Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J4T76_RS09185 Genome accession   NZ_CP071867
Coordinates   2040183..2041127 (+) Length   314 a.a.
NCBI ID   WP_267341907.1    Uniprot ID   -
Organism   Gilliamella sp. B3022     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2035183..2046127
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4T76_RS09150 (J4T76_09180) ssb 2035279..2035800 (-) 522 WP_267341914.1 single-stranded DNA-binding protein Machinery gene
  J4T76_RS09155 (J4T76_09185) corA 2036028..2036969 (+) 942 WP_267341913.1 magnesium/cobalt transporter CorA -
  J4T76_RS09160 (J4T76_09190) - 2037164..2038123 (+) 960 WP_267341912.1 glucokinase -
  J4T76_RS09165 (J4T76_09195) - 2038143..2038391 (+) 249 WP_267341911.1 YcgL domain-containing protein -
  J4T76_RS09170 (J4T76_09200) - 2038388..2038810 (+) 423 WP_267341910.1 YcgN family cysteine cluster protein -
  J4T76_RS09175 (J4T76_09205) - 2038850..2039407 (-) 558 WP_267346237.1 YggT family protein -
  J4T76_RS09180 (J4T76_09210) - 2039445..2040146 (-) 702 WP_267341908.1 YggS family pyridoxal phosphate-dependent enzyme -
  J4T76_RS09185 (J4T76_09215) pilT 2040183..2041127 (+) 945 WP_267341907.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J4T76_RS09190 (J4T76_09220) proC 2041257..2042066 (+) 810 WP_267341906.1 pyrroline-5-carboxylate reductase -
  J4T76_RS09195 (J4T76_09225) - 2042373..2042582 (+) 210 WP_267341905.1 cold shock domain-containing protein -
  J4T76_RS09200 (J4T76_09230) - 2042737..2043006 (+) 270 WP_267346239.1 DUF5339 domain-containing protein -
  J4T76_RS09205 (J4T76_09235) - 2043024..2043509 (-) 486 WP_267346240.1 thermonuclease family protein -
  J4T76_RS09210 (J4T76_09240) fabA 2043783..2044304 (-) 522 WP_267341901.1 bifunctional 3-hydroxydecanoyl-ACP dehydratase/trans-2-decenoyl-ACP isomerase -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35158.60 Da        Isoelectric Point: 7.5381

>NTDB_id=548564 J4T76_RS09185 WP_267341907.1 2040183..2041127(+) (pilT) [Gilliamella sp. B3022]
MLNSMLALSVTQNASDLHLSSGEAPYVRINGQLQFLVVDKLIPNTLEMALFHLMNETQKKILKQYQQIDFAYHLPKVGRF
RVNIFYQHRGISAVFRVINHRIPTLSDIGAPAIFSELVKNDSGIILVTGSTGSGKSTTLAALIQYINITIAKHIITLEDP
IEYIYQNDKSLIQQREIADFGQTIDSLLRQDPDIILLGELRNKSSIQAALTAAETGHLVFATLHTNSAIQSINRIVDVFE
DHCRDFVRSQLANCLKAIISQKLICHNNERKALFEILINTPAISNLINEGKSKQILSLMQTGQQYGMQVMPDSF

Nucleotide


Download         Length: 945 bp        

>NTDB_id=548564 J4T76_RS09185 WP_267341907.1 2040183..2041127(+) (pilT) [Gilliamella sp. B3022]
ATGTTAAACTCAATGTTGGCACTTAGTGTAACGCAAAATGCGTCGGATTTGCACCTTTCAAGTGGTGAAGCGCCTTACGT
TCGTATTAACGGACAATTACAGTTTTTAGTTGTTGATAAGCTTATACCAAATACTCTTGAAATGGCATTATTTCATTTAA
TGAATGAAACCCAAAAAAAAATTCTGAAGCAATATCAGCAAATAGATTTTGCTTATCACTTGCCTAAAGTAGGGCGATTT
AGAGTGAATATTTTTTATCAGCACCGTGGTATTTCAGCCGTGTTTCGCGTGATCAATCATCGTATTCCTACGTTATCTGA
TATTGGTGCTCCTGCTATTTTTAGTGAGCTTGTAAAAAATGATTCAGGTATCATTCTTGTAACAGGATCAACGGGCAGTG
GTAAATCCACTACTTTAGCTGCTTTAATTCAATACATTAATATAACAATAGCGAAACACATTATTACGCTTGAAGATCCT
ATAGAATATATCTATCAAAATGATAAATCATTAATTCAGCAACGTGAAATAGCTGACTTTGGTCAAACCATTGATAGTTT
ATTAAGACAAGATCCGGATATCATTTTGTTAGGCGAATTACGCAATAAATCATCAATTCAAGCCGCATTGACTGCCGCAG
AAACAGGTCATTTGGTTTTTGCTACTTTGCATACTAACTCAGCGATACAAAGTATTAATCGAATTGTTGATGTTTTTGAA
GATCATTGTCGCGATTTTGTTCGCAGTCAACTTGCTAACTGCCTAAAAGCCATCATAAGCCAAAAGTTAATATGTCATAA
TAATGAACGTAAAGCCCTTTTTGAAATATTGATTAATACTCCTGCAATCAGTAATTTAATTAATGAAGGTAAGAGCAAAC
AAATTTTATCCCTTATGCAAACAGGCCAACAATATGGTATGCAAGTGATGCCAGACAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.804

99.045

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.804

99.045

0.503
  pilT Neisseria meningitidis 8013

47.962

100

0.487
  pilT Neisseria gonorrhoeae MS11

47.962

100

0.487
  pilT Acinetobacter nosocomialis M2

45.912

100

0.465
  pilT Acinetobacter baumannii D1279779

45.912

100

0.465
  pilT Acinetobacter baumannii strain A118

45.912

100

0.465
  pilT Legionella pneumophila strain Lp02

46.945

99.045

0.465
  pilT Legionella pneumophila strain ERS1305867

46.945

99.045

0.465
  pilT Pseudomonas stutzeri DSM 10701

45.86

100

0.459
  pilT Acinetobacter baylyi ADP1

44.969

100

0.455
  pilT Pseudomonas aeruginosa PAK

45.541

100

0.455
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.905

100

0.42
  pilU Acinetobacter baylyi ADP1

38.413

100

0.385
  pilU Pseudomonas stutzeri DSM 10701

36.656

99.045

0.363
  pilU Vibrio cholerae strain A1552

36.19

100

0.363