Detailed information NTG0555930

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: J9253_RS18080
• Genome accession: NZ_CP072801
• Coordinates: 3741288..3742442 (-)
• Length: 384 a.a.
• NCBI ID: WP_210222254.1
• Uniprot ID: -
• Organism: Thiothrix litoralis strain AS
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3736288..3747442

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J9253_RS18060 (J9253_18060)	-	3737577..3738071 (-)	495	WP_210222251.1	DUF1566 domain-containing protein	-
J9253_RS21385	-	3738388..3738888 (-)	501	WP_407701776.1	RNA-guided endonuclease InsQ/TnpB family protein	-
J9253_RS21390	-	3738899..3739336 (-)	438	WP_407701777.1	hypothetical protein	-
J9253_RS18070 (J9253_18070)	bioD	3739486..3740160 (+)	675	WP_210222252.1	dethiobiotin synthase	-
J9253_RS18075 (J9253_18075)	-	3740143..3741288 (-)	1146	WP_210222253.1	PilT/PilU family type 4a pilus ATPase	-
J9253_RS18080 (J9253_18080)	pilU	3741288..3742442 (-)	1155	WP_210222254.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
J9253_RS18085 (J9253_18085)	pilT	3742456..3743490 (-)	1035	WP_210222255.1	type IV pilus twitching motility protein PilT	Machinery gene
J9253_RS18090 (J9253_18090)	-	3743552..3744232 (+)	681	WP_210222256.1	YggS family pyridoxal phosphate-dependent enzyme	-
J9253_RS18095 (J9253_18095)	-	3744244..3744789 (-)	546	WP_210222257.1	DUF4124 domain-containing protein	-
J9253_RS18100 (J9253_18100)	yegQ	3744894..3746249 (-)	1356	WP_210222258.1	tRNA 5-hydroxyuridine modification protein YegQ	-
J9253_RS18105 (J9253_18105)	-	3746255..3746836 (-)	582	WP_210222259.1	thioredoxin family protein	-

Sequence

Protein

• Length: 384 a.a.
• Molecular weight: 42903.51 Da
• Isoelectric Point: 7.2741

>NTDB_id=555930 J9253_RS18080 WP_210222254.1 3741288..3742442(-) (pilU) [Thiothrix litoralis strain AS]
MDRDAAVSYVHKLLATMLEKNASDLYITADAAPSAKIASEMLPLTTQALNEQNARMLVRAIMNDRQLKQFEEEMECNFSI
SLPGKARFRVNAFTQRGCAGMVLRHIPSHIPGLKDLGLPPVLRDIAMTKRGLVIMVGATGSGKSTTLASMVDFRNTHSKG
HIITIEDPIEFVHQHKGCLITQREIGVDTANYEIAMKNTLRQAPDVILLGEIRDRESMDYAIAYAETGHLCLTTLHANSS
NQAIDRIINFFPEERRNQLLMDLSLNLKAVVSQRLVRKAKGEGRLAAVEVVINTPLMADLILKGDVPGMKVLTSKSREHG
MRTFDQALFDLIEARQITVKEALRHADSQNDLRLRLKLESRFAQDNDFFKGLDDLTIEPMENAN

Nucleotide

• Length: 1155 bp

>NTDB_id=555930 J9253_RS18080 WP_210222254.1 3741288..3742442(-) (pilU) [Thiothrix litoralis strain AS]
ATGGATCGCGATGCCGCCGTCAGCTATGTCCACAAATTGCTGGCCACCATGTTGGAAAAAAACGCTTCCGACTTGTATAT
CACGGCGGATGCCGCGCCTTCCGCCAAAATTGCCAGCGAAATGCTGCCACTGACCACACAGGCATTGAATGAGCAAAATG
CCCGCATGTTGGTACGTGCCATCATGAATGACCGCCAGCTCAAACAGTTTGAGGAAGAGATGGAATGTAACTTTTCCATC
AGCCTGCCCGGCAAGGCACGTTTCCGGGTGAATGCCTTCACCCAGCGTGGTTGTGCAGGCATGGTGCTGCGGCATATTCC
CAGCCATATACCCGGCCTGAAAGACTTGGGGCTACCGCCGGTATTACGCGACATCGCCATGACCAAACGCGGGCTGGTGA
TCATGGTTGGTGCGACCGGCTCGGGTAAATCCACCACGCTGGCGTCGATGGTCGACTTCCGCAATACCCATTCCAAGGGT
CATATCATTACCATTGAAGACCCGATTGAATTTGTCCACCAGCACAAAGGCTGCCTGATTACCCAACGGGAAATCGGGGT
CGATACTGCCAATTACGAAATAGCCATGAAGAACACCCTGCGGCAAGCACCCGACGTCATCCTGCTCGGGGAAATCCGTG
ACCGCGAGTCGATGGATTACGCGATTGCCTATGCTGAAACCGGGCATCTGTGCCTGACGACCTTACACGCCAATAGTTCC
AACCAAGCCATTGACCGCATCATCAACTTTTTCCCGGAAGAACGCCGCAACCAGTTATTGATGGATTTATCCCTCAACCT
GAAAGCCGTGGTATCACAACGGCTGGTGCGCAAAGCCAAGGGCGAAGGGCGTTTAGCTGCCGTGGAAGTGGTGATCAATA
CCCCGCTGATGGCTGATCTAATCCTCAAAGGCGACGTACCGGGGATGAAAGTACTCACCTCCAAATCCCGCGAACACGGG
ATGCGTACTTTCGACCAAGCCCTGTTTGACCTGATCGAAGCCCGCCAGATTACCGTGAAAGAAGCCTTGCGCCATGCTGA
TTCCCAAAATGACCTACGCCTGCGCCTCAAACTGGAAAGCCGCTTTGCCCAAGACAACGACTTTTTCAAGGGTCTGGACG
ACCTCACCATTGAACCCATGGAGAACGCCAACTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (14-278)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	59.312	90.885	0.539
pilU	Acinetobacter baylyi ADP1	56.772	90.365	0.513
pilU	Vibrio cholerae strain A1552	51.149	90.625	0.464
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.659	94.01	0.401
pilT	Acinetobacter baumannii D1279779	43.824	88.542	0.388
pilT	Acinetobacter baumannii strain A118	43.824	88.542	0.388
pilT	Acinetobacter nosocomialis M2	43.529	88.542	0.385
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.834	90.885	0.38
pilT	Vibrio cholerae strain A1552	41.834	90.885	0.38
pilT	Acinetobacter baylyi ADP1	44.892	84.115	0.378
pilT	Pseudomonas aeruginosa PAK	42.09	87.24	0.367
pilT	Legionella pneumophila strain ERS1305867	42.857	85.677	0.367
pilT	Legionella pneumophila strain Lp02	42.857	85.677	0.367
pilT	Pseudomonas stutzeri DSM 10701	43.653	84.115	0.367