Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   J8Z27_RS01995 Genome accession   NZ_CP072680
Coordinates   421612..422718 (+) Length   368 a.a.
NCBI ID   WP_252036591.1    Uniprot ID   A0A9Q8XJR1
Organism   Vibrio sp. SCSIO 43186     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 416612..427718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8Z27_RS01965 (J8Z27_01955) - 416758..417708 (-) 951 WP_252035902.1 hypothetical protein -
  J8Z27_RS01970 (J8Z27_01960) yggU 418091..418381 (-) 291 WP_252035903.1 DUF167 family protein YggU -
  J8Z27_RS01975 (J8Z27_01965) - 418381..418938 (-) 558 WP_239868989.1 YggT family protein -
  J8Z27_RS01980 (J8Z27_01970) proC 418977..419795 (-) 819 WP_239876883.1 pyrroline-5-carboxylate reductase -
  J8Z27_RS01985 (J8Z27_01975) - 419822..420538 (-) 717 WP_252035904.1 YggS family pyridoxal phosphate-dependent enzyme -
  J8Z27_RS01990 (J8Z27_01980) pilT 420563..421600 (+) 1038 WP_252035905.1 type IV pilus twitching motility protein PilT Machinery gene
  J8Z27_RS01995 (J8Z27_01985) pilU 421612..422718 (+) 1107 WP_252036591.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J8Z27_RS02000 (J8Z27_01990) ruvX 422828..423250 (-) 423 WP_239868972.1 Holliday junction resolvase RuvX -
  J8Z27_RS02005 (J8Z27_01995) - 423268..423831 (-) 564 WP_239876885.1 YqgE/AlgH family protein -
  J8Z27_RS02010 (J8Z27_02000) gshB 423888..424838 (-) 951 WP_252035906.1 glutathione synthase -
  J8Z27_RS02015 (J8Z27_02005) rsmE 424852..425583 (-) 732 WP_252035907.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  J8Z27_RS02020 (J8Z27_02010) endA 425680..426384 (-) 705 WP_252035908.1 deoxyribonuclease I -
  J8Z27_RS02025 (J8Z27_02015) - 426463..426960 (-) 498 WP_239876888.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41200.83 Da        Isoelectric Point: 5.1885

>NTDB_id=555024 J8Z27_RS01995 WP_252036591.1 421612..422718(+) (pilU) [Vibrio sp. SCSIO 43186]
MDINAYLQGMLTEKASDLYITVGAPILYRVDGELKPHGESLSEAEVLTLLHSTMDEERQSEYSHSREANFAVVRDFGRFR
VSAFYQRELPGAVIRRIETTIPTFDDLKLPETLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNTERSGHILTVEDPI
EFVHEHQKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVIGQQLIRDKNGQGRHGVFEILLNSPRVSDLIQRGDLHELKSTMAKSQEMGMQTFDQALY
QLVVNDKITEEDALHSADSANDLRIMLKTQRGESFGGGALADVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=555024 J8Z27_RS01995 WP_252036591.1 421612..422718(+) (pilU) [Vibrio sp. SCSIO 43186]
ATGGATATCAATGCTTATCTGCAAGGCATGCTGACTGAGAAAGCGTCAGACTTATACATCACTGTTGGTGCGCCGATACT
TTATCGTGTTGATGGTGAGTTGAAGCCGCATGGAGAGAGCTTATCGGAAGCCGAAGTGTTGACGCTACTCCACAGCACCA
TGGATGAAGAGCGCCAGAGTGAGTATAGCCACAGTCGAGAAGCGAACTTTGCTGTTGTGCGAGATTTTGGTCGCTTTCGT
GTGTCCGCTTTCTACCAGCGAGAATTACCCGGTGCGGTTATTCGTAGAATTGAAACCACTATACCCACATTTGATGACTT
AAAACTGCCCGAAACTCTGAAAGATCTTTCCATCGCCAAGCGCGGTTTGGTGTTAGTGGTGGGTGCCACAGGTTCTGGTA
AATCCACAACAATGGCAGCGATGACGGGTTATCGAAATACTGAGCGCAGCGGCCATATTCTGACGGTGGAAGATCCGATT
GAGTTTGTTCATGAGCATCAGAAATGCATCGTCACGCAACGTGAGGTCGGCTTAGACACAGAAAGTTATGAAGTCGCACT
GAAGAACTCTTTGCGTCAGGCTCCTGATATGATCCTGATCGGTGAGATCCGATCGCGAGAGACCATGGAGTACGCGATGA
CCTTCGCCGAAACGGGCCATTTGTGTATGGCGACTTTGCACGCCAATAATGCTAACCAAGCTTTGGAAAGGATCCTGCAC
CTTGTACCGAAAGAGCAAAAAGAGCAGTTCTTGTTTGATCTCTCCATGAATCTTAAAGGTGTTATCGGCCAGCAGTTGAT
TCGAGATAAAAACGGCCAAGGTCGTCATGGCGTGTTTGAAATCTTACTCAACAGTCCACGAGTATCGGATCTCATTCAAC
GCGGTGATCTGCATGAACTCAAATCAACCATGGCGAAATCTCAGGAAATGGGTATGCAGACGTTTGATCAGGCGCTTTAC
CAACTGGTGGTGAATGACAAAATTACTGAAGAAGATGCGCTGCACAGTGCGGATTCAGCCAATGACCTGCGTATTATGCT
CAAGACTCAACGAGGTGAAAGCTTCGGTGGTGGTGCGCTGGCAGATGTGAAGATTGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.88

100

0.829
  pilU Pseudomonas stutzeri DSM 10701

57.429

95.109

0.546
  pilU Acinetobacter baylyi ADP1

52.991

95.38

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.953

92.12

0.405
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Acinetobacter baylyi ADP1

41.349

92.663

0.383
  pilT Acinetobacter baumannii D1279779

41.298

92.12

0.38
  pilT Acinetobacter baumannii strain A118

41.298

92.12

0.38
  pilT Acinetobacter nosocomialis M2

41.298

92.12

0.38
  pilT Pseudomonas aeruginosa PAK

41.003

92.12

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.538

88.315

0.367
  pilT Vibrio cholerae strain A1552

41.538

88.315

0.367
  pilT Neisseria meningitidis 8013

38.841

93.75

0.364
  pilT Neisseria gonorrhoeae MS11

38.841

93.75

0.364
  pilT Pseudomonas stutzeri DSM 10701

39.528

92.12

0.364