Detailed information    

experimental Experimentally validated

Overview

Name   recP/tkt   Type   Machinery gene
Locus tag   SP_RS07965 Genome accession   NC_003028
Coordinates   1515589..1517556 (-) Length   655 a.a.
NCBI ID   WP_000058257.1    Uniprot ID   A0A0H2XDS8
Organism   Streptococcus pneumoniae TIGR4     
Function   homologous recombination   
Homologous recombination

Function

Necessary for high-efficiency recombination chromosomal DNA during genetic transformation.


Genomic Context

Location: 1510589..1522556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP_RS07935 (SP_1609) - 1510867..1511664 (-) 798 WP_000881169.1 Nif3-like dinuclear metal center hexameric protein -
  SP_RS07940 (SP_1610) - 1511651..1512328 (-) 678 WP_000624092.1 tRNA (adenine(22)-N(1))-methyltransferase -
  SP_RS13460 (SP_1612) - 1512877..1513869 (+) 993 WP_000364830.1 protein kinase family protein -
  SP_RS12325 (SP_1613) - 1513943..1515214 (-) 1272 Protein_1519 IS3 family transposase -
  SP_RS07965 (SP_1615) recP/tkt 1515589..1517556 (-) 1968 WP_000058257.1 transketolase Machinery gene
  SP_RS07970 (SP_1616) alsE 1517593..1518288 (-) 696 WP_000086494.1 D-allulose 6-phosphate 3-epimerase -
  SP_RS07975 (SP_1617) - 1518299..1519384 (-) 1086 WP_000912151.1 PTS fructose transporter subunit IIC -
  SP_RS07980 (SP_1618) - 1519410..1519721 (-) 312 WP_000706301.1 PTS fructose transporter subunit IIB -
  SP_RS07985 (SP_1619) - 1519749..1520198 (-) 450 WP_000402984.1 fructose PTS transporter subunit IIA -
  SP_RS07990 (SP_1620) - 1520176..1520604 (-) 429 WP_001061751.1 PTS sugar transporter subunit IIA -
  SP_RS07995 (SP_1621) - 1520597..1522060 (-) 1464 WP_000087919.1 BglG family transcription antiterminator -

Sequence

Protein


Download         Length: 655 a.a.        Molecular weight: 70828.67 Da        Isoelectric Point: 4.9703

>NTDB_id=662 SP_RS07965 WP_000058257.1 1515589..1517556(-) (recP/tkt) [Streptococcus pneumoniae TIGR4]
MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLRITPEQPNWINRDRFILSAGHGSMLLYALLHLTGYKD
VSMDEIKNFRQWGSKTPGHPEVTHTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEG
VSAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRVRYDAYGWHTVLVEDGTDLAAISTAIETAKFSGKPSLIE
VKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLGWDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLVSDYKVAYPE
VASEIDAIVAGKSPVTITEKDFPVYENGFSQATRNSSQDAINTAAVLPTFLGGSADLAHSNMTYIKADGLQDKYNPLNRN
IQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAIRLSAIQELPVTYVFTHDSIAVGEDGPTHEPVEHLAGLRS
MPNLTVIRPADARETQAAWHHALTSTTTPTVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIKA
AKELVLQGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVIGIDIFGASAPAQTVIDN
YGFTVENIVAQVKSL

Nucleotide


Download         Length: 1968 bp        

>NTDB_id=662 SP_RS07965 WP_000058257.1 1515589..1517556(-) (recP/tkt) [Streptococcus pneumoniae TIGR4]
ATGTCACTACAATCAGTTAACGCCATTCGTTTTCTTGGCGTAGATGCTATTAACAAATCTAATTCTGGTCACCCGGGAAT
TGTCATGGGTGCTGCGCCAATGGCTTATAGCCTATTTACAAAGCACCTTAGAATTACACCTGAGCAGCCAAACTGGATTA
ACCGAGATCGCTTTATCTTGTCTGCGGGTCATGGATCAATGCTACTGTATGCTCTCTTGCATTTAACAGGGTATAAGGAT
GTATCCATGGACGAGATTAAAAATTTCCGGCAATGGGGATCTAAGACACCTGGTCATCCTGAAGTGACGCATACGTCTGG
TGTGGATGCGACATCTGGTCCGCTTGGTCAGGGGATTTCTACTGCCGTTGGTTTCGCCCAAGCAGAGCGTTTTTTAGCTG
CTAAGTACAACAAAGATGGTTTCCCTATTTTTGACCATTATACTTATGTTATCGCTGGAGACGGTGACTTCATGGAAGGA
GTGTCTGCGGAGGCGGCTTCTTATGCAGGTCATCAAGCTTTAGATAAGCTTATCGTCCTCTACGACTCCAACGACATCTG
CTTGGATGGTGAGACCAAAGATACTTTCTCTGAAAATGTTCGCGTCCGTTACGATGCTTATGGTTGGCATACAGTTCTGG
TAGAAGATGGAACAGATTTAGCAGCAATTTCTACAGCAATTGAGACGGCCAAGTTTTCTGGTAAACCGAGTTTGATTGAA
GTGAAAACGGTAATTGGTTACGGCTCACCCAATAAAAGTGGTACAAATGCTGTTCATGGTGCACCACTAGGAGCAGAAGA
AACAGGAGCAACTCGTAAGTTTTTGGGATGGGATTACGATCCATTTGAAGTACCAGAGGAAGTATATTCTGATTTCAAGA
CAAATGTAGCGGATCGTGGTCAGGAGGCATACGATGCTTGGGCTAGTTTGGTGTCTGATTACAAGGTTGCTTATCCCGAA
GTTGCTAGTGAGATTGACGCTATTGTAGCTGGAAAATCCCCTGTAACCATTACTGAAAAAGACTTCCCTGTCTATGAGAA
TGGCTTCTCTCAAGCAACTCGTAATTCGTCCCAAGATGCTATTAATACAGCAGCAGTTTTACCAACCTTCTTAGGTGGAT
CGGCAGACTTAGCTCACTCTAACATGACCTACATCAAGGCAGATGGCTTACAAGATAAATATAATCCATTAAACCGCAAT
ATTCAGTTTGGGGTACGTGAATTTGCCATGGGAACAATCCTCAATGGAATGGCTCTTCATGGTGGTTTACGAGTTTATGG
CGGAACCTTCTTTGTTTTCTCTGACTACGTCAAAGCTGCTATTCGGCTATCAGCCATTCAGGAGTTGCCTGTAACTTATG
TCTTTACCCATGATTCAATTGCCGTTGGTGAAGATGGTCCAACTCATGAACCAGTTGAACATTTGGCAGGTTTACGCTCA
ATGCCAAACTTGACTGTTATCCGTCCAGCGGATGCCCGTGAAACTCAAGCGGCTTGGCATCATGCCTTGACCAGTACCAC
CACTCCAACTGTCATTGTCTTAACCCGTCAAAACTTGGTAGTTGAAGAAGGGACAGACTTTGGTAAGGTCGCTAAAGGAG
CCTACGTCGTGTATGATACCCCGGGATTTGATACTATTATCATTGCTACAGGATCTGAGGTCAATCTAGCTATCAAAGCT
GCTAAGGAATTGGTTTTACAAGGTGGTAAAGTACGTGTGGTATCTATGCCCTCAACCGAACTATTTGATGCTCAAGATGC
TACCTACAAGGAAGACATTTTACCATCTAAGACTCGTCGTCGTGTGGCCATTGAAATGGCAGCGACCCAAAGTTGGTACA
AGTATGTTGGTTTGGATGGCGCGGTCATCGGTATTGACATCTTCGGTGCGTCTGCCCCAGCTCAGACTGTGATTGATAAT
TATGGATTTACGGTAGAGAATATCGTTGCTCAAGTTAAGTCCCTATAG

Domains

Predicted by InterProScan.

Transketolase, pyrimidine binding domain (Transket_pyr)

(352-519)

Transketolase, thiamine diphosphate binding domain (Transketolase_N)

(4-334)

Transketolase, C-terminal domain (Transketolase_C)

(535-648)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2XDS8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] J Reizer et al. (1993) A diverse transketolase family that includes the RecP protein of Streptococcus pneumoniae, a protein implicated in genetic recombination. Research in Microbiology 144(5):341-7. [PMID: 8248627]
[2] B A Radnis et al. (1990) Genetic transformation in Streptococcus pneumoniae: nucleotide sequence and predicted amino acid sequence of recP. Journal of Bacteriology 172(7):3669-74. [PMID: 2361942]
[3] D K Rhee et al. (1988) Genetic transformation in Streptococcus pneumoniae: molecular cloning and characterization of recP, a gene required for genetic recombination. Journal of Bacteriology 170(2):630-7. [PMID: 2828317]