Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J9253_RS18085 Genome accession   NZ_CP072801
Coordinates   3742456..3743490 (-) Length   344 a.a.
NCBI ID   WP_210222255.1    Uniprot ID   -
Organism   Thiothrix litoralis strain AS     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3737456..3748490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J9253_RS18060 (J9253_18060) - 3737577..3738071 (-) 495 WP_210222251.1 DUF1566 domain-containing protein -
  J9253_RS21385 - 3738388..3738888 (-) 501 WP_407701776.1 RNA-guided endonuclease InsQ/TnpB family protein -
  J9253_RS21390 - 3738899..3739336 (-) 438 WP_407701777.1 hypothetical protein -
  J9253_RS18070 (J9253_18070) bioD 3739486..3740160 (+) 675 WP_210222252.1 dethiobiotin synthase -
  J9253_RS18075 (J9253_18075) - 3740143..3741288 (-) 1146 WP_210222253.1 PilT/PilU family type 4a pilus ATPase -
  J9253_RS18080 (J9253_18080) pilU 3741288..3742442 (-) 1155 WP_210222254.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J9253_RS18085 (J9253_18085) pilT 3742456..3743490 (-) 1035 WP_210222255.1 type IV pilus twitching motility protein PilT Machinery gene
  J9253_RS18090 (J9253_18090) - 3743552..3744232 (+) 681 WP_210222256.1 YggS family pyridoxal phosphate-dependent enzyme -
  J9253_RS18095 (J9253_18095) - 3744244..3744789 (-) 546 WP_210222257.1 DUF4124 domain-containing protein -
  J9253_RS18100 (J9253_18100) yegQ 3744894..3746249 (-) 1356 WP_210222258.1 tRNA 5-hydroxyuridine modification protein YegQ -
  J9253_RS18105 (J9253_18105) - 3746255..3746836 (-) 582 WP_210222259.1 thioredoxin family protein -
  J9253_RS18110 (J9253_18110) - 3746970..3747929 (+) 960 WP_210222260.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38185.72 Da        Isoelectric Point: 8.1677

>NTDB_id=555931 J9253_RS18085 WP_210222255.1 3742456..3743490(-) (pilT) [Thiothrix litoralis strain AS]
MDITQLLAFSVKNNASDLHLSGGMPPMIRVDGEMRKINLPVLDHKQVHALVYDIMNDHQRKNYEEHWECDFSFEIPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTLDQLGAPGIFRKISENPRGLVLVTGPTGSGKSTTLAAMVDYKNDTDYGHILTIED
PIEFVHESKKSLVNQREVHRDTKGFEAALRSALREDPDTILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAPKTIDRI
VDVFPAAEKEMVRSMLSESLQAVISQTLLKKKGGGRVAAHEILIGTRAVRNLIRENKIAQMRSAMQTGQSDGMQTLDQSL
KNLVLKGLVDRETARRKADNPDSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=555931 J9253_RS18085 WP_210222255.1 3742456..3743490(-) (pilT) [Thiothrix litoralis strain AS]
ATGGATATTACCCAACTGCTGGCGTTCAGCGTCAAAAACAATGCTTCCGACTTACACTTGTCGGGGGGGATGCCACCGAT
GATCCGTGTCGATGGCGAAATGCGCAAGATCAACCTACCCGTGCTTGACCACAAACAGGTACACGCATTGGTTTACGACA
TCATGAACGATCACCAGCGCAAAAACTACGAAGAACACTGGGAATGCGATTTCTCGTTTGAAATCCCCGGCGTGGCACGT
TTCCGGGTCAATGCCTTCAACCAGAATCGTGGTGCAGGTGCCGTCTTCCGTACCATCCCCAGCAAGGTACTGACGCTCGA
CCAACTCGGTGCTCCCGGTATTTTCCGCAAAATTTCCGAAAATCCACGCGGTTTGGTGTTAGTGACGGGACCCACAGGTT
CAGGCAAATCCACCACGCTGGCAGCAATGGTCGATTACAAAAACGATACCGATTATGGGCACATCCTCACCATTGAAGAC
CCAATCGAATTTGTCCACGAAAGCAAAAAAAGTCTGGTCAACCAACGGGAAGTCCACCGCGACACCAAAGGCTTTGAAGC
CGCCTTGCGTTCCGCCTTGCGTGAAGACCCGGACACCATTCTGGTTGGGGAAATGCGTGACCTCGAAACCATCCGCCTTG
CCCTGACCGCTGCCGAAACCGGGCATTTGGTGTTCGGTACCTTGCACACCACTTCCGCCCCCAAAACCATCGACCGTATT
GTCGACGTATTCCCGGCAGCGGAAAAGGAAATGGTGCGTTCGATGCTTTCCGAATCCCTGCAAGCGGTCATCTCCCAAAC
CCTGCTGAAGAAAAAAGGCGGCGGACGGGTAGCAGCACACGAAATCCTCATCGGCACACGTGCAGTACGTAACCTGATCC
GCGAAAACAAAATTGCACAAATGCGCTCCGCAATGCAAACCGGGCAAAGTGATGGGATGCAAACCCTTGACCAGTCACTC
AAAAATCTGGTGCTGAAAGGGCTAGTCGATCGGGAAACCGCCCGCCGCAAAGCCGACAACCCGGACTCCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.651

100

0.797
  pilT Pseudomonas aeruginosa PAK

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

78.198

100

0.782
  pilT Acinetobacter baumannii strain A118

78.198

100

0.782
  pilT Acinetobacter nosocomialis M2

78.198

100

0.782
  pilT Acinetobacter baylyi ADP1

76.744

100

0.767
  pilT Legionella pneumophila strain Lp02

72.353

98.837

0.715
  pilT Legionella pneumophila strain ERS1305867

72.353

98.837

0.715
  pilT Neisseria meningitidis 8013

71.304

100

0.715
  pilT Neisseria gonorrhoeae MS11

71.014

100

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.552

98.547

0.666
  pilT Vibrio cholerae strain A1552

67.552

98.547

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.462

99.419

0.512
  pilU Vibrio cholerae strain A1552

40.87

100

0.41
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398