Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KCX70_RS20400 Genome accession   NZ_CP073105
Coordinates   4452555..4453589 (-) Length   344 a.a.
NCBI ID   WP_021206033.1    Uniprot ID   A0A653VPF0
Organism   Stutzerimonas stutzeri strain F2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4447555..4458589
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCX70_RS20380 (KCX70_20380) - 4448750..4449148 (-) 399 WP_021206029.1 TM2 domain-containing protein -
  KCX70_RS20385 (KCX70_20385) - 4449294..4450505 (+) 1212 WP_212618637.1 putative DNA modification/repair radical SAM protein -
  KCX70_RS20390 (KCX70_20390) - 4450505..4451335 (+) 831 WP_212618638.1 TIGR03915 family putative DNA repair protein -
  KCX70_RS20395 (KCX70_20395) pilU 4451336..4452481 (-) 1146 WP_021206032.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KCX70_RS20400 (KCX70_20400) pilT 4452555..4453589 (-) 1035 WP_021206033.1 type IV pilus twitching motility protein PilT Machinery gene
  KCX70_RS20405 (KCX70_20405) - 4453656..4454348 (+) 693 WP_212618639.1 YggS family pyridoxal phosphate-dependent enzyme -
  KCX70_RS20410 (KCX70_20410) proC 4454365..4455192 (+) 828 WP_212618640.1 pyrroline-5-carboxylate reductase -
  KCX70_RS20415 (KCX70_20415) - 4455526..4456116 (+) 591 WP_021206036.1 YggT family protein -
  KCX70_RS20420 (KCX70_20420) - 4456225..4458186 (+) 1962 WP_212618641.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37930.67 Da        Isoelectric Point: 6.8360

>NTDB_id=557962 KCX70_RS20400 WP_021206033.1 4452555..4453589(-) (pilT) [Stutzerimonas stutzeri strain F2]
MDITELLAFSAKQGASDLHLSSGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGSLGMQTLDACL
KGLLAKGLISRDSAKEKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=557962 KCX70_RS20400 WP_021206033.1 4452555..4453589(-) (pilT) [Stutzerimonas stutzeri strain F2]
ATGGATATCACAGAGCTGCTGGCCTTCAGTGCCAAGCAGGGTGCGTCGGATTTGCACCTGTCTTCCGGCTTGCCGCCGAT
GATCCGCGTCGATGGCGACGTGCGTCGGATCAACCTCCCCGCGATGGACCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAATGACAAGCAGCGCAAGGATTTCGAGGAATTTCTCGAGACCGACTTCTCCTTCGAGGTTCCTGGCGTGGCCCGC
TTCCGGGTCAACGCGTTCAACCAGAACCGTGGCGCCGGCGCGGTCTTCCGGACCATTCCGTCCAAGGTGCTGACCATGGA
GGACCTCGGCATGGGCGAAGTCTTCCGCAAGATCACCGACGTACCGCGCGGCCTGGTGCTGGTCACCGGCCCGACCGGTT
CGGGCAAGTCCACCACCCTGGCGGCGATGCTCGACTACCTGAACAACACCAAGTACCACCATATCCTCACCATTGAGGAC
CCGATCGAATTCGTCCACGAATCGAAGAAGTGCCTGGTCAACCAGCGCGAAGTGCACCGTGACACCCTCGGCTTCTCCGA
GGCGCTGCGTTCGGCGCTGCGGGAAGACCCGGACATCATCCTGGTGGGCGAAATGCGCGACCTGGAAACCATTCGCCTCG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTATTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGCGTG
GTCGACGTGTTTCCGGCGGAAGAAAAGTCCATGGTCCGTTCGATGCTGTCCGAGTCGCTGCAGGCGGTGATTTCCCAGAC
GCTGCTGAAGAAAGTCGGTGGCGGCCGGGTCGCGGCCCACGAAATCATGATCGGCACCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCCTCGATTCAGACCGGCGGATCGCTGGGCATGCAGACGCTCGACGCCTGTCTC
AAGGGTCTACTGGCCAAGGGTCTGATTTCCCGCGACAGCGCCAAGGAAAAGGCCAAGCAGCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A653VPF0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

99.709

100

0.997
  pilT Pseudomonas aeruginosa PAK

94.186

100

0.942
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

83.43

100

0.834
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Vibrio cholerae strain A1552

41.003

98.547

0.404
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392