Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   J4N45_RS02025 Genome accession   NZ_CP071850
Coordinates   382296..383405 (-) Length   369 a.a.
NCBI ID   WP_252013311.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43140     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 377296..388405
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N45_RS01995 (J4N45_01990) - 379877..381499 (+) 1623 WP_252013309.1 methyl-accepting chemotaxis protein -
  J4N45_RS02025 (J4N45_02020) pilU 382296..383405 (-) 1110 WP_252013311.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J4N45_RS02030 (J4N45_02025) pilT 383415..384455 (-) 1041 WP_252013313.1 type IV pilus twitching motility protein PilT Machinery gene
  J4N45_RS02035 (J4N45_02030) - 384481..385191 (+) 711 WP_252013315.1 YggS family pyridoxal phosphate-dependent enzyme -
  J4N45_RS02040 (J4N45_02035) proC 385230..386048 (+) 819 WP_252013317.1 pyrroline-5-carboxylate reductase -
  J4N45_RS02045 (J4N45_02040) - 386080..386637 (+) 558 WP_128650533.1 YggT family protein -
  J4N45_RS02050 (J4N45_02045) yggU 386637..386924 (+) 288 WP_042475818.1 DUF167 family protein YggU -
  J4N45_RS02055 (J4N45_02050) - 386949..387380 (+) 432 WP_252013319.1 DUF4426 domain-containing protein -
  J4N45_RS02060 (J4N45_02055) - 387396..387998 (+) 603 WP_252013321.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41434.41 Da        Isoelectric Point: 7.1056

>NTDB_id=548313 J4N45_RS02025 WP_252013311.1 382296..383405(-) (pilU) [Vibrio sp. SCSIO 43140]
MDTMDNILKQMLVCRASDLYLTADAPVLFRVDGDLKPHGSALHHHDVHSLLQEVMDSSSYQEYQASKEANFAIVKTYGRF
RVSAFFQRELPGAVIRRIETHIPSFEELALPDVVQDLAIAKRGLVLIVGGTGSGKSTTMAAMTGYRNRHRSGHILTVEDP
IEFVHQHQKCIVTQREVGLDTESYDIALKNSLRQAPDMIVIGEIRNRSTMEYAMAFAETGHLCMATLHANNANQALERIL
HLVPKERKEQFLFDLSANLRGIVAQQLIRDKHAQGRHGVFEVMLSSPRISDLIRKGALHELKAAMTKSNDVGMQTFDQAL
YQLVRAGKINQEDALHSADSQNDLRLMLKIQESESQEKNPLRGIKIDMS

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=548313 J4N45_RS02025 WP_252013311.1 382296..383405(-) (pilU) [Vibrio sp. SCSIO 43140]
ATGGATACGATGGATAATATTTTGAAGCAGATGCTCGTGTGTAGAGCGTCCGATCTTTATTTAACCGCGGATGCCCCCGT
CTTATTTAGAGTGGATGGAGATCTAAAGCCGCACGGCTCAGCCCTGCATCACCACGATGTTCACTCACTGCTACAAGAGG
TAATGGATAGTTCGAGTTATCAAGAATACCAGGCAAGTAAAGAAGCAAATTTTGCCATTGTTAAGACTTATGGGCGTTTT
CGAGTTAGTGCGTTCTTTCAAAGAGAGCTGCCAGGTGCGGTCATAAGGCGTATCGAAACTCACATTCCCAGTTTTGAAGA
ACTCGCACTTCCTGATGTAGTACAAGATCTTGCTATCGCCAAGCGTGGGCTAGTGCTGATCGTTGGAGGAACTGGCTCTG
GTAAGTCTACAACTATGGCGGCAATGACAGGTTACAGGAACCGCCACCGCAGTGGACATATACTGACGGTGGAGGACCCT
ATCGAGTTCGTCCACCAACATCAAAAATGCATTGTTACCCAGAGGGAAGTAGGGCTAGACACTGAAAGCTACGACATCGC
ACTCAAAAACTCTCTTCGACAAGCACCAGATATGATCGTCATTGGCGAGATTCGTAACCGTTCAACCATGGAGTACGCAA
TGGCTTTTGCCGAAACGGGTCACCTATGTATGGCAACACTACATGCGAACAACGCGAATCAAGCCTTAGAGAGGATACTT
CACTTAGTGCCAAAAGAGCGTAAGGAGCAGTTCCTGTTTGATCTCTCTGCGAACCTCAGAGGCATTGTCGCTCAGCAGCT
TATTAGAGATAAACATGCACAAGGTAGGCATGGGGTTTTTGAGGTTATGTTGAGCAGTCCAAGAATCTCAGACCTTATCC
GCAAAGGCGCCCTGCACGAGCTCAAAGCAGCAATGACGAAAAGTAATGATGTTGGAATGCAAACATTTGATCAGGCGCTA
TATCAATTGGTTAGGGCAGGAAAGATCAATCAAGAAGACGCTTTGCACAGTGCAGACTCTCAAAATGATCTCAGGCTAAT
GCTAAAGATTCAAGAAAGTGAATCTCAAGAGAAAAATCCGCTTAGGGGGATTAAGATTGATATGAGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

72.055

98.916

0.713
  pilU Pseudomonas stutzeri DSM 10701

54.054

100

0.542
  pilU Acinetobacter baylyi ADP1

48.876

96.477

0.472
  pilT Legionella pneumophila strain Lp02

42.638

88.347

0.377
  pilT Legionella pneumophila strain ERS1305867

42.638

88.347

0.377
  pilT Acinetobacter baylyi ADP1

41.768

88.889

0.371
  pilT Acinetobacter nosocomialis M2

41.231

88.076

0.363
  pilT Acinetobacter baumannii strain A118

41.231

88.076

0.363
  pilT Acinetobacter baumannii D1279779

41.231

88.076

0.363
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.923

88.076

0.36
  pilT Pseudomonas aeruginosa PAK

40.923

88.076

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.562

86.721

0.36
  pilT Vibrio cholerae strain A1552

41.562

86.721

0.36