Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J1M35_RS19335 Genome accession   NZ_CP071796
Coordinates   4073151..4074194 (+) Length   347 a.a.
NCBI ID   WP_208008899.1    Uniprot ID   A0A975CG33
Organism   Ottowia testudinis strain 27C     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4068151..4079194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1M35_RS19310 (J1M35_19310) - 4068376..4068657 (+) 282 WP_208008894.1 DUF3567 domain-containing protein -
  J1M35_RS19315 (J1M35_19315) - 4068962..4069429 (-) 468 WP_208008895.1 YXWGXW repeat-containing protein -
  J1M35_RS19320 (J1M35_19320) - 4069669..4071270 (-) 1602 WP_208008896.1 AMP-binding protein -
  J1M35_RS19325 (J1M35_19325) purU 4071362..4072210 (+) 849 WP_208008897.1 formyltetrahydrofolate deformylase -
  J1M35_RS19330 (J1M35_19330) - 4072416..4073111 (-) 696 WP_208008898.1 YggS family pyridoxal phosphate-dependent enzyme -
  J1M35_RS19335 (J1M35_19335) pilT 4073151..4074194 (+) 1044 WP_208008899.1 type IV pilus twitching motility protein PilT Machinery gene
  J1M35_RS19340 (J1M35_19340) pilU 4074436..4075572 (+) 1137 WP_208008900.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J1M35_RS19345 (J1M35_19345) - 4075785..4076699 (+) 915 WP_208008901.1 NAD(P)-dependent oxidoreductase -
  J1M35_RS19350 (J1M35_19350) - 4076834..4077604 (-) 771 WP_208008902.1 BON domain-containing protein -
  J1M35_RS19355 (J1M35_19355) - 4077604..4078200 (-) 597 WP_208008903.1 SIS domain-containing protein -
  J1M35_RS19360 (J1M35_19360) - 4078375..4078761 (-) 387 WP_208008904.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38282.08 Da        Isoelectric Point: 6.8357

>NTDB_id=547815 J1M35_RS19335 WP_208008899.1 4073151..4074194(+) (pilT) [Ottowia testudinis strain 27C]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRLNVDPLEHKQVFAMLYDIMNDTQRKVYEENLEVDFSFEIEGLAR
FRVNAFNQNRGAAGVLRTIPSKILTLEQLNAPKIFGDLALKPRGMVLVTGPTGSGKSTTLAAMVNHLNETEYGHVLTVED
PIEFVHESKKCLINQREVGPMTHSFANALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMIRAMLSESLQAVISQTLCKTKDGNGRVAAHEIMLGTPAIRNLIREAKVAQMYSSIQTGNSMGMQTLDQN
LTDLVRRNVISPAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=547815 J1M35_RS19335 WP_208008899.1 4073151..4074194(+) (pilT) [Ottowia testudinis strain 27C]
GTGGACATTACCCAGCTGCTGGCTTTCAGCGTCAAGAACAAGGCCTCCGACCTGCACCTGTCGGCCGGCCTGCCGCCCAT
GATCCGTGTGCACGGCGACGTGCGCCGCCTGAACGTGGACCCGCTGGAACACAAGCAGGTGTTCGCGATGCTCTACGACA
TCATGAACGACACCCAGCGCAAGGTGTACGAGGAAAACCTGGAAGTCGACTTCTCGTTCGAAATCGAAGGCCTGGCGCGC
TTTCGCGTCAACGCCTTCAACCAGAACCGGGGCGCCGCCGGCGTGCTGCGGACGATTCCCAGCAAGATCCTGACGCTGGA
GCAGCTCAACGCGCCCAAGATCTTCGGCGACCTGGCGCTCAAGCCGCGCGGCATGGTGCTGGTGACCGGCCCCACCGGCT
CGGGCAAATCGACCACGCTGGCCGCCATGGTCAACCACCTGAACGAAACCGAATACGGCCACGTGCTCACCGTGGAAGAC
CCGATCGAATTCGTGCACGAGTCCAAGAAGTGCCTGATCAACCAGCGCGAAGTGGGGCCGATGACGCACAGCTTTGCCAA
CGCGCTGCGCAGCGCGCTGCGCGAAGACCCGGACGCGATCCTGGTCGGTGAGATGCGCGATCTGGAAACGATTCGCCTGG
CCATGACGGCCGCCGAAACCGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAAACCATCGACCGCATC
ATCGACGTCTTCCCGGCGGAAGAAAAGGAAATGATCCGCGCCATGCTGTCCGAGTCGCTGCAGGCTGTGATTTCACAAAC
CCTGTGCAAGACCAAGGACGGCAACGGACGTGTGGCGGCGCACGAGATCATGCTGGGCACGCCCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCGTCCATCCAAACCGGCAACTCGATGGGCATGCAGACGCTGGACCAGAAC
CTGACCGACCTGGTGCGCCGCAACGTGATCAGCCCGGCCGAGGCGCGCGGGAAAGCCAAGATCCCCGAGAACTTTCCCGG
GTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.942

99.424

0.755
  pilT Acinetobacter baumannii D1279779

75.942

99.424

0.755
  pilT Acinetobacter baumannii strain A118

75.942

99.424

0.755
  pilT Pseudomonas stutzeri DSM 10701

74.493

99.424

0.741
  pilT Acinetobacter baylyi ADP1

73.333

99.424

0.729
  pilT Legionella pneumophila strain Lp02

72.271

97.695

0.706
  pilT Legionella pneumophila strain ERS1305867

72.271

97.695

0.706
  pilT Neisseria meningitidis 8013

68.116

99.424

0.677
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.155

98.271

0.66
  pilT Vibrio cholerae strain A1552

67.155

98.271

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

99.424

0.513
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Vibrio cholerae strain A1552

42.09

96.542

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398