Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KDD30_RS13165 Genome accession   NZ_CP073578
Coordinates   2851496..2852599 (-) Length   367 a.a.
NCBI ID   WP_211646261.1    Uniprot ID   -
Organism   Photobacterium sp. GJ3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2846496..2857599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KDD30_RS13140 (KDD30_13140) rsmE 2847002..2847733 (+) 732 WP_211646256.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KDD30_RS13145 (KDD30_13145) gshB 2847749..2848699 (+) 951 WP_211646257.1 glutathione synthase -
  KDD30_RS13150 (KDD30_13150) - 2848884..2849447 (+) 564 WP_211646258.1 YqgE/AlgH family protein -
  KDD30_RS13155 (KDD30_13155) ruvX 2849444..2849872 (+) 429 WP_211646259.1 Holliday junction resolvase RuvX -
  KDD30_RS13160 (KDD30_13160) - 2849994..2851304 (-) 1311 WP_211646260.1 YjiH family protein -
  KDD30_RS13165 (KDD30_13165) pilU 2851496..2852599 (-) 1104 WP_211646261.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KDD30_RS13170 (KDD30_13170) pilT 2852629..2853654 (-) 1026 WP_211646262.1 type IV pilus twitching motility protein PilT Machinery gene
  KDD30_RS13175 (KDD30_13175) - 2853677..2854378 (+) 702 WP_211646263.1 YggS family pyridoxal phosphate-dependent enzyme -
  KDD30_RS13180 (KDD30_13180) proC 2854427..2855245 (+) 819 WP_211646264.1 pyrroline-5-carboxylate reductase -
  KDD30_RS13185 (KDD30_13185) - 2855255..2855812 (+) 558 WP_211646265.1 YggT family protein -
  KDD30_RS13190 (KDD30_13190) yggU 2855817..2856104 (+) 288 WP_211646266.1 DUF167 family protein YggU -
  KDD30_RS13195 (KDD30_13195) - 2856230..2856658 (+) 429 WP_211646267.1 DUF4426 domain-containing protein -
  KDD30_RS13200 (KDD30_13200) - 2856785..2857378 (+) 594 WP_211646268.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 40638.81 Da        Isoelectric Point: 6.8645

>NTDB_id=559322 KDD30_RS13165 WP_211646261.1 2851496..2852599(-) (pilU) [Photobacterium sp. GJ3]
MTLDKLLAEMVARQASDLYLTVAASGQLKVNGELYACGEVLTPAMMTQLCRQAMTDAQWQDFVTHKEANFALVKDARRFR
ISAFWQREQPGMVIRRIEHQIPDVDALTLPEPLKDLAIAKRGLLLVVGATGSGKSTSMAALTGYRNHHSSGHILTVEDPI
EFLHQHDRCIVTQREVGLDTASYAIALKNSLRQAPDMILIGEIRNQETMEYALNFAETGHLCMATLHANNANQALERILH
LVPKERREQLLFDLSLNLKGILAQQLIPDANGAGRHAAFELLLNTPRVSDLIRRGELHALKAVMSKGREHGMMTFDQSLF
ALVQTGKITELDALAHADSANDLRLMMKMDRPFSGSGGLDGVTIRSD

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=559322 KDD30_RS13165 WP_211646261.1 2851496..2852599(-) (pilU) [Photobacterium sp. GJ3]
ATGACGTTAGACAAGCTGCTGGCGGAGATGGTCGCCCGGCAGGCTTCTGATCTTTATCTGACCGTGGCGGCGTCTGGTCA
GCTCAAAGTCAATGGCGAGCTGTATGCCTGCGGGGAAGTTCTGACACCGGCAATGATGACGCAACTGTGTCGGCAGGCGA
TGACTGACGCGCAGTGGCAGGACTTTGTCACACACAAAGAGGCCAACTTTGCGCTGGTGAAGGACGCGCGCCGGTTTCGG
ATCAGTGCGTTCTGGCAGCGGGAACAACCCGGCATGGTGATCCGGCGGATTGAACATCAGATCCCGGATGTGGATGCCCT
GACATTGCCGGAACCCTTGAAAGATCTGGCCATTGCGAAGCGCGGTTTGCTTTTGGTGGTGGGAGCGACCGGATCCGGAA
AATCGACCTCCATGGCAGCCCTGACGGGCTATCGCAATCATCATTCGAGCGGGCATATTCTGACGGTCGAAGATCCGATT
GAGTTTCTCCATCAGCACGACCGGTGCATTGTCACTCAGCGTGAAGTCGGGCTGGACACGGCAAGTTATGCTATCGCGCT
GAAAAACTCCCTGCGTCAGGCGCCGGATATGATTCTGATTGGGGAGATCCGCAATCAGGAAACCATGGAATACGCGCTGA
ATTTTGCAGAAACCGGCCACCTGTGTATGGCGACGCTGCATGCCAACAATGCCAATCAGGCACTGGAGCGGATTCTGCAT
CTAGTCCCGAAAGAACGCCGGGAACAGCTGCTGTTTGATTTATCGCTGAACCTGAAAGGGATTCTGGCGCAGCAATTAAT
ACCTGATGCAAACGGTGCAGGGCGACATGCGGCGTTCGAACTCCTGCTGAACACCCCCAGAGTCTCTGACTTGATTCGCC
GTGGTGAATTGCACGCTCTGAAAGCAGTCATGAGCAAAGGCCGTGAACACGGTATGATGACGTTTGATCAGTCTTTATTC
GCGCTGGTTCAGACCGGAAAAATCACGGAACTCGACGCTCTGGCACATGCCGACTCGGCCAACGACCTCCGGCTAATGAT
GAAAATGGACAGGCCGTTTTCCGGCAGTGGTGGATTGGATGGGGTCACGATCCGCTCAGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(60-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

67.945

99.455

0.676
  pilU Pseudomonas stutzeri DSM 10701

55.84

95.64

0.534
  pilU Acinetobacter baylyi ADP1

54.545

95.913

0.523
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.896

89.101

0.373
  pilT Acinetobacter baylyi ADP1

39.244

93.733

0.368