Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J4N42_RS12490 Genome accession   NZ_CP071859
Coordinates   2812929..2813966 (-) Length   345 a.a.
NCBI ID   WP_252028391.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43135     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2807929..2818966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N42_RS12460 (J4N42_12450) - 2808161..2808862 (+) 702 WP_252028378.1 endonuclease -
  J4N42_RS12465 (J4N42_12455) rsmE 2808987..2809718 (+) 732 WP_252028380.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  J4N42_RS12470 (J4N42_12460) gshB 2809731..2810678 (+) 948 WP_252028382.1 glutathione synthase -
  J4N42_RS12475 (J4N42_12465) - 2810726..2811289 (+) 564 WP_252028384.1 YqgE/AlgH family protein -
  J4N42_RS12480 (J4N42_12470) ruvX 2811322..2811744 (+) 423 WP_252028386.1 Holliday junction resolvase RuvX -
  J4N42_RS12485 (J4N42_12475) pilU 2811811..2812917 (-) 1107 WP_252028389.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J4N42_RS12490 (J4N42_12480) pilT 2812929..2813966 (-) 1038 WP_252028391.1 type IV pilus twitching motility protein PilT Machinery gene
  J4N42_RS12495 (J4N42_12485) - 2813992..2814702 (+) 711 WP_252028393.1 YggS family pyridoxal phosphate-dependent enzyme -
  J4N42_RS12500 (J4N42_12490) proC 2814746..2815564 (+) 819 WP_252028395.1 pyrroline-5-carboxylate reductase -
  J4N42_RS12505 (J4N42_12495) - 2815614..2816171 (+) 558 WP_252028396.1 YggT family protein -
  J4N42_RS12510 (J4N42_12500) yggU 2816171..2816461 (+) 291 WP_252028398.1 DUF167 family protein YggU -
  J4N42_RS12515 (J4N42_12505) - 2816534..2816965 (+) 432 WP_252028400.1 DUF4426 domain-containing protein -
  J4N42_RS12520 (J4N42_12510) - 2816988..2817584 (+) 597 WP_252028402.1 XTP/dITP diphosphatase -
  J4N42_RS12525 (J4N42_12515) hemW 2817603..2818772 (+) 1170 WP_252028404.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38371.95 Da        Isoelectric Point: 6.2714

>NTDB_id=548518 J4N42_RS12490 WP_252028391.1 2812929..2813966(-) (pilT) [Vibrio sp. SCSIO 43135]
MDIAELLDFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFSHADVHRLVFEIMNDAQRSEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPVEIPTLEQIDAPEIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINRHHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESMRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLVREDKVAQMYSIIQTGAAHGMQTMEQNA
KKLMAQGLVDVQEVEKKIEIESSPF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=548518 J4N42_RS12490 WP_252028391.1 2812929..2813966(-) (pilT) [Vibrio sp. SCSIO 43135]
ATGGATATCGCTGAGTTACTGGATTTTAGTGTAAAGCATAATGCTTCAGATCTACATCTTTCTGCTGGTGTACCGCCTAT
GGTACGTATAGATGGTGAAGTAAGAAAGCTTGGAGTACCAGCTTTTAGCCATGCCGATGTTCACCGTTTAGTTTTTGAGA
TCATGAATGACGCTCAACGTAGTGAGTTCGAGGAAAAGCTGGAAGTCGACTTTTCGTTTGAGTTGCCTAATGTGGGCCGT
TTTAGGGTGAATGCCTTTAACCAATCCCGTGGCTGCTCTGCCGTATTTCGAACCATTCCGGTTGAGATTCCTACCTTAGA
GCAGATCGATGCGCCAGAGATCTTTGAAAAAATTGCCAATTATGAAAAAGGGCTAGTGTTGGTCACGGGGCCTACGGGTT
CGGGTAAATCAACCACGCTGGCCGCGATGGTGGACTACATTAACCGTCACCACAATAAACACATTCTTACCATCGAAGAC
CCGATTGAGTTTGTTCATACCAACAATAAATGTTTGGTGAACCAACGTGAAGTACACAGAGATACCCATAGTTTCAAAAA
TGCGCTACGTAGCGCATTACGTGAGGATCCCGATGTTATTCTGGTAGGTGAGCTACGAGATCAAGAAACCATTAGCTTAG
CACTGACGGCTGCTGAAACAGGTCACTTAGTTTTTGGTACGCTGCATACCAGTTCAGCGGCGAAAACCATCGACCGTATT
ATTGATGTATTTCCGGGTAGTGATAAAGATATGGTGCGATCTATGTTGTCGGAGTCGATGCGTGCTGTGATTGCGCAGAA
GCTTCTCAAGCGTGTAGGTGGAGGCCGAGTCGCTTGTCATGAGATTATGCTAGCGACACCTGCGATTCGAAACCTTGTAC
GTGAAGACAAAGTGGCACAGATGTACTCCATCATTCAAACCGGTGCGGCTCACGGCATGCAAACGATGGAGCAAAATGCG
AAGAAATTAATGGCTCAAGGTCTTGTGGATGTCCAAGAAGTTGAGAAGAAAATAGAAATCGAGTCTTCACCGTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

91.304

100

0.913
  pilT Vibrio cholerae strain A1552

91.304

100

0.913
  pilT Acinetobacter baumannii D1279779

71.687

96.232

0.69
  pilT Acinetobacter baumannii strain A118

71.687

96.232

0.69
  pilT Acinetobacter nosocomialis M2

71.386

96.232

0.687
  pilT Acinetobacter baylyi ADP1

70.695

95.942

0.678
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Pseudomonas aeruginosa PAK

67.353

98.551

0.664
  pilT Legionella pneumophila strain Lp02

67.781

95.362

0.646
  pilT Legionella pneumophila strain ERS1305867

67.781

95.362

0.646
  pilT Neisseria meningitidis 8013

64.545

95.652

0.617
  pilT Neisseria gonorrhoeae MS11

62.353

98.551

0.614
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

42.216

96.812

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.419

96.812

0.391
  pilU Acinetobacter baylyi ADP1

39.062

92.754

0.362