Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   TVNIR_RS01400 Genome accession   NC_019902
Coordinates   293952..295124 (+) Length   390 a.a.
NCBI ID   WP_015257166.1    Uniprot ID   L0DUI9
Organism   Thioalkalivibrio nitratireducens DSM 14787     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 288952..300124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TVNIR_RS01375 (TVNIR_0294) - 289312..289764 (-) 453 WP_015257160.1 DUF4426 domain-containing protein -
  TVNIR_RS01380 (TVNIR_0295) - 290526..291083 (-) 558 WP_015257161.1 YggT family protein -
  TVNIR_RS01385 (TVNIR_0297) proC 291220..292038 (-) 819 WP_015257163.1 pyrroline-5-carboxylate reductase -
  TVNIR_RS01390 (TVNIR_0298) - 292035..292748 (-) 714 WP_015257164.1 YggS family pyridoxal phosphate-dependent enzyme -
  TVNIR_RS01395 (TVNIR_0299) pilT 292856..293890 (+) 1035 WP_015257165.1 type IV pilus twitching motility protein PilT Machinery gene
  TVNIR_RS01400 (TVNIR_0300) pilU 293952..295124 (+) 1173 WP_015257166.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TVNIR_RS01405 (TVNIR_0301) - 295132..296262 (+) 1131 WP_015257167.1 PilT/PilU family type 4a pilus ATPase -
  TVNIR_RS01410 (TVNIR_0302) - 296353..297645 (-) 1293 WP_015257168.1 dihydroorotase -
  TVNIR_RS01415 (TVNIR_0303) - 297642..298649 (-) 1008 WP_015257169.1 aspartate carbamoyltransferase catalytic subunit -
  TVNIR_RS01420 (TVNIR_0304) pyrR 298646..299152 (-) 507 WP_015257170.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  TVNIR_RS01425 (TVNIR_0305) ruvX 299145..299546 (-) 402 WP_015257171.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 390 a.a.        Molecular weight: 43686.02 Da        Isoelectric Point: 6.3521

>NTDB_id=55310 TVNIR_RS01400 WP_015257166.1 293952..295124(+) (pilU) [Thioalkalivibrio nitratireducens DSM 14787]
MDRDKAIRYMHDLLRTMVGRGGSDLFITVGMPPAAKINGLVQPMTDQKLTSAHTQVLVRSMMNDKQSAEFDRTHECNFAI
ALEGVSRFRVSAFTQRGSVGMVLRTIQGKIPSFEEINMPPQLKDIAMTRRGLVIFVGATSSGKSTSLAAMIGYRNRHAAD
HIITIEDPIEFVHPHDKSIVTQREVGVDTESYEVALKNTLRQAPDVILIGEIRDRATMDYGIAFAETGHLCLSTLHANNT
NQALDRIINFFPEERRPQLLMDLSLNLKAVVSQRLIRTQDGEGRVPAVEIMINTPLMSDLILKGAVHEMKDLIARSREQG
MQTFDQALFDLYETGKISYQEAIRNADSMNDLRLRVKLDSKRAQREEEPDVMDDLSGLSVADKADDFVAR

Nucleotide


Download         Length: 1173 bp        

>NTDB_id=55310 TVNIR_RS01400 WP_015257166.1 293952..295124(+) (pilU) [Thioalkalivibrio nitratireducens DSM 14787]
ATGGATCGTGACAAGGCCATACGTTACATGCACGACCTGCTGCGGACGATGGTGGGCCGCGGCGGGTCGGATCTGTTCAT
CACGGTGGGCATGCCGCCGGCGGCCAAGATCAATGGCCTGGTGCAGCCGATGACCGACCAGAAGCTGACCTCGGCCCATA
CGCAGGTGCTGGTGCGTTCGATGATGAACGACAAGCAGTCCGCCGAGTTCGACCGCACGCACGAGTGCAACTTCGCGATC
GCGCTCGAGGGGGTATCGCGCTTCCGCGTCAGTGCTTTTACCCAGCGCGGGAGCGTCGGCATGGTGCTGCGCACGATCCA
GGGCAAGATCCCGAGTTTCGAAGAGATCAACATGCCGCCGCAGCTCAAGGATATCGCGATGACCCGGCGCGGGCTCGTGA
TCTTCGTCGGGGCGACCTCGTCCGGCAAGTCGACCTCGCTGGCCGCGATGATCGGCTACCGCAACCGTCATGCGGCCGAT
CACATCATCACCATCGAGGATCCGATCGAGTTCGTGCATCCGCACGACAAGAGCATCGTGACCCAGCGCGAGGTCGGGGT
GGATACCGAAAGCTACGAGGTTGCGCTGAAAAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAGATCCGCG
ACCGGGCCACGATGGACTACGGCATCGCTTTCGCCGAAACCGGCCACCTGTGCCTGTCCACGCTACACGCGAACAACACC
AACCAGGCGCTGGATCGGATCATCAACTTCTTCCCGGAGGAGCGCCGGCCGCAGTTGCTGATGGACCTGTCGCTGAATCT
GAAGGCCGTTGTGTCGCAGCGCCTGATCCGGACACAGGATGGCGAAGGGCGGGTGCCGGCGGTGGAGATCATGATCAACA
CGCCGCTGATGTCCGACCTGATTCTGAAGGGGGCGGTGCACGAGATGAAGGACCTGATCGCCCGCTCGCGCGAGCAGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTCTACGAGACGGGCAAGATCAGTTACCAGGAAGCGATCCGCAACGCGGA
TTCGATGAACGATCTGCGTCTGCGGGTGAAGCTGGACAGCAAGCGGGCGCAGCGCGAGGAGGAACCCGACGTAATGGACG
ACCTCTCCGGCCTGTCGGTTGCCGACAAGGCCGACGATTTCGTCGCGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L0DUI9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.751

89.487

0.562
  pilU Acinetobacter baylyi ADP1

60

89.744

0.538
  pilU Vibrio cholerae strain A1552

55.172

89.231

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.687

85.897

0.367

Multiple sequence alignment