Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J8380_RS06115 Genome accession   NZ_CP072800
Coordinates   1206675..1208384 (-) Length   569 a.a.
NCBI ID   WP_210229265.1    Uniprot ID   -
Organism   Candidatus Thiothrix anitrata isolate A52     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1201675..1213384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8380_RS06090 (J8380_06090) ubiE 1202798..1203550 (-) 753 WP_210229255.1 bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE -
  J8380_RS06095 (J8380_06095) - 1203627..1204013 (+) 387 WP_210229257.1 hypothetical protein -
  J8380_RS06100 (J8380_06100) - 1204109..1204408 (+) 300 WP_210229259.1 hypothetical protein -
  J8380_RS06105 (J8380_06105) - 1204427..1205302 (-) 876 WP_210229261.1 A24 family peptidase -
  J8380_RS06110 (J8380_06110) pilC 1205322..1206584 (-) 1263 WP_210229262.1 type II secretion system F family protein Machinery gene
  J8380_RS06115 (J8380_06115) pilB 1206675..1208384 (-) 1710 WP_210229265.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J8380_RS06120 (J8380_06120) tfpZ 1208696..1209466 (-) 771 WP_210229266.1 TfpX/TfpZ family type IV pilin accessory protein -
  J8380_RS06125 (J8380_06125) - 1209484..1210890 (-) 1407 WP_210229268.1 O-antigen ligase -
  J8380_RS06130 (J8380_06130) pilA2 1211017..1211457 (-) 441 WP_210230560.1 pilin Machinery gene
  J8380_RS06135 (J8380_06135) - 1211726..1212478 (+) 753 WP_210229270.1 ATP-binding protein -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62464.97 Da        Isoelectric Point: 6.8255

>NTDB_id=555903 J8380_RS06115 WP_210229265.1 1206675..1208384(-) (pilB) [Candidatus Thiothrix anitrata isolate A52]
MTPLNASTPLPGLLRQLVAQDAISAEQASQALQKSRIDKMPLVSWLAQSRFANTADIAYAASLEYGLSFVDIDQVNITPA
IANLLNTEQMQKLHLVPLYRNAKTLMVGLADPIHLTNLDDVKFATGLTPEPVFVEYDKLQRYLHAESGEITQPNPVTVAQ
QFTSAPAIVLDTRELKSKSDNEESSVVEFVDKLLAHAINTKASDIHIEIFEKSMRIRYRIDGILHVVATPPNDSAQQLIS
RLKVLARMDISERRVPQDGRIRFTLNAKKKIDFRVNTLPTLYGEKVVMRILDSSNATLGVEKLGFNPRQQQDFLKALQKP
DGMILVTGPTGSGKTVTLYTGLGILNTPERNISTAEDPAEINMPGVNQVNVNAKVGLTFADALRSFLRQDPDVIMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPPFNIASSVHLIVAQRLARRLCEKCKRPATNIPSEALLELG
FKAAEIPSLTIYEPCGCEACSHGYKGRVGLYQVMPISAEMGRMVMNGCNSIELADQALKEGIFDLRQSGLEKVRQGITSL
AELARVTTD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=555903 J8380_RS06115 WP_210229265.1 1206675..1208384(-) (pilB) [Candidatus Thiothrix anitrata isolate A52]
ATGACACCGCTTAACGCCTCCACTCCATTACCCGGCTTATTACGGCAATTAGTTGCTCAGGACGCTATTAGTGCTGAACA
AGCTAGCCAAGCTCTGCAAAAATCACGCATTGATAAAATGCCCCTAGTATCTTGGCTGGCACAATCACGTTTTGCCAACA
CTGCGGATATTGCTTACGCAGCCAGCCTAGAATATGGCCTAAGCTTTGTGGATATTGACCAAGTAAACATCACCCCCGCC
ATCGCTAACTTGTTGAATACCGAACAAATGCAAAAATTGCATTTAGTTCCATTGTACCGCAATGCAAAAACCCTAATGGT
AGGCTTGGCAGACCCCATCCACCTCACCAATTTAGATGATGTAAAATTTGCAACCGGTTTAACCCCCGAACCTGTTTTCG
TCGAATACGATAAATTACAGCGCTACCTCCATGCCGAGAGCGGAGAAATCACTCAACCCAATCCGGTGACGGTGGCACAA
CAATTCACCTCGGCTCCGGCGATTGTACTGGACACTCGCGAACTGAAAAGTAAAAGCGATAACGAAGAATCTTCAGTCGT
TGAATTCGTTGATAAGTTACTGGCGCACGCAATCAATACCAAAGCATCCGATATTCACATTGAAATTTTTGAAAAAAGCA
TGCGAATTCGCTACCGCATTGACGGCATCTTACACGTCGTGGCAACACCACCGAATGATTCGGCGCAACAGCTCATCTCA
CGACTCAAAGTATTGGCGCGGATGGATATTTCCGAACGGCGCGTACCGCAAGACGGGCGGATTCGTTTTACCCTCAATGC
CAAGAAAAAAATCGACTTCCGAGTTAATACCCTACCTACACTTTACGGCGAAAAAGTGGTTATGCGGATTCTCGATTCCT
CTAATGCCACCCTTGGTGTGGAAAAGCTAGGATTTAATCCACGTCAACAACAGGATTTCCTCAAAGCTCTGCAAAAACCT
GATGGCATGATTTTGGTCACGGGGCCGACGGGCAGTGGGAAAACAGTCACGCTGTACACCGGACTTGGCATCCTGAATAC
CCCGGAACGAAATATTTCCACAGCGGAAGACCCGGCTGAAATTAATATGCCCGGTGTTAATCAAGTCAATGTTAATGCCA
AAGTTGGGTTAACTTTCGCGGACGCGCTGCGCTCATTCTTACGTCAAGACCCGGATGTCATTATGGTCGGGGAAATTCGC
GACCTTGAAACCGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACTTGGTACTCTCAACGTTACACACCAATAGCGC
ACCGGAAACGCTGACGCGCTTGCTCAATATGGGCGTACCACCGTTTAACATTGCTTCATCCGTACATTTGATTGTAGCGC
AACGTCTGGCGCGACGACTCTGTGAAAAGTGCAAACGTCCGGCCACCAATATTCCCTCCGAAGCATTGCTTGAATTAGGA
TTTAAGGCCGCAGAAATTCCCAGCCTCACAATTTACGAACCCTGCGGCTGTGAGGCATGTTCCCACGGCTACAAAGGTCG
TGTTGGGCTTTACCAAGTCATGCCAATTTCGGCGGAAATGGGACGCATGGTAATGAATGGTTGTAACTCGATAGAATTAG
CGGATCAGGCACTCAAAGAAGGTATTTTCGATTTACGCCAATCGGGCTTGGAAAAAGTACGTCAGGGCATCACCTCTTTA
GCGGAGCTAGCACGCGTCACCACCGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-144)

Type II/IV secretion system protein (T2SSE)

(193-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

49.147

100

0.506
  pilB Acinetobacter baumannii D1279779

48.635

100

0.501
  pilB Legionella pneumophila strain ERS1305867

48.522

100

0.49
  pilF Neisseria gonorrhoeae MS11

48.221

98.77

0.476
  pilB Vibrio campbellii strain DS40M4

45.679

99.649

0.455
  pilB Vibrio parahaemolyticus RIMD 2210633

45.907

98.77

0.453
  pilB Vibrio cholerae strain A1552

48.833

90.334

0.441
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.647

97.715

0.397
  pilF Thermus thermophilus HB27

39.344

96.485

0.38