Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J8I93_RS17425 Genome accession   NZ_CP072689
Coordinates   3681816..3682796 (-) Length   326 a.a.
NCBI ID   WP_072274873.1    Uniprot ID   A0A370V2R0
Organism   Escherichia marmotae strain YF8     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3676816..3687796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8I93_RS17395 (J8I93_17205) - 3677490..3677987 (+) 498 WP_000856773.1 SprT family zinc-dependent metalloprotease -
  J8I93_RS17400 (J8I93_17210) endA 3678082..3678789 (+) 708 WP_000286521.1 deoxyribonuclease I -
  J8I93_RS17405 (J8I93_17215) rsmE 3678870..3679601 (+) 732 WP_001517055.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  J8I93_RS17410 (J8I93_17220) gshB 3679614..3680564 (+) 951 WP_000593301.1 glutathione synthase -
  J8I93_RS17415 (J8I93_17225) - 3680673..3681236 (+) 564 WP_010378695.1 YqgE/AlgH family protein -
  J8I93_RS17420 (J8I93_17230) ruvX 3681236..3681652 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  J8I93_RS17425 (J8I93_17235) pilT 3681816..3682796 (-) 981 WP_072274873.1 type IV pilus twitching motility protein PilT Machinery gene
  J8I93_RS17430 (J8I93_17240) yggS 3682814..3683518 (+) 705 WP_010378707.1 pyridoxal phosphate homeostasis protein -
  J8I93_RS17435 (J8I93_17245) yggT 3683536..3684102 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  J8I93_RS17440 (J8I93_17250) yggU 3684099..3684389 (+) 291 WP_001277217.1 DUF167 family protein YggU -
  J8I93_RS17445 (J8I93_17255) - 3684397..3684990 (+) 594 WP_001174757.1 XTP/dITP diphosphatase -
  J8I93_RS17450 (J8I93_17260) hemW 3684983..3686119 (+) 1137 WP_061090724.1 radical SAM family heme chaperone HemW -
  J8I93_RS17455 (J8I93_17265) - 3686118..3686561 (-) 444 Protein_3405 DUF1202 family protein -
  J8I93_RS17460 (J8I93_17270) ansB 3686678..3687724 (-) 1047 WP_000394192.1 L-asparaginase 2 -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36031.22 Da        Isoelectric Point: 5.7980

>NTDB_id=555167 J8I93_RS17425 WP_072274873.1 3681816..3682796(-) (pilT) [Escherichia marmotae strain YF8]
MNMEEIVTLSVKHNVSDLHLCSAWPARWRIRGRMEVAPFDASDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPAHCPQLEQLGAPPVLPELLNSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=555167 J8I93_RS17425 WP_072274873.1 3681816..3682796(-) (pilT) [Escherichia marmotae strain YF8]
ATGAATATGGAAGAAATTGTGACCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCAGCACG
ATGGCGCATTCGAGGGAGAATGGAGGTCGCGCCGTTTGACGCGTCAGACGTCGAAGAGTTGTTGCGGGAGTGGCTGGATG
ACGATCAACGGGCGATATTGCTTGAGAATGGCCAGCTTGATTTTGCGGTATCGCTGGCTGAAAACCAGCGGCTACGAGGC
AGTGCGTTTGCGCAGCGGCAAGGCATTTCTCTGGCGTTACGGTTGTTACCTGCGCATTGCCCGCAGCTCGAACAACTTGG
CGCGCCGCCGGTATTGCCGGAATTACTCAATAGCGAGAATGGCCTGATTCTGGTGACGGGAGCGACGGGGAGCGGCAAAT
CGACCACACTGGCGGCGATGGTTGGTTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGCTTGATCCAGCAGCGAGAGATTGGTTTGCACTGTATGACGTTTGCATCGGGATTACG
TGCGGCATTGCGGGAAGATCCCGATGTGATATTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACGCGCGGTGCAGCGCAGGCGGTCGAGCGGCTGGTAGATTCA
TTTCCGGCACAGGAAAAAGATCCCGTGCGTAATCAACTGGCCGGTAGTTTGCGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAGCAGGAAGGGCGCGTGGCGCTGTTTGAATTGTTGATTAACACGCCAGCGGTGGGGAATTTGATACGCGAAGGGA
AAACCCACCAGTTGCCACATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACTTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A370V2R0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilB Legionella pneumophila strain ERS1305867

31.414

100

0.368
  pilU Acinetobacter baylyi ADP1

37.037

99.387

0.368