Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J4N45_RS02030 Genome accession   NZ_CP071850
Coordinates   383415..384455 (-) Length   346 a.a.
NCBI ID   WP_252013313.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43140     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 378415..389455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N45_RS01995 (J4N45_01990) - 379877..381499 (+) 1623 WP_252013309.1 methyl-accepting chemotaxis protein -
  J4N45_RS02025 (J4N45_02020) pilU 382296..383405 (-) 1110 WP_252013311.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J4N45_RS02030 (J4N45_02025) pilT 383415..384455 (-) 1041 WP_252013313.1 type IV pilus twitching motility protein PilT Machinery gene
  J4N45_RS02035 (J4N45_02030) - 384481..385191 (+) 711 WP_252013315.1 YggS family pyridoxal phosphate-dependent enzyme -
  J4N45_RS02040 (J4N45_02035) proC 385230..386048 (+) 819 WP_252013317.1 pyrroline-5-carboxylate reductase -
  J4N45_RS02045 (J4N45_02040) - 386080..386637 (+) 558 WP_128650533.1 YggT family protein -
  J4N45_RS02050 (J4N45_02045) yggU 386637..386924 (+) 288 WP_042475818.1 DUF167 family protein YggU -
  J4N45_RS02055 (J4N45_02050) - 386949..387380 (+) 432 WP_252013319.1 DUF4426 domain-containing protein -
  J4N45_RS02060 (J4N45_02055) - 387396..387998 (+) 603 WP_252013321.1 XTP/dITP diphosphatase -
  J4N45_RS02065 (J4N45_02060) hemW 388002..389171 (+) 1170 WP_252013323.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38681.32 Da        Isoelectric Point: 6.3408

>NTDB_id=548314 J4N45_RS02030 WP_252013313.1 383415..384455(-) (pilT) [Vibrio sp. SCSIO 43140]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLEIPSLEHSDVHRLIFDIMNDKQRSEYEEHLEIDFSFELPNIGR
FRVNAFNQSRGCAAVFRNIPTSPPTLEEIDAPRKFAELANLENGLVLVTGPTGSGKSTTLAAMVNHINEFQNKHILTIED
PIEFVHRNKKCLVNQREVHRDTHSFKAALRSALREDPDIILVGELRDHETISLALTAAETGHLVFGTMHTNSAAKSIDRI
IDVFPAQEKGMVRSMLSESLRAVIAQTLLKRLGGGRVACHEIMMGTPAIRNLIREDKVAQMFSVIQTGAAHGMQTMEQNA
QQLLSQGLVDVEEINKRVFIQQPMPM

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=548314 J4N45_RS02030 WP_252013313.1 383415..384455(-) (pilT) [Vibrio sp. SCSIO 43140]
ATGGATATCACCGAGTTACTAGACTTCAGTGTAAAGCATAATGCATCAGATCTACACCTTTCTGCTGGGGTTTCCCCCAT
GGTTAGAATTGATGGCGAAGTCAGAAAACTGGAGATACCATCACTCGAACATTCTGATGTGCACCGGCTCATCTTTGACA
TCATGAATGACAAGCAGCGCAGTGAATATGAAGAACATCTAGAAATCGACTTCTCCTTTGAACTCCCGAACATTGGTCGC
TTTCGCGTTAATGCATTTAATCAATCCCGCGGTTGTGCTGCCGTGTTTCGAAATATACCCACATCCCCTCCAACACTCGA
AGAGATTGACGCCCCAAGAAAGTTTGCCGAATTAGCGAACCTCGAAAATGGATTAGTGCTTGTGACAGGTCCTACGGGCT
CAGGAAAATCCACCACGCTTGCAGCAATGGTAAATCACATCAACGAGTTTCAAAACAAGCACATCCTTACTATTGAAGAC
CCCATTGAGTTTGTGCATCGCAATAAGAAATGCCTAGTCAATCAACGAGAAGTACACCGAGATACCCATAGCTTTAAAGC
AGCTCTCAGATCGGCGCTTAGAGAAGATCCTGACATCATCCTAGTGGGTGAATTAAGAGACCATGAAACCATCAGTCTCG
CCCTGACCGCGGCCGAGACAGGGCACTTAGTGTTCGGCACGATGCACACGAATTCCGCTGCAAAGTCTATTGACCGAATC
ATTGACGTTTTCCCGGCACAGGAGAAAGGTATGGTCAGATCTATGCTGTCTGAATCATTAAGAGCGGTGATTGCCCAAAC
ACTGCTAAAACGCCTCGGTGGTGGCCGGGTAGCATGTCACGAGATTATGATGGGAACCCCTGCGATCCGGAATCTAATAC
GTGAAGACAAGGTAGCTCAAATGTTTTCGGTCATTCAAACTGGCGCTGCGCACGGCATGCAAACGATGGAGCAGAATGCT
CAGCAACTCTTATCACAAGGGTTGGTCGATGTTGAGGAGATAAATAAAAGAGTCTTCATACAACAACCGATGCCTATGTA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

81.065

97.688

0.792
  pilT Vibrio cholerae strain A1552

81.065

97.688

0.792
  pilT Acinetobacter nosocomialis M2

69.679

99.133

0.691
  pilT Acinetobacter baumannii D1279779

69.388

99.133

0.688
  pilT Acinetobacter baumannii strain A118

69.388

99.133

0.688
  pilT Acinetobacter baylyi ADP1

70.997

95.665

0.679
  pilT Pseudomonas aeruginosa PAK

67.836

98.844

0.671
  pilT Pseudomonas stutzeri DSM 10701

67.347

99.133

0.668
  pilT Legionella pneumophila strain Lp02

69.301

95.087

0.659
  pilT Legionella pneumophila strain ERS1305867

69.301

95.087

0.659
  pilT Neisseria meningitidis 8013

63.905

97.688

0.624
  pilT Neisseria gonorrhoeae MS11

63.609

97.688

0.621
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.898

96.532

0.491
  pilU Vibrio cholerae strain A1552

41.846

93.931

0.393
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.532

0.387
  pilU Acinetobacter baylyi ADP1

40.312

92.486

0.373