Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   THIMO_RS03300 Genome accession   NC_019940
Coordinates   748605..749642 (-) Length   345 a.a.
NCBI ID   WP_015279676.1    Uniprot ID   L0GW59
Organism   Thioflavicoccus mobilis 8321     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 743605..754642
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THIMO_RS03280 (Thimo_0686) - 744781..744966 (+) 186 WP_015279672.1 hypothetical protein -
  THIMO_RS18765 (Thimo_0687) - 745129..746472 (-) 1344 WP_015279673.1 TIGR00266 family protein -
  THIMO_RS03290 (Thimo_0688) - 746477..747199 (-) 723 WP_015279674.1 TIGR00266 family protein -
  THIMO_RS03295 (Thimo_0689) pilU 747428..748570 (-) 1143 WP_015279675.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  THIMO_RS03300 (Thimo_0690) pilT 748605..749642 (-) 1038 WP_015279676.1 type IV pilus twitching motility protein PilT Machinery gene
  THIMO_RS03305 (Thimo_0691) purE 750473..750973 (-) 501 WP_015279677.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  THIMO_RS03310 (Thimo_0692) purK 750970..752124 (-) 1155 WP_015279678.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  THIMO_RS18120 (Thimo_0693) - 752258..752752 (-) 495 WP_015279679.1 pilin -
  THIMO_RS03320 (Thimo_0694) - 752768..753403 (-) 636 WP_015279680.1 VWA domain-containing protein -
  THIMO_RS03325 (Thimo_0695) - 753409..754050 (-) 642 WP_015279681.1 VWA domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38310.00 Da        Isoelectric Point: 6.8360

>NTDB_id=55430 THIMO_RS03300 WP_015279676.1 748605..749642(-) (pilT) [Thioflavicoccus mobilis 8321]
MDIAELLAFSVKNNASDLHLSAGLPPMIRVDGDIRRINVPPLDHRTVHSMAYGIMNDKQRKDYEEFFETDFSFELPGMAR
FRVNAFNQNRGASVVFRTIPSEVLTLEDLDAPEIFRKIVDVPRGLVLVTGPTGSGKSTTLAAMVDYLNNKEYAHILTIED
PIEFVHVSKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTASETGHLVFATLHTSSAAKTIDRI
IDVFPAAEKTMVRSMLSESLRAVIAQTLLKKVGGGRIAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGQAHGMQTLDQCL
TDLVKRGIVGRAEARNRAQNKESFG

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=55430 THIMO_RS03300 WP_015279676.1 748605..749642(-) (pilT) [Thioflavicoccus mobilis 8321]
GTGGACATCGCCGAACTACTCGCCTTCAGCGTCAAGAACAACGCCTCGGATCTGCATCTGTCCGCCGGGCTTCCGCCGAT
GATCCGCGTCGACGGAGACATCCGCCGCATCAATGTCCCCCCCCTCGATCACCGGACGGTCCACTCGATGGCCTACGGGA
TCATGAACGACAAACAGCGCAAGGACTACGAGGAATTCTTCGAGACGGATTTCTCGTTCGAGCTCCCCGGCATGGCTCGG
TTCCGCGTCAACGCCTTCAATCAGAACCGCGGCGCCTCGGTGGTCTTCCGCACGATCCCGTCCGAAGTCCTGACGCTGGA
GGACCTCGACGCCCCGGAGATCTTCAGAAAGATCGTCGATGTGCCACGCGGCCTGGTGCTGGTGACCGGTCCCACGGGCT
CGGGCAAGTCGACGACCCTGGCGGCGATGGTGGACTACCTGAACAACAAGGAATATGCGCACATTCTCACGATCGAGGAC
CCGATCGAGTTCGTCCACGTCAGCAAGAAGTGCCTGGTCAATCAGCGCGAGGTACACCGCGACACACTCGGCTTCAGCGA
GGCCCTACGCTCGGCCCTGCGCGAGGACCCGGACATCATCCTGGTCGGCGAGATGCGGGATCTCGAGACCATCCGTCTGG
CCCTGACGGCCTCCGAAACGGGACACCTGGTCTTCGCCACCCTCCATACCAGCTCAGCGGCCAAGACCATCGACCGCATC
ATCGATGTCTTTCCCGCCGCCGAGAAGACGATGGTGCGCTCGATGCTCTCCGAATCGCTGCGCGCCGTCATCGCCCAAAC
GTTGCTGAAGAAGGTCGGAGGCGGCCGAATCGCCGCCCACGAGATCATGATCGGCACGCCGGCGATCCGCAACCTGATCC
GTGAGGACAAGGTCGCCCAGATGTACTCGTCGATCCAGACCGGCCAGGCCCATGGCATGCAGACCCTTGATCAGTGCCTC
ACCGACCTGGTCAAGAGGGGGATCGTCGGCCGTGCCGAGGCGCGCAACCGAGCCCAGAACAAGGAGTCGTTCGGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L0GW59

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

81.395

99.71

0.812
  pilT Pseudomonas aeruginosa PAK

80.233

99.71

0.8
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Acinetobacter baumannii strain A118

77.907

99.71

0.777
  pilT Acinetobacter nosocomialis M2

77.907

99.71

0.777
  pilT Acinetobacter baumannii D1279779

77.907

99.71

0.777
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.976

98.261

0.707
  pilT Vibrio cholerae strain A1552

71.976

98.261

0.707
  pilT Neisseria meningitidis 8013

67.826

100

0.678
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.675
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.49
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.101

0.406
  pilU Vibrio cholerae strain A1552

40.615

94.203

0.383
  pilU Acinetobacter baylyi ADP1

38.529

98.551

0.38

Multiple sequence alignment