Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   J4N42_RS12485 Genome accession   NZ_CP071859
Coordinates   2811811..2812917 (-) Length   368 a.a.
NCBI ID   WP_252028389.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43135     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2806811..2817917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N42_RS12455 (J4N42_12445) - 2807578..2808078 (+) 501 WP_252028376.1 SprT family zinc-dependent metalloprotease -
  J4N42_RS12460 (J4N42_12450) - 2808161..2808862 (+) 702 WP_252028378.1 endonuclease -
  J4N42_RS12465 (J4N42_12455) rsmE 2808987..2809718 (+) 732 WP_252028380.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  J4N42_RS12470 (J4N42_12460) gshB 2809731..2810678 (+) 948 WP_252028382.1 glutathione synthase -
  J4N42_RS12475 (J4N42_12465) - 2810726..2811289 (+) 564 WP_252028384.1 YqgE/AlgH family protein -
  J4N42_RS12480 (J4N42_12470) ruvX 2811322..2811744 (+) 423 WP_252028386.1 Holliday junction resolvase RuvX -
  J4N42_RS12485 (J4N42_12475) pilU 2811811..2812917 (-) 1107 WP_252028389.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J4N42_RS12490 (J4N42_12480) pilT 2812929..2813966 (-) 1038 WP_252028391.1 type IV pilus twitching motility protein PilT Machinery gene
  J4N42_RS12495 (J4N42_12485) - 2813992..2814702 (+) 711 WP_252028393.1 YggS family pyridoxal phosphate-dependent enzyme -
  J4N42_RS12500 (J4N42_12490) proC 2814746..2815564 (+) 819 WP_252028395.1 pyrroline-5-carboxylate reductase -
  J4N42_RS12505 (J4N42_12495) - 2815614..2816171 (+) 558 WP_252028396.1 YggT family protein -
  J4N42_RS12510 (J4N42_12500) yggU 2816171..2816461 (+) 291 WP_252028398.1 DUF167 family protein YggU -
  J4N42_RS12515 (J4N42_12505) - 2816534..2816965 (+) 432 WP_252028400.1 DUF4426 domain-containing protein -
  J4N42_RS12520 (J4N42_12510) - 2816988..2817584 (+) 597 WP_252028402.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41581.40 Da        Isoelectric Point: 6.1901

>NTDB_id=548517 J4N42_RS12485 WP_252028389.1 2811811..2812917(-) (pilU) [Vibrio sp. SCSIO 43135]
MDLNQCLQQMIELKASDLYLTVDAPILFRVDGELRAQGDKLNEGQVTRFLNAMMDDDRRESFKQTRESNFAVVRDSGRFR
VSAFFQRELPGAVIRRIETNIPTFEELKLPETLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHQHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFELLLNSPRVSDLIRRGDLHELKTTMAKSKEVGMQTFDQALY
QLVVEGKISEQDALHSADSANDLRLMLKTQRGESYDSGSLTNVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=548517 J4N42_RS12485 WP_252028389.1 2811811..2812917(-) (pilU) [Vibrio sp. SCSIO 43135]
ATGGATTTGAATCAGTGCTTACAACAGATGATCGAGCTTAAAGCATCGGATCTCTATTTAACGGTCGATGCCCCAATCCT
GTTCCGAGTAGACGGGGAACTCAGAGCTCAAGGAGACAAACTGAACGAGGGGCAGGTTACTCGCTTTCTCAATGCTATGA
TGGATGATGATCGTCGAGAAAGCTTTAAGCAAACTCGCGAATCTAATTTTGCCGTGGTTCGCGATAGTGGACGCTTTCGT
GTCAGTGCATTTTTTCAACGTGAGTTGCCGGGGGCGGTAATACGCCGCATTGAAACCAATATCCCAACCTTTGAAGAGCT
CAAATTACCTGAAACGCTGCAAGATTTGGCCATAGCTAAACGTGGGCTAGTGTTGGTTGTTGGAGCGACGGGCTCTGGTA
AATCTACGACGATGGCGGCAATGACGGGTTATCGTAATCAACATCGAACTGGGCATATTCTTACCGTTGAAGATCCCATT
GAGTTCGTGCACCAGCACCAGCGCTGCATCGTCACTCAGAGAGAGGTTGGGTTGGATACTGAAAGCTATGAAGTGGCGCT
TAAGAACTCTTTGCGCCAAGCTCCTGATATGATTCTAATTGGTGAGATCCGTAGTCGAGAGACGATGGAATACGCCATGA
CGTTTGCCGAAACGGGCCACCTATGTATGGCAACGCTGCATGCTAACAACGCCAATCAAGCGTTAGAACGGATTCTCCAC
CTTGTTCCTAAAGAGCAAAAAGAGCAGTTCTTGTTTGATTTGTCGATGAACCTAAAAGGGGTAGTAGGGCAGCAACTTAT
CCGCGACAAGAATGGCCAAGGTCGTCATGGGGTGTTTGAATTATTGCTAAACAGTCCTCGAGTCTCTGATTTAATTCGCC
GGGGTGATCTACATGAATTAAAAACGACGATGGCTAAATCGAAAGAGGTGGGTATGCAGACGTTTGATCAAGCGCTCTAT
CAGCTGGTGGTCGAGGGTAAAATCAGTGAACAAGATGCGTTGCACAGTGCTGACTCAGCAAATGATTTACGTCTGATGTT
AAAGACTCAGCGTGGTGAGAGCTACGATTCTGGCTCACTCACCAATGTGAAGATAGATATGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

86.141

100

0.861
  pilU Pseudomonas stutzeri DSM 10701

56.438

99.185

0.56
  pilU Acinetobacter baylyi ADP1

52.991

95.38

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.827

88.043

0.386
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Acinetobacter baylyi ADP1

41.056

92.663

0.38
  pilT Pseudomonas aeruginosa PAK

41.003

92.12

0.378
  pilT Vibrio cholerae strain A1552

40.719

90.761

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.761

0.37
  pilT Pseudomonas stutzeri DSM 10701

39.823

92.12

0.367