Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   J1M35_RS19340 Genome accession   NZ_CP071796
Coordinates   4074436..4075572 (+) Length   378 a.a.
NCBI ID   WP_208008900.1    Uniprot ID   -
Organism   Ottowia testudinis strain 27C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4069436..4080572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1M35_RS19320 (J1M35_19320) - 4069669..4071270 (-) 1602 WP_208008896.1 AMP-binding protein -
  J1M35_RS19325 (J1M35_19325) purU 4071362..4072210 (+) 849 WP_208008897.1 formyltetrahydrofolate deformylase -
  J1M35_RS19330 (J1M35_19330) - 4072416..4073111 (-) 696 WP_208008898.1 YggS family pyridoxal phosphate-dependent enzyme -
  J1M35_RS19335 (J1M35_19335) pilT 4073151..4074194 (+) 1044 WP_208008899.1 type IV pilus twitching motility protein PilT Machinery gene
  J1M35_RS19340 (J1M35_19340) pilU 4074436..4075572 (+) 1137 WP_208008900.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J1M35_RS19345 (J1M35_19345) - 4075785..4076699 (+) 915 WP_208008901.1 NAD(P)-dependent oxidoreductase -
  J1M35_RS19350 (J1M35_19350) - 4076834..4077604 (-) 771 WP_208008902.1 BON domain-containing protein -
  J1M35_RS19355 (J1M35_19355) - 4077604..4078200 (-) 597 WP_208008903.1 SIS domain-containing protein -
  J1M35_RS19360 (J1M35_19360) - 4078375..4078761 (-) 387 WP_208008904.1 YraN family protein -
  J1M35_RS19365 (J1M35_19365) rsmI 4078795..4079718 (+) 924 WP_208008905.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41954.32 Da        Isoelectric Point: 6.7691

>NTDB_id=547816 J1M35_RS19340 WP_208008900.1 4074436..4075572(+) (pilU) [Ottowia testudinis strain 27C]
MERDQASKFINDLLKLMLTRKGSDLFITADFPPAIKVDGKVTKVSSQNLTAGHTLALVRSVMNDKQAAEFERTKECNFAI
SPAGIGRFRCNAFVQQGHVGMVMRVIPQELPTIDSLGMPQILKDVSMSKRGLTILVGATGSGKSTTLAAMVDWRNVNSQG
HIITIEDPVEFVHPHKNCVVTQREVGLDTEDWDAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNLRSLISQRLIPKQDGKGRTAVVEILLNSPLISDLIFKGEVGEIKEIMKKSRELG
MQTFDQALFDAFEANLINYEDALRNADSANDLRLQIKLTSKRAKSLDLAAGTEHLQIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=547816 J1M35_RS19340 WP_208008900.1 4074436..4075572(+) (pilU) [Ottowia testudinis strain 27C]
ATGGAACGCGATCAGGCCAGTAAATTCATTAATGATCTGCTCAAGCTCATGCTCACGCGCAAGGGCAGCGATTTGTTCAT
CACGGCCGACTTTCCGCCAGCCATCAAGGTCGACGGCAAGGTAACCAAGGTGTCGTCGCAAAACCTCACCGCCGGCCACA
CGCTGGCGCTGGTGCGTTCGGTGATGAACGACAAGCAGGCGGCGGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCCATT
TCGCCCGCTGGCATCGGGCGCTTCCGCTGCAACGCCTTCGTGCAGCAAGGCCACGTGGGCATGGTGATGCGGGTGATTCC
GCAAGAGTTGCCCACCATCGACTCGCTCGGCATGCCGCAGATCCTGAAGGACGTGTCGATGTCCAAGCGCGGCCTGACCA
TCCTGGTGGGCGCCACCGGCTCGGGCAAGTCGACCACGCTGGCCGCCATGGTCGACTGGCGCAACGTCAACAGCCAGGGC
CACATCATCACGATCGAGGACCCGGTCGAATTCGTGCACCCTCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGTCT
GGACACCGAGGATTGGGACGCGGCGCTGAAAAACACGCTGCGCCAGGCGCCCGACGTCATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAGCACGCCGTGGCCTTTGCCGAAACCGGCCACCTCTGCCTGGCCACGCTGCACGCCAACTCGGCC
AACCAGGCGCTCGACCGGATCATCAACTTCTTCCCGGAAGAGCGGCGCCAGCAGCTGCTGATGGATTTGTCGCTCAACCT
GCGTTCGCTGATCTCGCAGCGCCTGATCCCCAAGCAGGACGGCAAGGGGCGCACGGCGGTGGTCGAGATCCTGCTCAATT
CGCCACTGATCAGCGACCTGATATTCAAGGGCGAGGTCGGCGAGATCAAGGAGATCATGAAGAAGAGCCGAGAGCTGGGC
ATGCAGACCTTCGATCAGGCCCTGTTCGACGCCTTCGAGGCCAACTTGATCAACTACGAGGACGCGCTGCGCAACGCCGA
TTCGGCCAACGACCTGCGCCTGCAGATCAAGCTGACCAGCAAGCGCGCCAAGTCGCTCGACCTGGCGGCTGGCACCGAGC
ACTTGCAAATTGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(99-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.891

92.328

0.571
  pilU Acinetobacter baylyi ADP1

57.66

94.974

0.548
  pilU Vibrio cholerae strain A1552

53.719

96.032

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

89.153

0.399
  pilT Pseudomonas aeruginosa PAK

43.917

89.153

0.392
  pilT Pseudomonas stutzeri DSM 10701

41.543

89.153

0.37
  pilT Legionella pneumophila strain ERS1305867

41.441

88.095

0.365
  pilT Legionella pneumophila strain Lp02

41.441

88.095

0.365
  pilT Acinetobacter baumannii D1279779

42.947

84.392

0.362
  pilT Acinetobacter baumannii strain A118

42.947

84.392

0.362
  pilT Acinetobacter nosocomialis M2

42.947

84.392

0.362
  pilT Acinetobacter baylyi ADP1

41.141

88.095

0.362