Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J9318_RS06950 Genome accession   NZ_CP072844
Coordinates   1517436..1518494 (+) Length   352 a.a.
NCBI ID   WP_210562309.1    Uniprot ID   -
Organism   Psychrosphaera aestuarii strain PSC101     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1512436..1523494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J9318_RS06905 rlmH 1512501..1512971 (-) 471 WP_210562301.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  J9318_RS06910 rsfS 1512978..1513307 (-) 330 WP_210562302.1 ribosome silencing factor -
  J9318_RS06915 - 1513388..1513714 (-) 327 WP_210562303.1 DUF962 domain-containing protein -
  J9318_RS06920 - 1513726..1514367 (-) 642 WP_210562304.1 GNAT family N-acetyltransferase -
  J9318_RS06925 - 1514500..1514730 (+) 231 WP_210562305.1 DUF2789 domain-containing protein -
  J9318_RS06930 - 1514837..1515268 (-) 432 WP_244731973.1 DUF4426 domain-containing protein -
  J9318_RS06935 - 1515281..1515823 (-) 543 WP_210562306.1 YggT family protein -
  J9318_RS06940 proC 1515825..1516670 (-) 846 WP_210562307.1 pyrroline-5-carboxylate reductase -
  J9318_RS06945 - 1516695..1517402 (-) 708 WP_210562308.1 YggS family pyridoxal phosphate-dependent enzyme -
  J9318_RS06950 pilT 1517436..1518494 (+) 1059 WP_210562309.1 type IV pilus twitching motility protein PilT Machinery gene
  J9318_RS06955 pilU 1518504..1519628 (+) 1125 WP_210562310.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J9318_RS06960 rne 1519733..1522726 (-) 2994 WP_210562311.1 ribonuclease E -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38903.26 Da        Isoelectric Point: 5.7517

>NTDB_id=556152 J9318_RS06950 WP_210562309.1 1517436..1518494(+) (pilT) [Psychrosphaera aestuarii strain PSC101]
MDITELLAFSVQHNASDLHLSAGTPPMIRVDGDVRRVNIPALDAREVQSLIYDIMNDKQRKEYEENLETDFSFAVPDLAR
FRVNAFNMDRGAAAVFRTIPSEVLSLEDIGAPDIFKDIAENPRGLVLVTGPTGSGKSTTLAAMIDYINTQRHDHILTIED
PIEFVHQNKQSLISQREVHRDTLSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
VDVFPGDEKDMVRSMLSESLRAVISQTLCKKTGGGRVAAHEIMIGIPAIRNLIREDKVAQMYSSIQTGAAHGMQTMDQCL
LNLMNRGLVSREHAQAKAHDKNLFSQGFGGGF

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=556152 J9318_RS06950 WP_210562309.1 1517436..1518494(+) (pilT) [Psychrosphaera aestuarii strain PSC101]
ATGGATATTACTGAATTACTTGCCTTTAGTGTGCAACATAATGCTTCAGATTTACACCTTTCAGCAGGTACTCCTCCAAT
GATACGAGTTGACGGTGACGTCAGACGAGTAAACATACCGGCATTAGATGCTAGAGAAGTACAATCGCTTATTTACGATA
TTATGAATGATAAACAGCGTAAAGAGTACGAAGAAAACTTAGAAACGGATTTTTCTTTTGCAGTTCCTGACCTCGCTCGT
TTTCGTGTTAATGCATTTAACATGGATAGAGGCGCAGCTGCCGTGTTTAGGACTATCCCATCAGAAGTATTAAGTCTAGA
AGATATTGGCGCCCCTGATATTTTTAAAGATATAGCGGAAAACCCTAGAGGACTTGTTTTAGTTACTGGTCCAACGGGAT
CGGGTAAGTCAACGACGTTAGCGGCAATGATTGATTACATCAATACTCAGCGCCACGACCACATATTGACCATCGAGGAT
CCAATCGAATTTGTGCATCAAAATAAGCAAAGTTTGATTAGTCAGCGAGAAGTACATAGAGATACACTTAGCTTTAGTAA
CGCCTTACGTTCTGCTCTTCGTGAGGATCCAGACGTTATTCTTGTTGGTGAATTACGAGATTTAGAAACTATCAGACTGG
CAATGACAGCCGCGGAAACCGGCCACTTGGTTTTCGGCACACTACATACAACATCGGCACCAAAAACTATCGACCGTATT
GTCGACGTATTCCCAGGTGATGAAAAAGACATGGTTCGTTCTATGTTATCTGAGTCGTTAAGAGCAGTAATATCGCAAAC
ACTTTGTAAAAAAACCGGTGGCGGTCGAGTTGCGGCTCATGAAATTATGATTGGTATTCCAGCGATTCGTAACCTTATCC
GAGAAGATAAGGTCGCGCAGATGTATTCATCGATTCAAACGGGAGCAGCGCACGGCATGCAAACTATGGATCAATGTTTA
TTGAACCTGATGAATAGAGGTTTGGTGAGTAGAGAGCACGCTCAAGCAAAAGCTCATGATAAAAATTTATTCAGCCAAGG
GTTTGGCGGCGGATTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.326

97.727

0.756
  pilT Acinetobacter nosocomialis M2

76.163

97.727

0.744
  pilT Acinetobacter baumannii D1279779

76.163

97.727

0.744
  pilT Acinetobacter baumannii strain A118

76.163

97.727

0.744
  pilT Pseudomonas stutzeri DSM 10701

75.872

97.727

0.741
  pilT Pseudomonas aeruginosa PAK

76.331

96.023

0.733
  pilT Legionella pneumophila strain Lp02

72.965

97.727

0.713
  pilT Legionella pneumophila strain ERS1305867

72.965

97.727

0.713
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.513

95.739

0.685
  pilT Vibrio cholerae strain A1552

71.513

95.739

0.685
  pilT Neisseria meningitidis 8013

68.497

98.295

0.673
  pilT Neisseria gonorrhoeae MS11

68.208

98.295

0.67
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.482

100

0.545
  pilU Pseudomonas stutzeri DSM 10701

41.108

97.443

0.401
  pilU Vibrio cholerae strain A1552

39.942

97.443

0.389
  pilU Acinetobacter baylyi ADP1

39.535

97.727

0.386