Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TVNIR_RS01395 Genome accession   NC_019902
Coordinates   292856..293890 (+) Length   344 a.a.
NCBI ID   WP_015257165.1    Uniprot ID   L0DSP8
Organism   Thioalkalivibrio nitratireducens DSM 14787     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 287856..298890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TVNIR_RS01365 (TVNIR_0292) hspQ 288195..288506 (+) 312 WP_015257158.1 heat shock protein HspQ -
  TVNIR_RS01370 (TVNIR_0293) - 288638..289300 (+) 663 WP_015257159.1 sulfite exporter TauE/SafE family protein -
  TVNIR_RS01375 (TVNIR_0294) - 289312..289764 (-) 453 WP_015257160.1 DUF4426 domain-containing protein -
  TVNIR_RS01380 (TVNIR_0295) - 290526..291083 (-) 558 WP_015257161.1 YggT family protein -
  TVNIR_RS01385 (TVNIR_0297) proC 291220..292038 (-) 819 WP_015257163.1 pyrroline-5-carboxylate reductase -
  TVNIR_RS01390 (TVNIR_0298) - 292035..292748 (-) 714 WP_015257164.1 YggS family pyridoxal phosphate-dependent enzyme -
  TVNIR_RS01395 (TVNIR_0299) pilT 292856..293890 (+) 1035 WP_015257165.1 type IV pilus twitching motility protein PilT Machinery gene
  TVNIR_RS01400 (TVNIR_0300) pilU 293952..295124 (+) 1173 WP_015257166.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TVNIR_RS01405 (TVNIR_0301) - 295132..296262 (+) 1131 WP_015257167.1 PilT/PilU family type 4a pilus ATPase -
  TVNIR_RS01410 (TVNIR_0302) - 296353..297645 (-) 1293 WP_015257168.1 dihydroorotase -
  TVNIR_RS01415 (TVNIR_0303) - 297642..298649 (-) 1008 WP_015257169.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38249.73 Da        Isoelectric Point: 6.6656

>NTDB_id=55309 TVNIR_RS01395 WP_015257165.1 292856..293890(+) (pilT) [Thioalkalivibrio nitratireducens DSM 14787]
MDITQLLSFAVKNGASDLHISAGMPPLVRIDGDMRRVNVPAMDHKQIQALIYDIMNDRQRKDYEEFWETDFSFELKGLAR
FRVNAFNQDRGAAAVFRTIPSKVLTLDDLGAPRVFEKISDYPRGIVLVTGPTGSGKSTTLAAMVNHKNDNEYGHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDTILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKDMVRAMLSESLRAVISQTLLKRVGGGRIAAHEIMLGTPAIRNLIRENKIAQMYSTIQTSANLGMQTLDQCL
KDMLAEGTITRDDARRRAVNPDAL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=55309 TVNIR_RS01395 WP_015257165.1 292856..293890(+) (pilT) [Thioalkalivibrio nitratireducens DSM 14787]
ATGGACATTACCCAGTTGCTTTCCTTTGCGGTCAAGAACGGCGCGTCGGACCTGCATATCTCGGCGGGCATGCCCCCGCT
GGTCCGCATCGATGGCGACATGCGTCGCGTGAATGTGCCGGCAATGGACCACAAACAGATCCAGGCGCTGATCTATGACA
TCATGAACGATCGTCAGCGCAAGGACTACGAGGAGTTCTGGGAAACCGACTTCTCGTTCGAGCTGAAGGGGCTTGCGCGG
TTTCGCGTCAATGCCTTCAACCAGGATCGGGGCGCGGCAGCGGTGTTCCGTACGATCCCGTCCAAGGTGCTGACGCTGGA
CGATCTGGGCGCGCCCCGGGTGTTCGAGAAGATCTCCGACTATCCGCGCGGCATCGTGCTGGTGACGGGGCCGACGGGGT
CCGGCAAGTCGACCACGCTCGCGGCGATGGTCAACCACAAGAACGACAACGAGTACGGCCACATCCTCACCATCGAGGAT
CCCATCGAGTTCGTGCACGAATCGAAGAAGTGCCTGGTGAACCAGCGCGAGGTACACCGGGACACGCACGGGTTCAGCGA
GGCCCTGCGCTCGGCGCTGCGCGAGGATCCGGACACGATCCTGGTCGGCGAGCTGCGCGACCTCGAAACGATTCGGCTGG
CGTTGACCGCGGCGGAAACCGGGCACCTGGTGTTCGGCACGCTGCACACCAGTTCCGCGGCCAAGACCATCGACCGGGTC
GTCGACGTGTTCCCGGCGGCGGAGAAGGACATGGTGCGGGCGATGCTGTCGGAATCCCTGCGCGCGGTGATCTCGCAAAC
GCTGCTGAAGCGCGTGGGCGGCGGCCGGATCGCTGCGCACGAGATCATGCTGGGGACGCCGGCGATCCGCAACCTGATTC
GCGAGAATAAGATCGCGCAAATGTATTCGACCATCCAGACGAGTGCCAACCTGGGCATGCAGACGCTCGACCAATGTCTG
AAGGACATGCTTGCGGAAGGCACGATAACGCGCGACGATGCAAGGCGTCGGGCGGTCAATCCCGACGCCCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L0DSP8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.24

99.419

0.788
  pilT Pseudomonas aeruginosa PAK

76.901

99.419

0.765
  pilT Acinetobacter baumannii D1279779

76.453

100

0.765
  pilT Acinetobacter baumannii strain A118

76.453

100

0.765
  pilT Acinetobacter nosocomialis M2

76.163

100

0.762
  pilT Acinetobacter baylyi ADP1

74.128

100

0.741
  pilT Legionella pneumophila strain Lp02

69.412

98.837

0.686
  pilT Legionella pneumophila strain ERS1305867

69.412

98.837

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Neisseria meningitidis 8013

64.348

100

0.645
  pilT Neisseria gonorrhoeae MS11

64.058

100

0.642
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.585

99.419

0.503
  pilU Acinetobacter baylyi ADP1

41.04

100

0.413
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

40.299

97.384

0.392

Multiple sequence alignment