Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   THIMO_RS03295 Genome accession   NC_019940
Coordinates   747428..748570 (-) Length   380 a.a.
NCBI ID   WP_015279675.1    Uniprot ID   L0GS12
Organism   Thioflavicoccus mobilis 8321     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 742428..753570
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THIMO_RS03275 (Thimo_0685) - 743042..744565 (-) 1524 WP_015279671.1 Ppx/GppA phosphatase family protein -
  THIMO_RS03280 (Thimo_0686) - 744781..744966 (+) 186 WP_015279672.1 hypothetical protein -
  THIMO_RS18765 (Thimo_0687) - 745129..746472 (-) 1344 WP_015279673.1 TIGR00266 family protein -
  THIMO_RS03290 (Thimo_0688) - 746477..747199 (-) 723 WP_015279674.1 TIGR00266 family protein -
  THIMO_RS03295 (Thimo_0689) pilU 747428..748570 (-) 1143 WP_015279675.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  THIMO_RS03300 (Thimo_0690) pilT 748605..749642 (-) 1038 WP_015279676.1 type IV pilus twitching motility protein PilT Machinery gene
  THIMO_RS03305 (Thimo_0691) purE 750473..750973 (-) 501 WP_015279677.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  THIMO_RS03310 (Thimo_0692) purK 750970..752124 (-) 1155 WP_015279678.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  THIMO_RS18120 (Thimo_0693) - 752258..752752 (-) 495 WP_015279679.1 pilin -
  THIMO_RS03320 (Thimo_0694) - 752768..753403 (-) 636 WP_015279680.1 VWA domain-containing protein -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42404.75 Da        Isoelectric Point: 6.5098

>NTDB_id=55429 THIMO_RS03295 WP_015279675.1 747428..748570(-) (pilU) [Thioflavicoccus mobilis 8321]
MDFRQLLALMMKKKASDLFITAGWPPSIKVDGTVHPIGNKPLTKAQASELVRGVMSDSQRLEFDKTNECQFAISAQDIGR
FRVSAFVQRNSPGMVLRRIETEIPTIEDLLLPPVLRDLAMTKRGIVFFVGGTGTGKSTSLAALVGYRNRHSTGHIITIED
PIEFMHQHDGCIVTQREVGVDTESFEVALKNTLRQAPDVILIGEIRTRQTMEYAIAFAETGHLVLATLHANNANQALDRI
INFFPEEGRRQLFMDLSLNLKGIVAQQLLPRKNGQGRRAAVEVLLNTPLASDLIRKGEVHKLKELMKRSKEQGMMTFDQA
LFDLYQEGEISYDDALKYADSANEVRLMIKLRGDNKTNLAEGMKDVAIVDDDGDDSPLQI

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=55429 THIMO_RS03295 WP_015279675.1 747428..748570(-) (pilU) [Thioflavicoccus mobilis 8321]
ATGGACTTCAGGCAATTGCTCGCGCTGATGATGAAGAAGAAGGCCTCCGACCTGTTCATTACGGCCGGCTGGCCGCCGAG
CATCAAGGTCGATGGCACCGTCCACCCGATCGGCAACAAGCCACTGACGAAGGCCCAGGCCAGCGAACTGGTGAGGGGCG
TGATGAGCGACAGCCAGCGCCTGGAGTTCGATAAGACCAACGAGTGCCAGTTCGCGATCAGCGCGCAAGACATCGGCCGC
TTCCGCGTCAGCGCCTTCGTCCAACGCAACTCCCCCGGCATGGTGCTGCGGCGCATCGAGACGGAGATCCCCACCATCGA
GGATCTGCTCCTGCCGCCGGTACTGCGCGACCTGGCGATGACCAAACGCGGTATCGTGTTCTTCGTGGGCGGCACCGGAA
CCGGCAAATCGACATCGCTCGCCGCCCTCGTCGGCTACCGCAATCGCCATAGCACCGGCCACATCATCACGATCGAAGAC
CCGATCGAGTTCATGCACCAACACGACGGCTGCATCGTCACGCAACGCGAGGTCGGGGTCGACACCGAGTCGTTCGAGGT
CGCGCTCAAGAACACGCTGCGCCAGGCCCCGGACGTGATCCTGATCGGCGAGATCCGCACCCGCCAGACGATGGAGTACG
CGATCGCCTTCGCCGAGACCGGCCACCTGGTCCTCGCGACCCTGCATGCCAACAACGCCAACCAGGCCCTCGACCGGATC
ATCAACTTCTTCCCCGAAGAGGGACGCCGGCAGTTGTTCATGGACCTGTCGCTGAACCTCAAGGGCATCGTCGCCCAGCA
ACTCCTACCGCGAAAGAACGGCCAGGGCCGGCGCGCCGCCGTCGAGGTGCTGCTCAACACGCCGCTCGCTTCGGACCTGA
TCCGCAAGGGTGAGGTCCACAAGCTCAAGGAGCTGATGAAACGCTCCAAGGAGCAAGGCATGATGACCTTCGACCAGGCG
CTGTTCGATCTCTACCAAGAAGGCGAGATCAGCTACGACGATGCCCTCAAGTATGCCGATTCGGCCAACGAGGTCCGTCT
GATGATCAAGTTGCGCGGCGACAACAAGACCAATCTCGCCGAAGGTATGAAAGACGTCGCCATCGTCGACGACGATGGCG
ACGACTCCCCGCTGCAGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L0GS12

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.085

98.947

0.674
  pilU Acinetobacter baylyi ADP1

63.315

96.842

0.613
  pilU Vibrio cholerae strain A1552

60.674

93.684

0.568
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.363

89.211

0.387
  pilT Legionella pneumophila strain Lp02

42.773

89.211

0.382
  pilT Legionella pneumophila strain ERS1305867

42.773

89.211

0.382
  pilT Pseudomonas stutzeri DSM 10701

41.298

89.211

0.368
  pilT Acinetobacter baumannii D1279779

42.296

87.105

0.368
  pilT Acinetobacter nosocomialis M2

42.296

87.105

0.368
  pilT Acinetobacter baumannii strain A118

42.296

87.105

0.368
  pilT Pseudomonas aeruginosa PAK

41.003

89.211

0.366
  pilT Vibrio cholerae strain A1552

41.818

86.842

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.818

86.842

0.363
  pilT Acinetobacter baylyi ADP1

40.469

89.737

0.363

Multiple sequence alignment