Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   J0X00_RS13910 Genome accession   NZ_CP071708
Coordinates   1653869..1654984 (+) Length   371 a.a.
NCBI ID   WP_136487630.1    Uniprot ID   -
Organism   Vibrio sp. ABG19     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1648869..1659984
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0X00_RS13880 (J0X00_13875) - 1649465..1649866 (-) 402 WP_256612999.1 DUF4426 domain-containing protein -
  J0X00_RS13885 (J0X00_13880) yggU 1650035..1650325 (-) 291 WP_136487567.1 DUF167 family protein YggU -
  J0X00_RS13890 (J0X00_13885) - 1650325..1650882 (-) 558 WP_136487566.1 YggT family protein -
  J0X00_RS13895 (J0X00_13890) proC 1651005..1651823 (-) 819 WP_282477022.1 pyrroline-5-carboxylate reductase -
  J0X00_RS13900 (J0X00_13895) - 1652035..1652745 (-) 711 WP_282477023.1 YggS family pyridoxal phosphate-dependent enzyme -
  J0X00_RS13905 (J0X00_13900) pilT 1652786..1653823 (+) 1038 WP_136487563.1 type IV pilus twitching motility protein PilT Machinery gene
  J0X00_RS13910 (J0X00_13905) pilU 1653869..1654984 (+) 1116 WP_136487630.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J0X00_RS13915 (J0X00_13910) ruvX 1655142..1655567 (-) 426 WP_136487562.1 Holliday junction resolvase RuvX -
  J0X00_RS13920 (J0X00_13915) - 1655596..1656159 (-) 564 WP_136487561.1 YqgE/AlgH family protein -
  J0X00_RS13925 (J0X00_13920) gshB 1656237..1657193 (-) 957 WP_136487560.1 glutathione synthase -
  J0X00_RS13930 (J0X00_13925) rsmE 1657205..1657936 (-) 732 WP_218562396.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  J0X00_RS13935 (J0X00_13930) - 1658160..1658876 (-) 717 WP_136487558.1 endonuclease -
  J0X00_RS13940 (J0X00_13935) - 1659058..1659564 (-) 507 WP_206208448.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40839.42 Da        Isoelectric Point: 5.2856

>NTDB_id=547153 J0X00_RS13910 WP_136487630.1 1653869..1654984(+) (pilU) [Vibrio sp. ABG19]
MQLDECLQGMVTRKASDIYITVGAPLLYRVNGALEAEGSALGSAQVGQLLDAMMDSARQQEFAASHEANFALVRDFGRFR
VSAFMQREQPGAVIRRIETDIPTFEQLDLPPVLKDLALAKRGLVLVVGATGSGKSTTLAAMTGYRNAERSGHILSVEDPI
EFVHPHQRSIVTQREVGLDTDSFEVALKNSLRQAPDMIVIGEIRTRETMEYAMTFAETGHLCLATLHANNANQALERILH
LVSKERREQFLFDLSLNLQGIIAQQLLADKQGQGRHGVFEILLNSPRVADLIRRGDLHELKATMAKSRENGMQTFDQALY
QLVAEDKIDEQEALHSADSANDLRLMLKTRSGTEGSALSGGSLANVQIDMD

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=547153 J0X00_RS13910 WP_136487630.1 1653869..1654984(+) (pilU) [Vibrio sp. ABG19]
ATGCAACTCGATGAGTGCCTGCAGGGCATGGTGACGCGTAAAGCGTCCGATATCTATATCACGGTCGGCGCGCCGCTGCT
GTACCGGGTCAACGGCGCACTGGAGGCTGAGGGCAGTGCACTTGGCTCGGCTCAGGTCGGGCAACTGCTGGATGCGATGA
TGGACAGTGCCCGCCAGCAAGAGTTTGCCGCCAGCCATGAAGCTAATTTTGCCCTGGTGCGTGACTTTGGCCGTTTCCGG
GTCAGTGCTTTTATGCAGCGCGAGCAGCCGGGCGCGGTGATCCGCCGCATCGAAACCGACATCCCGACCTTTGAGCAGCT
CGATTTGCCGCCGGTACTGAAAGATCTGGCACTGGCCAAGCGCGGCCTGGTGCTGGTGGTGGGAGCGACCGGCTCCGGTA
AGTCGACCACCCTGGCGGCGATGACCGGCTATCGCAATGCCGAGCGCAGCGGGCATATCCTCAGCGTGGAAGATCCGATT
GAGTTCGTTCATCCCCATCAGCGCAGTATCGTGACCCAGCGTGAAGTCGGCCTGGATACCGACAGTTTTGAAGTGGCGCT
GAAAAACTCGCTGCGTCAGGCACCGGACATGATCGTCATCGGGGAGATCCGCACACGTGAGACGATGGAATACGCCATGA
CCTTTGCCGAAACCGGCCATCTGTGTCTGGCTACCCTGCACGCTAATAATGCCAACCAGGCGCTGGAGCGCATTTTACAT
CTGGTCAGCAAAGAGCGCCGCGAGCAGTTTCTGTTTGATCTGTCGCTCAACCTGCAAGGGATTATCGCGCAGCAATTGCT
GGCGGATAAACAGGGTCAGGGGCGGCACGGGGTGTTTGAAATATTGCTCAACAGCCCGCGCGTGGCGGATCTTATCCGGC
GCGGCGATCTGCATGAACTGAAAGCGACCATGGCCAAATCACGTGAGAACGGCATGCAGACCTTTGATCAGGCGCTGTAT
CAGCTGGTGGCAGAGGATAAAATCGATGAGCAGGAAGCGCTGCACAGTGCCGATTCGGCCAATGATCTGCGTCTGATGCT
CAAAACCCGCTCGGGCACAGAGGGTTCTGCGCTGTCCGGCGGTTCACTGGCGAATGTGCAGATTGATATGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

78.167

100

0.782
  pilU Pseudomonas stutzeri DSM 10701

57.967

98.113

0.569
  pilU Acinetobacter baylyi ADP1

52.422

94.609

0.496
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.884

97.574

0.399
  pilT Legionella pneumophila strain Lp02

40.708

91.375

0.372
  pilT Legionella pneumophila strain ERS1305867

40.708

91.375

0.372
  pilT Vibrio cholerae strain A1552

41.018

90.027

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.027

0.369
  pilT Acinetobacter baumannii D1279779

39.823

91.375

0.364
  pilT Acinetobacter baumannii strain A118

39.823

91.375

0.364
  pilT Acinetobacter nosocomialis M2

39.528

91.375

0.361