Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J1N51_RS00190 Genome accession   NZ_CP072110
Coordinates   39049..40113 (+) Length   354 a.a.
NCBI ID   WP_208832011.1    Uniprot ID   A0A975DCR2
Organism   Psychrosphaera ytuae strain MTZ26     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 34049..45113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1N51_RS00150 (J1N51_00150) rsfS 34543..34905 (-) 363 WP_208833266.1 ribosome silencing factor -
  J1N51_RS00155 (J1N51_00155) - 35024..35353 (-) 330 WP_208832004.1 DUF962 domain-containing protein -
  J1N51_RS00160 (J1N51_00160) - 35362..36012 (-) 651 WP_208832005.1 GNAT family N-acetyltransferase -
  J1N51_RS00165 (J1N51_00165) - 36150..36380 (+) 231 WP_208832006.1 DUF2789 domain-containing protein -
  J1N51_RS00170 (J1N51_00170) - 36430..36858 (-) 429 WP_208832007.1 DUF4426 domain-containing protein -
  J1N51_RS00175 (J1N51_00175) - 36890..37432 (-) 543 WP_208832008.1 YggT family protein -
  J1N51_RS00180 (J1N51_00180) proC 37434..38279 (-) 846 WP_208832009.1 pyrroline-5-carboxylate reductase -
  J1N51_RS00185 (J1N51_00185) - 38312..39013 (-) 702 WP_208832010.1 YggS family pyridoxal phosphate-dependent enzyme -
  J1N51_RS00190 (J1N51_00190) pilT 39049..40113 (+) 1065 WP_208832011.1 type IV pilus twitching motility protein PilT Machinery gene
  J1N51_RS00195 (J1N51_00195) pilU 40123..41247 (+) 1125 WP_208832012.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J1N51_RS00200 (J1N51_00200) rne 41322..44273 (-) 2952 WP_208832013.1 ribonuclease E -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39066.44 Da        Isoelectric Point: 5.5937

>NTDB_id=550423 J1N51_RS00190 WP_208832011.1 39049..40113(+) (pilT) [Psychrosphaera ytuae strain MTZ26]
MDITELLAFSVQHNASDLHLSAGTPPMIRVDGDVRRVNIPALDAKEVQSLIYDIMNDKQRKEYEENLETDFSFAVPDLAR
FRVNAFNMDRGAAAVFRTIPSEVLSLEDIGAPDIFREISENPRGLVLVTGPTGSGKSTTLAAMIDYINNQRHDHILTIED
PIEFVHQNKNSLISQREVHRDTKSFEAALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
VDVFPGDEKDMVRSMLSESLRAVISQTLCKKTGGGRVAAHEIMIGIPAIRNLIREDKIAQMYSSIQTGAAHGMQTMDQCL
LNLMNRGLISRDQALAKAHDKNVFSQGFGGGSGF

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=550423 J1N51_RS00190 WP_208832011.1 39049..40113(+) (pilT) [Psychrosphaera ytuae strain MTZ26]
ATGGATATTACAGAATTACTCGCATTTAGTGTGCAACATAATGCTTCAGATTTACACCTTTCTGCTGGCACACCGCCTAT
GATACGTGTAGACGGTGATGTACGTCGAGTCAACATTCCAGCTTTGGACGCAAAAGAAGTCCAGTCCTTGATTTACGACA
TCATGAACGACAAACAACGCAAAGAATACGAAGAAAACTTAGAAACAGACTTTTCTTTTGCGGTTCCTGACTTGGCTCGA
TTTCGTGTTAATGCATTTAACATGGATCGCGGTGCTGCAGCGGTATTTCGTACCATTCCATCAGAAGTGTTAAGTCTTGA
AGACATCGGCGCTCCTGATATTTTCCGAGAAATTTCAGAAAACCCTCGTGGTTTGGTTTTAGTAACCGGCCCAACAGGCT
CTGGTAAATCAACAACGCTTGCGGCGATGATTGACTATATTAATAACCAACGACACGATCATATTTTAACGATTGAAGAC
CCAATCGAATTTGTTCACCAGAACAAAAATAGCTTAATCAGCCAACGTGAAGTTCATCGCGATACTAAGAGCTTTGAAGC
AGCGCTTCGCTCGGCGTTACGTGAAGATCCAGATGTAATTCTCGTTGGTGAATTGCGTGATTTAGAAACAATTCGATTAG
CGATGACAGCTGCTGAAACAGGTCACTTAGTGTTTGGCACCTTGCACACCACTTCAGCACCAAAAACCATTGACCGTATT
GTTGACGTTTTCCCTGGTGACGAAAAAGATATGGTTCGCTCTATGTTATCCGAGTCATTACGCGCGGTTATTTCACAAAC
ATTGTGTAAGAAAACCGGTGGTGGACGAGTAGCAGCGCATGAGATCATGATTGGTATTCCAGCAATTCGAAACTTGATCC
GTGAAGATAAAATTGCGCAGATGTATTCTTCGATTCAAACCGGTGCAGCCCATGGTATGCAGACGATGGACCAGTGCTTG
TTAAACCTTATGAACAGAGGATTAATTAGCCGTGACCAAGCATTAGCCAAAGCGCACGATAAGAATGTATTTAGCCAAGG
GTTTGGCGGTGGTAGCGGGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.326

97.175

0.751
  pilT Pseudomonas stutzeri DSM 10701

76.453

97.175

0.743
  pilT Acinetobacter baumannii D1279779

75.872

97.175

0.737
  pilT Acinetobacter baumannii strain A118

75.872

97.175

0.737
  pilT Acinetobacter nosocomialis M2

75.872

97.175

0.737
  pilT Pseudomonas aeruginosa PAK

76.036

95.48

0.726
  pilT Legionella pneumophila strain Lp02

73.256

97.175

0.712
  pilT Legionella pneumophila strain ERS1305867

73.256

97.175

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.03

95.198

0.667
  pilT Vibrio cholerae strain A1552

70.03

95.198

0.667
  pilT Neisseria meningitidis 8013

67.919

97.74

0.664
  pilT Neisseria gonorrhoeae MS11

67.63

97.74

0.661
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.186

100

0.542
  pilU Pseudomonas stutzeri DSM 10701

41.108

96.893

0.398
  pilU Vibrio cholerae strain A1552

40.525

96.893

0.393
  pilU Acinetobacter baylyi ADP1

40.407

97.175

0.393