Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J4N39_RS01940 Genome accession   NZ_CP071848
Coordinates   376786..377832 (+) Length   348 a.a.
NCBI ID   WP_252021435.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43136     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 371786..382832
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N39_RS01900 (J4N39_01900) - 372413..373015 (-) 603 WP_252021419.1 XTP/dITP diphosphatase -
  J4N39_RS01905 (J4N39_01905) - 373096..373539 (-) 444 WP_252021421.1 DUF4426 domain-containing protein -
  J4N39_RS01910 (J4N39_01910) - 373574..373885 (-) 312 WP_252021423.1 helix-turn-helix domain-containing protein -
  J4N39_RS01915 (J4N39_01915) - 373878..374225 (-) 348 WP_252021425.1 type II toxin-antitoxin system RelE/ParE family toxin -
  J4N39_RS01920 (J4N39_01920) yggU 374332..374622 (-) 291 WP_252021427.1 DUF167 family protein YggU -
  J4N39_RS01925 (J4N39_01925) - 374622..375179 (-) 558 WP_252021429.1 YggT family protein -
  J4N39_RS01930 (J4N39_01930) proC 375214..376032 (-) 819 WP_252021431.1 pyrroline-5-carboxylate reductase -
  J4N39_RS01935 (J4N39_01935) - 376057..376761 (-) 705 WP_252021433.1 YggS family pyridoxal phosphate-dependent enzyme -
  J4N39_RS01940 (J4N39_01940) pilT 376786..377832 (+) 1047 WP_252021435.1 type IV pilus twitching motility protein PilT Machinery gene
  J4N39_RS01945 (J4N39_01945) pilU 377843..378952 (+) 1110 WP_252021437.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J4N39_RS01950 (J4N39_01950) ruvX 379049..379474 (-) 426 WP_252021438.1 Holliday junction resolvase RuvX -
  J4N39_RS01955 (J4N39_01955) - 379692..380255 (-) 564 WP_252021439.1 YqgE/AlgH family protein -
  J4N39_RS01960 (J4N39_01960) gshB 380280..381227 (-) 948 WP_252021440.1 glutathione synthase -
  J4N39_RS01965 (J4N39_01965) rsmE 381240..381971 (-) 732 WP_252021441.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  J4N39_RS01970 (J4N39_01970) - 382113..382823 (-) 711 WP_252021442.1 endonuclease -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38405.74 Da        Isoelectric Point: 6.2694

>NTDB_id=548277 J4N39_RS01940 WP_252021435.1 376786..377832(+) (pilT) [Vibrio sp. SCSIO 43136]
MDIAELLDFSVKHNASDLHLSAGVPPMVRVDGDVRKLGVPPLEHGDVHRLVFDIMNDAQRAEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPTEVPTLEQISAPDIFTQISNYEKGLVLVTGPTGSGKSTTLAAMVDHVNRNHNKHILTIED
PIEFVHQTNKSLINQREVHRDTHSFNAALRSALREDPDVILVGELRDQETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGNEKSMVRSMLSESLRAVISQKLLKRIGGGRVACHEIMIANPAIRNLVREDKVAQMISVIQTGAAVGMQTTEQHV
RQLISQGLVDPQEVANKLELDQPSPSGF

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=548277 J4N39_RS01940 WP_252021435.1 376786..377832(+) (pilT) [Vibrio sp. SCSIO 43136]
ATGGATATCGCAGAGTTATTGGATTTTAGTGTAAAGCATAATGCCTCAGATCTACATCTTTCTGCAGGTGTACCGCCGAT
GGTGCGTGTCGATGGTGATGTGCGCAAGTTGGGTGTTCCCCCTTTAGAGCACGGTGATGTCCATCGTTTAGTGTTCGATA
TTATGAATGATGCTCAGCGAGCAGAGTTTGAAGAAAAGCTAGAAGTCGACTTCTCATTTGAGTTACCTAATGTGGGTCGT
TTTCGTGTTAATGCCTTTAATCAATCTCGCGGCTGCTCAGCGGTGTTTCGTACCATTCCTACCGAAGTACCAACATTAGA
ACAGATCTCAGCCCCTGATATTTTTACCCAGATCTCCAATTATGAAAAAGGTTTAGTTCTGGTCACAGGTCCAACGGGAT
CGGGTAAGTCAACGACATTAGCTGCCATGGTGGATCACGTTAACCGCAATCACAATAAACACATTTTGACCATTGAAGAC
CCAATTGAGTTTGTACACCAAACTAACAAATCTTTGATCAACCAACGTGAAGTGCACCGAGACACCCATAGCTTTAACGC
GGCACTGCGCTCTGCGCTGCGTGAGGATCCAGATGTCATCTTAGTGGGTGAGCTACGTGACCAAGAGACAATTCGCCTAG
CGTTAACTGCGGCAGAAACGGGTCATTTGGTGTTTGGTACTCTGCACACTAGCAGCGCTGCAAAAACTATCGACAGGATC
ATCGATGTTTTCCCGGGTAATGAGAAGTCGATGGTGCGCTCAATGTTATCTGAATCCTTACGCGCCGTAATTTCGCAAAA
GCTGCTCAAACGTATCGGTGGTGGACGAGTTGCTTGCCATGAAATCATGATTGCTAATCCGGCCATTCGAAACTTGGTGC
GAGAAGATAAGGTAGCGCAGATGATCTCGGTGATTCAAACAGGCGCAGCAGTCGGCATGCAAACGACCGAGCAGCACGTG
CGTCAGCTTATCTCTCAAGGGCTGGTTGACCCACAAGAGGTGGCGAACAAGCTTGAGCTAGACCAACCATCACCAAGTGG
ATTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

84.457

97.989

0.828
  pilT Vibrio cholerae strain A1552

84.457

97.989

0.828
  pilT Acinetobacter nosocomialis M2

73.494

95.402

0.701
  pilT Acinetobacter baumannii strain A118

73.193

95.402

0.698
  pilT Acinetobacter baumannii D1279779

73.193

95.402

0.698
  pilT Pseudomonas aeruginosa PAK

68.824

97.701

0.672
  pilT Acinetobacter baylyi ADP1

70.909

94.828

0.672
  pilT Pseudomonas stutzeri DSM 10701

68.843

96.839

0.667
  pilT Legionella pneumophila strain ERS1305867

69.301

94.54

0.655
  pilT Legionella pneumophila strain Lp02

69.301

94.54

0.655
  pilT Neisseria meningitidis 8013

65.68

97.126

0.638
  pilT Neisseria gonorrhoeae MS11

65.465

95.69

0.626
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.312

99.425

0.52
  pilU Vibrio cholerae strain A1552

39.548

100

0.402
  pilU Pseudomonas stutzeri DSM 10701

40.854

94.253

0.385