseudomonas stutzeri DSM 10701

69.277

97.361

0.674
  pilT Acinetobacter baumannii D1279779

69.486

97.067

0.674
  pilT Acinetobacter baumannii strain A118

69.486

97.067

0.674
  pilT Acinetobacter nosocomialis M2

69.184

97.067

0.672
  pilT Pseudomonas aeruginosa PAK

69.301

96.481

0.669
  pilT Acinetobacter baylyi ADP1

68.788

96.774

0.666
  pilT Legionella pneumophila strain Lp02

66.565

96.481

0.642
  pilT Legionella pneumophila strain ERS1305867

66.565

96.481

0.642
  pilT Neisseria meningitidis 8013

64.095

98.827

0.633
  pilT Neisseria gonorrhoeae MS11

62.281

100

0.625
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.713

100

0.507
  pilU Vibrio cholerae strain A1552

44

95.308

0.419
  pilU Pseudomonas stutzeri DSM 10701

42.378

96.188

0.408
  pilU Acinetobacter baylyi ADP1

40.683

94.428

0.384