Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   J4N39_RS01945 Genome accession   NZ_CP071848
Coordinates   377843..378952 (+) Length   369 a.a.
NCBI ID   WP_252021437.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43136     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 372843..383952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N39_RS01905 (J4N39_01905) - 373096..373539 (-) 444 WP_252021421.1 DUF4426 domain-containing protein -
  J4N39_RS01910 (J4N39_01910) - 373574..373885 (-) 312 WP_252021423.1 helix-turn-helix domain-containing protein -
  J4N39_RS01915 (J4N39_01915) - 373878..374225 (-) 348 WP_252021425.1 type II toxin-antitoxin system RelE/ParE family toxin -
  J4N39_RS01920 (J4N39_01920) yggU 374332..374622 (-) 291 WP_252021427.1 DUF167 family protein YggU -
  J4N39_RS01925 (J4N39_01925) - 374622..375179 (-) 558 WP_252021429.1 YggT family protein -
  J4N39_RS01930 (J4N39_01930) proC 375214..376032 (-) 819 WP_252021431.1 pyrroline-5-carboxylate reductase -
  J4N39_RS01935 (J4N39_01935) - 376057..376761 (-) 705 WP_252021433.1 YggS family pyridoxal phosphate-dependent enzyme -
  J4N39_RS01940 (J4N39_01940) pilT 376786..377832 (+) 1047 WP_252021435.1 type IV pilus twitching motility protein PilT Machinery gene
  J4N39_RS01945 (J4N39_01945) pilU 377843..378952 (+) 1110 WP_252021437.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J4N39_RS01950 (J4N39_01950) ruvX 379049..379474 (-) 426 WP_252021438.1 Holliday junction resolvase RuvX -
  J4N39_RS01955 (J4N39_01955) - 379692..380255 (-) 564 WP_252021439.1 YqgE/AlgH family protein -
  J4N39_RS01960 (J4N39_01960) gshB 380280..381227 (-) 948 WP_252021440.1 glutathione synthase -
  J4N39_RS01965 (J4N39_01965) rsmE 381240..381971 (-) 732 WP_252021441.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  J4N39_RS01970 (J4N39_01970) - 382113..382823 (-) 711 WP_252021442.1 endonuclease -
  J4N39_RS01975 (J4N39_01975) - 382902..383399 (-) 498 WP_252021443.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40957.79 Da        Isoelectric Point: 6.6926

>NTDB_id=548278 J4N39_RS01945 WP_252021437.1 377843..378952(+) (pilU) [Vibrio sp. SCSIO 43136]
MELNQILQQMVAKKASDIYITVGAPCLLRVHGELEAVGEVLNEHQVHTLLDSAMDDVRRGEFRRDKEANFAIVRDSGRYR
VSAFFQRELPGAVIRRIETQIPTFDELKLPGVLQDLSVAKRGLVLVVGATGSGKSTTLAAMTGHRNSTRTGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDVILIGEIRSRETMQYAMTFAETGHLCMATLHANNANQAIERILH
LVPKEQKEQFLFDLSMNLKGVIAQQLIRDKSGQGRHGVFEILLNTPRVADLIRRGDLHELKTTMAKSSQSGMQTFDQSLY
ELISAGKITEQDGLHSADSANDLRMMLKSKQSGTSSSSGLLDGVQIDMD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=548278 J4N39_RS01945 WP_252021437.1 377843..378952(+) (pilU) [Vibrio sp. SCSIO 43136]
ATGGAGCTGAATCAGATACTACAGCAGATGGTGGCCAAAAAAGCCTCGGATATTTATATCACGGTTGGCGCCCCTTGTTT
GCTTAGGGTTCATGGCGAGCTGGAAGCTGTAGGTGAGGTGCTCAATGAACACCAAGTGCATACGCTGCTCGACAGTGCGA
TGGATGATGTTAGACGAGGTGAGTTTCGTCGTGACAAAGAAGCAAACTTTGCCATTGTGCGAGATAGCGGGCGCTATCGT
GTCAGCGCATTCTTTCAAAGAGAGCTGCCTGGTGCGGTCATTCGTCGTATCGAAACTCAGATCCCTACGTTTGACGAGCT
CAAGCTACCCGGTGTGCTGCAAGATTTGTCGGTGGCAAAGCGAGGGTTAGTGCTGGTGGTCGGTGCGACGGGCTCCGGTA
AGTCAACAACACTCGCGGCCATGACAGGTCATCGAAATTCCACTCGAACCGGACACATTCTAACAGTGGAAGATCCGATT
GAGTTCGTACACGAGCATAAAAAATGCATCGTGACTCAAAGGGAAGTTGGTTTAGATACCGAAAGTTATGAAGTGGCACT
AAAGAACTCGTTGCGACAGGCTCCCGACGTAATTTTGATTGGGGAAATCCGCTCTCGTGAAACCATGCAGTACGCAATGA
CCTTTGCTGAAACGGGTCACCTGTGTATGGCAACATTGCATGCTAACAATGCCAACCAAGCCATCGAGCGTATTTTACAT
TTAGTGCCAAAAGAACAAAAAGAGCAGTTTCTTTTTGATCTTTCGATGAACTTGAAAGGAGTGATTGCCCAGCAATTGAT
CCGAGATAAGAGTGGTCAGGGACGTCATGGGGTGTTTGAGATATTGCTCAATACACCAAGGGTGGCCGATCTTATTCGTC
GTGGTGATCTGCATGAGCTGAAAACTACTATGGCTAAATCGAGTCAATCTGGGATGCAGACGTTTGACCAATCGTTATAC
GAGTTGATAAGCGCAGGCAAGATCACTGAACAAGATGGCCTGCATAGCGCTGACTCGGCTAACGACCTGCGTATGATGCT
GAAGTCGAAACAGTCGGGAACAAGCAGTAGTTCTGGATTGCTTGATGGGGTTCAGATTGATATGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

78.049

100

0.78
  pilU Pseudomonas stutzeri DSM 10701

56.131

99.458

0.558
  pilU Acinetobacter baylyi ADP1

53.276

95.122

0.507
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.778

97.561

0.417
  pilT Pseudomonas aeruginosa PAK

41.888

91.87

0.385
  pilT Legionella pneumophila strain ERS1305867

41.593

91.87

0.382
  pilT Legionella pneumophila strain Lp02

41.593

91.87

0.382
  pilT Acinetobacter baylyi ADP1

42.424

89.431

0.379
  pilT Acinetobacter nosocomialis M2

41.742

90.244

0.377
  pilT Acinetobacter baumannii strain A118

41.441

90.244

0.374
  pilT Acinetobacter baumannii D1279779

41.441

90.244

0.374
  pilT Vibrio cholerae strain A1552

41.018

90.515

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.515

0.371
  pilT Pseudomonas stutzeri DSM 10701

41.088

89.702

0.369
  pilT Neisseria gonorrhoeae MS11

39.94

90.244

0.36
  pilT Neisseria meningitidis 8013

39.94

90.244

0.36