NTDB

Overview

Name	pilA2	Type	Machinery gene
Locus tag	J8380_RS06130	Genome accession	NZ_CP072800
Coordinates	1211017..1211457 (-)	Length	146 a.a.
NCBI ID	WP_210230560.1	Uniprot ID	-
Organism	Candidatus Thiothrix anitrata isolate A52
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1206017..1216457

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J8380_RS06115 (J8380_06115)	pilB	1206675..1208384 (-)	1710	WP_210229265.1	type IV-A pilus assembly ATPase PilB	Machinery gene
J8380_RS06120 (J8380_06120)	tfpZ	1208696..1209466 (-)	771	WP_210229266.1	TfpX/TfpZ family type IV pilin accessory protein	-
J8380_RS06125 (J8380_06125)	-	1209484..1210890 (-)	1407	WP_210229268.1	O-antigen ligase	-
J8380_RS06130 (J8380_06130)	pilA2	1211017..1211457 (-)	441	WP_210230560.1	pilin	Machinery gene
J8380_RS06135 (J8380_06135)	-	1211726..1212478 (+)	753	WP_210229270.1	ATP-binding protein	-
J8380_RS06140 (J8380_06140)	acs	1212629..1214578 (+)	1950	WP_210229273.1	acetate--CoA ligase	-
J8380_RS06145 (J8380_06145)	-	1214703..1215005 (-)	303	WP_210229275.1	DUF2288 domain-containing protein	-
J8380_RS06150 (J8380_06150)	-	1215073..1216161 (-)	1089	WP_210229277.1	glycosyltransferase	-

Sequence

Protein

Download Length: 146 a.a. Molecular weight: 14889.05 Da Isoelectric Point: 9.5386

                                >NTDB_id=555904 J8380_RS06130 WP_210230560.1 1211017..1211457(-) (pilA2) [Candidatus Thiothrix anitrata isolate A52]

MKAKAQQGFTLIELMIVVAIIGILAAIALPAYQDYTKRAHVSEGLSLAGSAKLAVTEFYADRGAWPATNTAAGLPAATSI

TGNSVTSVGVGNATGIITVTYNAKVQNGTTIQLTPTVADANGSITWSCTGGTVLAKFRPATCRGTN

Nucleotide

Download Length: 441 bp

                                >NTDB_id=555904 J8380_RS06130 WP_210230560.1 1211017..1211457(-) (pilA2) [Candidatus Thiothrix anitrata isolate A52]

ATGAAAGCAAAAGCCCAACAAGGCTTCACCCTGATCGAATTGATGATCGTTGTCGCCATCATCGGCATTCTGGCAGCGAT

TGCACTGCCTGCTTACCAAGACTACACCAAGCGTGCGCATGTTTCGGAAGGATTGAGCTTAGCAGGCTCTGCCAAACTGG

CTGTTACAGAGTTTTATGCCGACCGTGGCGCATGGCCTGCAACCAATACTGCTGCGGGTCTACCTGCTGCTACGAGCATC

ACTGGTAACTCAGTTACCTCCGTTGGTGTTGGTAACGCAACCGGTATCATAACTGTTACGTATAACGCCAAAGTCCAAAA

CGGTACAACAATCCAACTGACTCCCACCGTTGCTGATGCAAATGGCTCTATCACTTGGAGCTGCACAGGTGGTACTGTTC

TTGCTAAATTCCGTCCTGCCACTTGCCGTGGTACAAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)

Pilin (bacterial filament) (Pilin)

(38-143)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA2	Legionella pneumophila str. Paris	62.857	95.89	0.603
pilA2	Legionella pneumophila strain ERS1305867	62.857	95.89	0.603
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	40.212	100	0.521
pilA	Ralstonia pseudosolanacearum GMI1000	43.275	100	0.507
pilA	Vibrio campbellii strain DS40M4	47.02	100	0.486
pilE	Neisseria gonorrhoeae MS11	45.223	100	0.486
comP	Acinetobacter baylyi ADP1	47.973	100	0.486
pilE	Neisseria gonorrhoeae strain FA1090	44.025	100	0.479
pilA/pilA1	Eikenella corrodens VA1	43.137	100	0.452
pilA/pilAI	Pseudomonas stutzeri DSM 10701	44.521	100	0.445
pilA	Vibrio cholerae C6706	39.189	100	0.397
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	39.189	100	0.397
pilA	Vibrio cholerae strain A1552	39.189	100	0.397
pilA	Acinetobacter baumannii strain A118	40.426	96.575	0.39
pilA	Pseudomonas aeruginosa PAK	36.364	100	0.384
pilA/pilAII	Pseudomonas stutzeri DSM 10701	39.716	96.575	0.384

Detailed information NTG0555904