Detailed information NTG0556153

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: J9318_RS06955
• Genome accession: NZ_CP072844
• Coordinates: 1518504..1519628 (+)
• Length: 374 a.a.
• NCBI ID: WP_210562310.1
• Uniprot ID: -
• Organism: Psychrosphaera aestuarii strain PSC101
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1513504..1524628

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J9318_RS06920	-	1513726..1514367 (-)	642	WP_210562304.1	GNAT family N-acetyltransferase	-
J9318_RS06925	-	1514500..1514730 (+)	231	WP_210562305.1	DUF2789 domain-containing protein	-
J9318_RS06930	-	1514837..1515268 (-)	432	WP_244731973.1	DUF4426 domain-containing protein	-
J9318_RS06935	-	1515281..1515823 (-)	543	WP_210562306.1	YggT family protein	-
J9318_RS06940	proC	1515825..1516670 (-)	846	WP_210562307.1	pyrroline-5-carboxylate reductase	-
J9318_RS06945	-	1516695..1517402 (-)	708	WP_210562308.1	YggS family pyridoxal phosphate-dependent enzyme	-
J9318_RS06950	pilT	1517436..1518494 (+)	1059	WP_210562309.1	type IV pilus twitching motility protein PilT	Machinery gene
J9318_RS06955	pilU	1518504..1519628 (+)	1125	WP_210562310.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
J9318_RS06960	rne	1519733..1522726 (-)	2994	WP_210562311.1	ribonuclease E	-
J9318_RS06965	rluC	1523263..1524234 (+)	972	WP_210562401.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Sequence

Protein

• Length: 374 a.a.
• Molecular weight: 41445.44 Da
• Isoelectric Point: 5.6362

>NTDB_id=556153 J9318_RS06955 WP_210562310.1 1518504..1519628(+) (pilU) [Psychrosphaera aestuarii strain PSC101]
MESMEQIFNKMVAEKASDLFITAGLPISAKINGELTPLDHDALTEFEAERLVRSCMSDKQQNEFDTEKECNFAVANDAGR
FRVSAFWQRDNVGMVVRRIVTNIPDVDELGLPAALKDVIMAKRGLVLFVGGTGTGKSTSLAALLGYRNRNSKGHILTIED
PIEFVHEHKRCMITQREVGIDTPSFESALKSSLRQAPDVILIGEIRSQEIMEYALSFAETGHLCVATLHANNANQAIDRI
MHLVPKEKHDKLKYDLALNLKAIVAQQLIQTSDGMGRVAAIEVLLNSPMVGELIKKGDISGIKEVMAKSKEFGMQTFDQA
LFNLYQESRISYADALHHADSPNDLRLMIKLRNNEQTGSGFLQGVTVDGIQKDD

Nucleotide

• Length: 1125 bp

>NTDB_id=556153 J9318_RS06955 WP_210562310.1 1518504..1519628(+) (pilU) [Psychrosphaera aestuarii strain PSC101]
ATGGAATCGATGGAGCAAATATTTAATAAAATGGTGGCGGAAAAAGCGTCAGACCTTTTTATTACAGCTGGACTACCCAT
AAGTGCAAAGATCAATGGTGAGTTAACACCATTAGATCATGATGCTTTAACTGAGTTTGAAGCAGAACGACTCGTTCGCA
GCTGCATGTCAGATAAACAACAAAATGAATTTGATACTGAAAAAGAATGTAACTTTGCCGTAGCAAACGATGCAGGACGT
TTTCGTGTTTCAGCCTTTTGGCAAAGAGATAATGTCGGCATGGTGGTTCGCCGAATTGTGACTAACATTCCTGATGTAGA
TGAGCTTGGTTTACCAGCGGCGTTAAAAGACGTCATTATGGCAAAGCGTGGTCTTGTATTGTTCGTTGGTGGTACTGGCA
CAGGTAAGTCAACTAGCTTGGCAGCTTTATTAGGTTACCGAAATCGTAATTCCAAAGGACATATCTTAACAATAGAAGAT
CCAATCGAATTTGTTCATGAACATAAACGATGCATGATAACACAACGTGAAGTTGGCATAGATACACCATCTTTTGAATC
GGCGTTAAAGAGCTCATTACGACAAGCGCCAGATGTGATTCTGATCGGTGAGATTCGATCGCAAGAAATAATGGAATACG
CTCTATCATTTGCGGAAACTGGGCACTTGTGTGTTGCTACGTTGCACGCGAACAACGCGAACCAAGCAATAGATCGCATT
ATGCATTTGGTACCAAAAGAAAAGCACGACAAACTTAAATATGACTTAGCGTTAAATTTAAAGGCGATCGTTGCCCAGCA
ACTTATTCAAACGAGTGATGGAATGGGACGAGTGGCTGCAATCGAAGTTTTATTAAACTCGCCTATGGTAGGTGAACTAA
TTAAAAAAGGTGATATTTCTGGTATCAAAGAAGTGATGGCGAAATCAAAAGAGTTTGGTATGCAAACTTTTGATCAGGCG
CTGTTTAACCTTTACCAAGAGTCTCGAATTAGTTATGCCGATGCTTTGCACCATGCAGATTCGCCAAATGACCTACGCCT
AATGATAAAATTAAGGAATAATGAGCAAACCGGTTCAGGTTTCCTTCAAGGTGTTACCGTCGATGGCATTCAAAAAGACG
ATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (9-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	57.979	100	0.583
pilU	Vibrio cholerae strain A1552	58.082	97.594	0.567
pilU	Pseudomonas stutzeri DSM 10701	59.599	93.316	0.556
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.909	94.385	0.414
pilT	Acinetobacter baumannii strain A118	40.663	88.77	0.361
pilT	Acinetobacter nosocomialis M2	40.663	88.77	0.361
pilT	Acinetobacter baylyi ADP1	40.663	88.77	0.361
pilT	Acinetobacter baumannii D1279779	40.663	88.77	0.361