Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   J5226_RS23650 Genome accession   NZ_CP072597
Coordinates   5568579..5569745 (-) Length   388 a.a.
NCBI ID   WP_215837418.1    Uniprot ID   -
Organism   Lysobacter sp. K5869     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5563579..5574745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5226_RS23650 (J5226_23650) pilU 5568579..5569745 (-) 1167 WP_215837418.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J5226_RS23655 (J5226_23655) pilT 5569862..5570899 (-) 1038 WP_215837420.1 type IV pilus twitching motility protein PilT Machinery gene
  J5226_RS23660 (J5226_23660) - 5571123..5571785 (+) 663 WP_255323137.1 YggS family pyridoxal phosphate-dependent enzyme -
  J5226_RS23665 (J5226_23665) - 5571782..5572339 (+) 558 WP_215837422.1 hypothetical protein -
  J5226_RS23670 (J5226_23670) proC 5572363..5573205 (+) 843 WP_215837424.1 pyrroline-5-carboxylate reductase -
  J5226_RS23675 (J5226_23675) - 5573202..5573663 (+) 462 WP_215837426.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43109.59 Da        Isoelectric Point: 6.8287

>NTDB_id=554471 J5226_RS23650 WP_215837418.1 5568579..5569745(-) (pilU) [Lysobacter sp. K5869]
MNTPAPPNPTTAPGAIDFTSFLKLMAHQRASDLFITAGMPPSMKVHGKIAPITQNPLTPQQSRDLVLNVMNPQQREEFEK
THECNFAIGVTGVGRFRVSCFYQRNQVGMVLRRIETKIPTVEELNLPPIIKTLAMTKRGIIIFVGATGTGKSTSLAAMIG
YRNQNSTGHIITIEDPIEFVHRHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVL
CTLHANNANQAMDRIINFFPEDRRLQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEILLGTPLAQDYIRDGEIHKLKEL
MKESTQLGMKTFDQALFELYQGGEISYEDALRYADSQNEVRLRIKLAQGGDARTLSQGLDGVEVAEVR

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=554471 J5226_RS23650 WP_215837418.1 5568579..5569745(-) (pilU) [Lysobacter sp. K5869]
ATGAACACTCCCGCCCCGCCCAACCCGACCACCGCCCCCGGCGCGATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCA
CCAGCGCGCCTCCGACTTGTTCATCACCGCCGGCATGCCGCCGTCGATGAAGGTGCACGGCAAGATCGCCCCGATCACGC
AGAACCCGCTGACCCCGCAGCAGAGCCGCGACCTCGTGCTCAACGTGATGAACCCTCAGCAGCGCGAGGAGTTCGAGAAG
ACCCACGAGTGCAACTTCGCCATCGGCGTCACCGGCGTCGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAATCAGGT
CGGCATGGTGCTGCGCCGGATCGAGACCAAGATTCCGACCGTCGAAGAGCTGAACCTGCCGCCGATCATCAAGACGCTGG
CGATGACCAAGCGCGGCATCATCATCTTCGTCGGCGCCACCGGCACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGT
TACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGACCCGATCGAATTCGTGCACCGCCACGAGGGCTGCAT
CATCACCCAGCGCGAAGTCGGCATCGACACCGACAGCTGGGAAGCCGCGCTCAAGAACACCCTGCGTCAGGCGCCGGACG
TGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCATGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTG
TGCACGCTGCACGCCAACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCCTGCAGTT
GCTGATGGACTTGTCGCTGAACCTCAAGGGCGTGGTCGCCCAGCAGCTGATCCCGACCCCCGACGGCAAGGGCCGCCGCG
TGGCGATGGAAATCCTGCTCGGCACCCCGCTGGCGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGCTG
ATGAAGGAATCGACCCAGCTCGGCATGAAGACCTTCGATCAGGCGTTGTTCGAGCTCTACCAGGGCGGCGAGATCAGCTA
CGAAGACGCGCTGCGTTATGCGGACTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGACGCGCGGA
CCTTGTCGCAGGGGTTGGATGGGGTGGAAGTGGCCGAGGTGCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(130-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.482

94.588

0.657
  pilU Acinetobacter baylyi ADP1

64.706

92.01

0.595
  pilU Vibrio cholerae strain A1552

53.652

91.753

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.529

89.691

0.381