Detailed information NTG0550424

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: J1N51_RS00195
• Genome accession: NZ_CP072110
• Coordinates: 40123..41247 (+)
• Length: 374 a.a.
• NCBI ID: WP_208832012.1
• Uniprot ID: -
• Organism: Psychrosphaera ytuae strain MTZ26
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 35123..46247

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J1N51_RS00160 (J1N51_00160)	-	35362..36012 (-)	651	WP_208832005.1	GNAT family N-acetyltransferase	-
J1N51_RS00165 (J1N51_00165)	-	36150..36380 (+)	231	WP_208832006.1	DUF2789 domain-containing protein	-
J1N51_RS00170 (J1N51_00170)	-	36430..36858 (-)	429	WP_208832007.1	DUF4426 domain-containing protein	-
J1N51_RS00175 (J1N51_00175)	-	36890..37432 (-)	543	WP_208832008.1	YggT family protein	-
J1N51_RS00180 (J1N51_00180)	proC	37434..38279 (-)	846	WP_208832009.1	pyrroline-5-carboxylate reductase	-
J1N51_RS00185 (J1N51_00185)	-	38312..39013 (-)	702	WP_208832010.1	YggS family pyridoxal phosphate-dependent enzyme	-
J1N51_RS00190 (J1N51_00190)	pilT	39049..40113 (+)	1065	WP_208832011.1	type IV pilus twitching motility protein PilT	Machinery gene
J1N51_RS00195 (J1N51_00195)	pilU	40123..41247 (+)	1125	WP_208832012.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
J1N51_RS00200 (J1N51_00200)	rne	41322..44273 (-)	2952	WP_208832013.1	ribonuclease E	-
J1N51_RS00205 (J1N51_00205)	rluC	44810..45766 (+)	957	WP_208833267.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Sequence

Protein

• Length: 374 a.a.
• Molecular weight: 41657.95 Da
• Isoelectric Point: 6.0185

>NTDB_id=550424 J1N51_RS00195 WP_208832012.1 40123..41247(+) (pilU) [Psychrosphaera ytuae strain MTZ26]
MENIEQVFEKMVFEKASDLFITANLPISAKINGELTPLAPEVLDEMEAERLVRSCMSPKQQDEFDKTKECNFAVANEHGR
FRVSAFWQRDKVGMVVRRIVTTIPDVDELGLPPTLKDVIMAKRGLLLFVGGTGTGKSTSLAALLGYRNRNSKGHILTIED
PIEFVHEHKRCIVTQREVGIDTPSFESALKSSLRQAPDVILIGEIRSQEIMEYALSFAETGHLCVATLHANNANQAIDRI
MHLVPKEKHEKLKYDLALNLRAIVAQQLIQTSDGMGRVAAIEVLLNSPMVGELIKKGDISGIKEVMAKSKEMGMQTFDQA
LFELYQQSKISYADALHHADSPNDLRLMIKLRNNEQTGSGFLQGVTVDGLQKDD

Nucleotide

• Length: 1125 bp

>NTDB_id=550424 J1N51_RS00195 WP_208832012.1 40123..41247(+) (pilU) [Psychrosphaera ytuae strain MTZ26]
ATGGAAAATATAGAACAAGTGTTCGAAAAAATGGTGTTTGAGAAGGCATCTGATTTATTTATCACTGCAAACTTGCCAAT
CAGCGCCAAAATAAATGGTGAATTAACTCCGTTGGCACCAGAAGTTTTAGACGAAATGGAGGCTGAGCGGCTAGTTCGTA
GTTGTATGTCACCTAAACAACAAGACGAATTTGATAAAACCAAAGAATGTAATTTTGCGGTTGCTAACGAACACGGTCGA
TTTCGTGTGTCTGCGTTTTGGCAACGTGACAAAGTCGGTATGGTGGTTCGTCGTATCGTAACAACCATTCCTGATGTCGA
TGAGCTTGGCCTACCACCTACATTAAAAGATGTGATCATGGCAAAAAGAGGCCTGTTGCTGTTCGTTGGTGGCACGGGTA
CTGGTAAGTCGACAAGTTTGGCGGCATTACTCGGTTATCGCAATCGCAACTCGAAAGGTCATATCCTAACGATTGAAGAT
CCCATCGAGTTTGTACATGAACACAAGCGTTGTATTGTTACTCAACGTGAAGTTGGCATTGATACCCCGTCTTTTGAGTC
AGCACTGAAAAGCTCATTACGACAAGCACCAGATGTAATTTTGATTGGTGAGATTCGCTCTCAAGAGATCATGGAATACG
CCTTGTCATTTGCCGAAACAGGCCACCTTTGTGTTGCAACGTTACACGCAAATAATGCCAACCAAGCAATTGACCGTATC
ATGCACTTGGTACCAAAAGAAAAGCACGAAAAGTTAAAATATGATTTAGCACTCAACTTAAGAGCTATTGTTGCCCAACA
GTTGATTCAAACCAGTGATGGCATGGGCCGTGTTGCTGCGATTGAGGTTTTATTAAACTCGCCTATGGTTGGTGAGCTCA
TCAAGAAAGGCGATATCTCGGGTATTAAAGAGGTAATGGCTAAATCTAAAGAGATGGGTATGCAAACCTTCGATCAAGCA
TTGTTTGAGTTATACCAGCAATCTAAGATCAGTTATGCGGACGCACTACACCATGCAGACTCGCCAAATGACTTACGCTT
AATGATTAAGTTACGTAACAATGAACAGACGGGCTCTGGTTTCTTACAGGGTGTGACCGTCGATGGATTGCAAAAGGACG
ACTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (13-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	58.602	99.465	0.583
pilU	Pseudomonas stutzeri DSM 10701	57.105	100	0.58
pilU	Vibrio cholerae strain A1552	58.082	97.594	0.567
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.897	95.989	0.412