Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J8Z27_RS01990 Genome accession   NZ_CP072680
Coordinates   420563..421600 (+) Length   345 a.a.
NCBI ID   WP_252035905.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43186     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 415563..426600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8Z27_RS01960 (J8Z27_01950) - 416337..416747 (-) 411 WP_252035901.1 hypothetical protein -
  J8Z27_RS01965 (J8Z27_01955) - 416758..417708 (-) 951 WP_252035902.1 hypothetical protein -
  J8Z27_RS01970 (J8Z27_01960) yggU 418091..418381 (-) 291 WP_252035903.1 DUF167 family protein YggU -
  J8Z27_RS01975 (J8Z27_01965) - 418381..418938 (-) 558 WP_239868989.1 YggT family protein -
  J8Z27_RS01980 (J8Z27_01970) proC 418977..419795 (-) 819 WP_239876883.1 pyrroline-5-carboxylate reductase -
  J8Z27_RS01985 (J8Z27_01975) - 419822..420538 (-) 717 WP_252035904.1 YggS family pyridoxal phosphate-dependent enzyme -
  J8Z27_RS01990 (J8Z27_01980) pilT 420563..421600 (+) 1038 WP_252035905.1 type IV pilus twitching motility protein PilT Machinery gene
  J8Z27_RS01995 (J8Z27_01985) pilU 421612..422718 (+) 1107 WP_252036591.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J8Z27_RS02000 (J8Z27_01990) ruvX 422828..423250 (-) 423 WP_239868972.1 Holliday junction resolvase RuvX -
  J8Z27_RS02005 (J8Z27_01995) - 423268..423831 (-) 564 WP_239876885.1 YqgE/AlgH family protein -
  J8Z27_RS02010 (J8Z27_02000) gshB 423888..424838 (-) 951 WP_252035906.1 glutathione synthase -
  J8Z27_RS02015 (J8Z27_02005) rsmE 424852..425583 (-) 732 WP_252035907.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  J8Z27_RS02020 (J8Z27_02010) endA 425680..426384 (-) 705 WP_252035908.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38053.41 Da        Isoelectric Point: 6.1891

>NTDB_id=555023 J8Z27_RS01990 WP_252035905.1 420563..421600(+) (pilT) [Vibrio sp. SCSIO 43186]
MDIAELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGVPAFNHSEVHRLVFDIMNDAQRSEFEERLEVDFSFELPDVGR
FRVNAFNQSRGCAAVFRTIPTDIPTLEQLDAPAIFESIANMEKGLILVTGPTGSGKSTTLAAMVDHINRHHNKHILTIED
PIEFVHTNNKCLINQREVHSSTHSFNNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKGMVRSMLSESLRAVIAQKLLKRIGGGRTACHEIMMATPAIRNLIREDKVAQMQSVIQTGAAHGMQTMEQHS
KQLIAQGLVDAEEVSKKIEVEQGLF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=555023 J8Z27_RS01990 WP_252035905.1 420563..421600(+) (pilT) [Vibrio sp. SCSIO 43186]
ATGGATATCGCTGAATTACTAGATTTTAGTGTAAAGCATAATGCTTCAGATCTACATCTTTCTGCTGGTGTATCTCCCAT
GGTACGCATTGATGGTGAAGTAAGAAAGCTTGGCGTACCAGCATTCAACCATTCTGAAGTGCATCGTTTGGTGTTCGACA
TTATGAATGATGCCCAGCGTAGTGAATTTGAAGAGCGTCTCGAAGTCGACTTCTCCTTTGAGCTGCCAGACGTTGGCCGT
TTCAGGGTTAATGCTTTTAATCAGTCACGAGGTTGTGCGGCGGTTTTCCGTACCATTCCAACCGATATCCCGACTTTAGA
GCAGTTGGATGCACCGGCCATCTTTGAAAGTATTGCTAACATGGAAAAAGGCCTGATTTTGGTCACAGGGCCAACGGGCT
CTGGTAAATCGACGACATTGGCAGCAATGGTGGATCATATTAATCGCCACCATAACAAGCATATCCTTACGATTGAAGAT
CCGATTGAGTTTGTGCATACCAATAACAAATGTCTGATCAACCAGCGCGAAGTTCATAGTAGTACCCATAGTTTTAATAA
CGCGCTGCGCAGTGCTCTACGTGAAGACCCTGATGTTATTCTAGTAGGCGAGTTACGTGATCAGGAAACGATCAGTCTAG
CGCTGACCGCTGCTGAAACCGGCCACTTGGTGTTTGGTACTTTGCACACCAGTTCGGCGGCGAAAACTATCGACCGTATT
ATTGATGTTTTTCCGGGCAGCGATAAAGGCATGGTGCGTTCCATGCTTTCTGAGTCTCTGCGTGCGGTGATCGCTCAGAA
ATTGCTCAAACGCATTGGCGGCGGGAGAACGGCTTGTCATGAAATCATGATGGCAACGCCTGCGATTCGTAACTTGATCC
GCGAAGATAAAGTGGCGCAAATGCAGTCTGTGATCCAGACAGGTGCAGCACATGGTATGCAGACCATGGAGCAGCACTCG
AAACAGTTGATTGCTCAAGGTCTGGTTGATGCAGAAGAAGTGTCGAAAAAAATTGAAGTTGAGCAAGGTCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

87.246

100

0.872
  pilT Vibrio cholerae strain A1552

87.246

100

0.872
  pilT Acinetobacter baylyi ADP1

69.006

99.13

0.684
  pilT Acinetobacter baumannii strain A118

69.578

96.232

0.67
  pilT Acinetobacter baumannii D1279779

69.578

96.232

0.67
  pilT Acinetobacter nosocomialis M2

69.277

96.232

0.667
  pilT Pseudomonas stutzeri DSM 10701

67.062

97.681

0.655
  pilT Pseudomonas aeruginosa PAK

65.789

99.13

0.652
  pilT Legionella pneumophila strain Lp02

67.173

95.362

0.641
  pilT Legionella pneumophila strain ERS1305867

67.173

95.362

0.641
  pilT Neisseria meningitidis 8013

64.497

97.971

0.632
  pilT Neisseria gonorrhoeae MS11

63.235

98.551

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.515

95.652

0.493
  pilU Vibrio cholerae strain A1552

41.846

94.203

0.394
  pilU Pseudomonas stutzeri DSM 10701

39.82

96.812

0.386
  pilU Acinetobacter baylyi ADP1

40.938

92.754

0.38