Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J5226_RS23655 Genome accession   NZ_CP072597
Coordinates   5569862..5570899 (-) Length   345 a.a.
NCBI ID   WP_215837420.1    Uniprot ID   -
Organism   Lysobacter sp. K5869     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5564862..5575899
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5226_RS23650 (J5226_23650) pilU 5568579..5569745 (-) 1167 WP_215837418.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J5226_RS23655 (J5226_23655) pilT 5569862..5570899 (-) 1038 WP_215837420.1 type IV pilus twitching motility protein PilT Machinery gene
  J5226_RS23660 (J5226_23660) - 5571123..5571785 (+) 663 WP_255323137.1 YggS family pyridoxal phosphate-dependent enzyme -
  J5226_RS23665 (J5226_23665) - 5571782..5572339 (+) 558 WP_215837422.1 hypothetical protein -
  J5226_RS23670 (J5226_23670) proC 5572363..5573205 (+) 843 WP_215837424.1 pyrroline-5-carboxylate reductase -
  J5226_RS23675 (J5226_23675) - 5573202..5573663 (+) 462 WP_215837426.1 DUF4426 domain-containing protein -
  J5226_RS23680 (J5226_23680) - 5573785..5574789 (-) 1005 WP_215837428.1 lipase secretion chaperone -
  J5226_RS23685 (J5226_23685) - 5574792..5575736 (-) 945 WP_215837430.1 triacylglycerol lipase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38474.31 Da        Isoelectric Point: 6.7808

>NTDB_id=554472 J5226_RS23655 WP_215837420.1 5569862..5570899(-) (pilT) [Lysobacter sp. K5869]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDFEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIEQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYAHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGIPAIRNLIREDKVAQMYSAIQTGQGHGMMTLDQHL
QDLVKRGLILRPQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=554472 J5226_RS23655 WP_215837420.1 5569862..5570899(-) (pilT) [Lysobacter sp. K5869]
ATGGATATCGCCGAACTTCTGGCGTTTTCGGTAAAGAACAAAGCATCCGACTTGCATCTTTCGGCCGGCCTGCCGCCGAT
GATCCGGGTCGACGGCGACGTGCGCCGGATCAACATCCCGGCCCTGGACCACAAGCAGGTGCACGCCCTGGTGTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTTCGAAGAGTTCCTGGAAGTCGATTTCTCCTTCGAGATCCCGGGGCTGGCGCGT
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGTACCATTCCCTCCGAGGTGCTGACCCTCGA
GGATCTGGGCTGCCCGCCGATCTTCCGCCAGCTGATCGAACAGCCGCAGGGCCTGATCCTGGTGACCGGGCCGACCGGTT
CGGGCAAGTCGACCACGCTCGCGGCGATGATCGACCACATCAACAAGAACGAGTACGCGCACATCCTCTCGGTCGAAGAC
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTCCACCGCGACACGCACGGGTTCAACGA
GGCCCTGCGCTCGGCGCTGCGCGAAGACCCCGACTACATCCTCGTGGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACTTGGTGTTCGGCACCCTGCACACCTCCTCGGCGGCCAAGACGGTCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCGGAATCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTCAAGAAGGTCGGCGGCGGCCGTACCGCGGCCTGGGAAATCATGGTCGGCATCCCCGCGATCCGTAACCTCATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGGCGATCCAGACCGGCCAGGGCCACGGCATGATGACCCTGGACCAGCACCTG
CAAGATCTGGTCAAGCGCGGCCTGATCCTGCGCCCGCAGGCCAAGGAATACGCCAAGGACAAGCGTCTGTTCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

78.171

98.261

0.768
  pilT Legionella pneumophila strain Lp02

73.547

99.71

0.733
  pilT Legionella pneumophila strain ERS1305867

73.547

99.71

0.733
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.213

95.362

0.67
  pilT Vibrio cholerae strain A1552

70.213

95.362

0.67
  pilT Neisseria meningitidis 8013

65.789

99.13

0.652
  pilT Neisseria gonorrhoeae MS11

65.497

99.13

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Pseudomonas stutzeri DSM 10701

41.212

95.652

0.394
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391
  pilU Vibrio cholerae strain A1552

40.615

94.203

0.383