Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KCX70_RS20395 Genome accession   NZ_CP073105
Coordinates   4451336..4452481 (-) Length   381 a.a.
NCBI ID   WP_021206032.1    Uniprot ID   A0A2N8SST2
Organism   Stutzerimonas stutzeri strain F2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4446336..4457481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCX70_RS20370 (KCX70_20370) - 4446427..4447443 (+) 1017 WP_212618635.1 aspartate carbamoyltransferase catalytic subunit -
  KCX70_RS20375 (KCX70_20375) - 4447443..4448714 (+) 1272 WP_212618636.1 dihydroorotase -
  KCX70_RS20380 (KCX70_20380) - 4448750..4449148 (-) 399 WP_021206029.1 TM2 domain-containing protein -
  KCX70_RS20385 (KCX70_20385) - 4449294..4450505 (+) 1212 WP_212618637.1 putative DNA modification/repair radical SAM protein -
  KCX70_RS20390 (KCX70_20390) - 4450505..4451335 (+) 831 WP_212618638.1 TIGR03915 family putative DNA repair protein -
  KCX70_RS20395 (KCX70_20395) pilU 4451336..4452481 (-) 1146 WP_021206032.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KCX70_RS20400 (KCX70_20400) pilT 4452555..4453589 (-) 1035 WP_021206033.1 type IV pilus twitching motility protein PilT Machinery gene
  KCX70_RS20405 (KCX70_20405) - 4453656..4454348 (+) 693 WP_212618639.1 YggS family pyridoxal phosphate-dependent enzyme -
  KCX70_RS20410 (KCX70_20410) proC 4454365..4455192 (+) 828 WP_212618640.1 pyrroline-5-carboxylate reductase -
  KCX70_RS20415 (KCX70_20415) - 4455526..4456116 (+) 591 WP_021206036.1 YggT family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42450.68 Da        Isoelectric Point: 6.5052

>NTDB_id=557961 KCX70_RS20395 WP_021206032.1 4451336..4452481(-) (pilU) [Stutzerimonas stutzeri strain F2]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVNKTPMSPEMTRETVHSVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTLEDLKLPDVLKKLAMTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEFIHQHQSCIVTQREVGIDTDSFDVALKNTLRQAPDVILIGEVRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTDLGMQTFDQA
LYNLYVQGEITYEDALLHADSSNDLRLMIKLGSETDGEHLTSVSQGLTLEVSDDDPGRSFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=557961 KCX70_RS20395 WP_021206032.1 4451336..4452481(-) (pilU) [Stutzerimonas stutzeri strain F2]
ATGGAATTCGAGAAACTGTTGCGTCTGATGGTCGAGAAAGGCGGCTCCGATCTGTTCATTACCGCTGGTGTGCCGCCTTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAACAAGACGCCGATGTCGCCGGAGATGACCCGCGAAACCGTGCACTCGG
TGATGAACGAGCAGCAACGCCGGGACTTCGCCGAGAACCACGAATGCAATTTCGCCATCAGCGCGCGCGGTATCGGCCGC
TTCCGTGTCAGTGCCTTCTATCAGCGCAACCTTGCCGGCATGGTGCTGCGTCGTATCGAGACCAATATCCCGACGCTCGA
AGATCTGAAGCTGCCCGACGTGCTGAAGAAACTGGCCATGACCAAGCGTGGCCTGGTGCTGTTCGTCGGTGCGACCGGCA
CCGGCAAGTCCACCTCCCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCAGCGGTCACATCATTTCCATCGAAGAC
CCCATCGAGTTCATTCACCAGCATCAAAGCTGCATCGTCACCCAGCGTGAGGTCGGCATCGACACCGACTCCTTCGACGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCCGACGTCATCCTCATCGGCGAGGTGCGGACCCGCGAGACCATGGACTACG
CCGTGGCCTTCGCCGAAACCGGCCACCTCTGCCTCGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGCTGATCGTCAGCAGCAGGTCTGGATGGACCTGTCGCTTAACTTGAAAGCCATCGTCGCCCAGCA
GCTGATCCCGACCCCGGATGGCAAGGGGCGCCGCGCGGTCATCGAAGTCCTGATCAACACGCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAAGTGCACGAGCTCAAATCGCTGATGAAGCGCTCCACCGACCTCGGCATGCAGACCTTCGATCAGGCG
CTGTACAACCTCTACGTGCAGGGTGAAATCACCTACGAAGACGCGCTGCTGCATGCAGACTCGTCCAACGACCTGCGCCT
GATGATCAAGCTGGGCTCGGAAACCGATGGCGAGCATCTGACCTCCGTGTCCCAGGGCCTCACTCTGGAAGTCAGCGACG
ACGATCCGGGTCGCAGTTTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N8SST2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

96.588

100

0.966
  pilU Acinetobacter baylyi ADP1

66.106

93.701

0.619
  pilU Vibrio cholerae strain A1552

58.571

91.864

0.538
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.248

88.976

0.394
  pilT Neisseria meningitidis 8013

41.159

90.551

0.373
  pilT Neisseria gonorrhoeae MS11

41.159

90.551

0.373
  pilT Legionella pneumophila strain ERS1305867

41.791

87.927

0.367
  pilT Pseudomonas aeruginosa PAK

41.791

87.927

0.367
  pilT Legionella pneumophila strain Lp02

41.791

87.927

0.367
  pilT Pseudomonas stutzeri DSM 10701

41.493

87.927

0.365
  pilT Vibrio cholerae strain A1552

41.124

88.714

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.124

88.714

0.365
  pilT Acinetobacter baumannii strain A118

41.194

87.927

0.362
  pilT Acinetobacter baumannii D1279779

41.194

87.927

0.362
  pilT Acinetobacter nosocomialis M2

41.194

87.927

0.362