Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J0X00_RS13905 Genome accession   NZ_CP071708
Coordinates   1652786..1653823 (+) Length   345 a.a.
NCBI ID   WP_136487563.1    Uniprot ID   -
Organism   Vibrio sp. ABG19     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1647786..1658823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0X00_RS13875 (J0X00_13870) - 1648824..1649447 (-) 624 WP_282478272.1 XTP/dITP diphosphatase -
  J0X00_RS13880 (J0X00_13875) - 1649465..1649866 (-) 402 WP_256612999.1 DUF4426 domain-containing protein -
  J0X00_RS13885 (J0X00_13880) yggU 1650035..1650325 (-) 291 WP_136487567.1 DUF167 family protein YggU -
  J0X00_RS13890 (J0X00_13885) - 1650325..1650882 (-) 558 WP_136487566.1 YggT family protein -
  J0X00_RS13895 (J0X00_13890) proC 1651005..1651823 (-) 819 WP_282477022.1 pyrroline-5-carboxylate reductase -
  J0X00_RS13900 (J0X00_13895) - 1652035..1652745 (-) 711 WP_282477023.1 YggS family pyridoxal phosphate-dependent enzyme -
  J0X00_RS13905 (J0X00_13900) pilT 1652786..1653823 (+) 1038 WP_136487563.1 type IV pilus twitching motility protein PilT Machinery gene
  J0X00_RS13910 (J0X00_13905) pilU 1653869..1654984 (+) 1116 WP_136487630.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J0X00_RS13915 (J0X00_13910) ruvX 1655142..1655567 (-) 426 WP_136487562.1 Holliday junction resolvase RuvX -
  J0X00_RS13920 (J0X00_13915) - 1655596..1656159 (-) 564 WP_136487561.1 YqgE/AlgH family protein -
  J0X00_RS13925 (J0X00_13920) gshB 1656237..1657193 (-) 957 WP_136487560.1 glutathione synthase -
  J0X00_RS13930 (J0X00_13925) rsmE 1657205..1657936 (-) 732 WP_218562396.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38100.65 Da        Isoelectric Point: 6.6687

>NTDB_id=547152 J0X00_RS13905 WP_136487563.1 1652786..1653823(+) (pilT) [Vibrio sp. ABG19]
MDISELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFSHADVHRLIFEIMNDAQRSEYEERLEVDFSFELPSVGR
FRVNAFHQARGASAVLRTIPTTIPSLEQLEAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGNDKSMVRSMLSESLRAVIAQKLLKRVGGGRVACHEVMLATPAIRNLIREDKVAQMYSIIQTGAMHGMQTMEQNA
KQLLAKGLVDQAEVQQKIATDIAGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=547152 J0X00_RS13905 WP_136487563.1 1652786..1653823(+) (pilT) [Vibrio sp. ABG19]
ATGGATATCTCTGAATTACTGGAATTTAGTGTAAAACATAATGCGTCAGATCTACATCTTTCTGCAGGCGTTCCTCCGAT
GGTTCGTATCGACGGCGAGGTGAGAAAGCTTGGTGTGCCTGCTTTTAGCCATGCTGACGTGCATCGATTAATTTTTGAAA
TTATGAACGATGCCCAGCGCAGCGAGTATGAAGAACGGCTCGAAGTGGACTTTTCCTTTGAACTGCCGTCGGTCGGCCGT
TTCCGGGTCAACGCATTTCATCAGGCGCGTGGTGCCTCAGCGGTGCTGCGGACCATCCCGACCACGATTCCAAGTTTAGA
GCAGCTGGAAGCGCCGGAGATCTTCAGTAAAATCGCCAACTATGAGAAAGGCCTGGTGCTGGTCACCGGCCCGACCGGTT
CCGGTAAATCGACCACACTGGCGGCGATGGTGGATTACATCAATGCCCACCATAATAAACATATCCTGACCATTGAAGAC
CCGATCGAGTTTGTCCATAGCAACAATAAATGCCTGATTAACCAGCGTGAAGTGCACCGCGATACCCATAGCTTTAAAAA
TGCGCTGCGCAGCGCGCTGCGTGAGGATCCGGATGTGATTCTGGTTGGTGAGCTGCGTGACCAGGAGACTATCAGCCTGG
CGCTGACCGCCGCAGAAACCGGCCACTTAGTGTTTGGTACCCTGCACACCAGCTCGGCGGCGAAAACCATCGACCGGATT
ATCGATGTCTTCCCCGGCAATGATAAATCTATGGTGCGCTCCATGCTGTCGGAGTCACTGCGCGCGGTGATCGCCCAGAA
GCTGCTCAAACGGGTCGGGGGCGGCCGGGTGGCCTGTCATGAGGTGATGCTGGCCACTCCGGCGATCCGTAACTTGATCC
GCGAGGATAAAGTGGCGCAGATGTACTCGATTATCCAGACCGGTGCCATGCACGGCATGCAGACCATGGAGCAGAATGCC
AAGCAGCTGCTGGCGAAAGGGCTGGTGGATCAGGCTGAAGTGCAGCAAAAAATCGCCACCGATATTGCTGGGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

92.174

100

0.922
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

92.174

100

0.922
  pilT Acinetobacter baylyi ADP1

71.818

95.652

0.687
  pilT Acinetobacter baumannii D1279779

71.429

95.362

0.681
  pilT Acinetobacter baumannii strain A118

71.429

95.362

0.681
  pilT Pseudomonas stutzeri DSM 10701

69.733

97.681

0.681
  pilT Acinetobacter nosocomialis M2

71.125

95.362

0.678
  pilT Pseudomonas aeruginosa PAK

67.656

97.681

0.661
  pilT Legionella pneumophila strain Lp02

67.372

95.942

0.646
  pilT Legionella pneumophila strain ERS1305867

67.372

95.942

0.646
  pilT Neisseria meningitidis 8013

64.545

95.652

0.617
  pilT Neisseria gonorrhoeae MS11

64.242

95.652

0.614
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.515

95.652

0.493
  pilU Vibrio cholerae strain A1552

44

94.203

0.414
  pilU Pseudomonas stutzeri DSM 10701

41.768

95.072

0.397
  pilU Acinetobacter baylyi ADP1

41.25

92.754

0.383