Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JYB85_RS04975 Genome accession   NZ_CP071502
Coordinates   1169352..1170389 (+) Length   345 a.a.
NCBI ID   WP_207322299.1    Uniprot ID   -
Organism   Shewanella sedimentimangrovi strain FJAT-52962     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1164352..1175389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JYB85_RS04940 (JYB85_04940) hemW 1164425..1165561 (-) 1137 WP_207381296.1 radical SAM family heme chaperone HemW -
  JYB85_RS04945 (JYB85_04945) rdgB 1165565..1166164 (-) 600 WP_207381297.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  JYB85_RS04950 (JYB85_04950) - 1166263..1166697 (-) 435 WP_207381298.1 DUF4426 domain-containing protein -
  JYB85_RS04955 (JYB85_04955) yggU 1166775..1167062 (-) 288 WP_207381299.1 DUF167 family protein YggU -
  JYB85_RS04960 (JYB85_04960) - 1167062..1167610 (-) 549 WP_207381300.1 YggT family protein -
  JYB85_RS04965 (JYB85_04965) proC 1167651..1168466 (-) 816 WP_207381301.1 pyrroline-5-carboxylate reductase -
  JYB85_RS04970 (JYB85_04970) - 1168609..1169316 (-) 708 WP_207381302.1 YggS family pyridoxal phosphate-dependent enzyme -
  JYB85_RS04975 (JYB85_04975) pilT 1169352..1170389 (+) 1038 WP_207322299.1 type IV pilus twitching motility protein PilT Machinery gene
  JYB85_RS04980 (JYB85_04980) pilU 1170412..1171524 (+) 1113 WP_207381303.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JYB85_RS04985 (JYB85_04985) - 1171605..1172174 (+) 570 WP_207381304.1 glutathione peroxidase -
  JYB85_RS04990 (JYB85_04990) hemH 1172180..1173184 (+) 1005 WP_228289692.1 ferrochelatase -
  JYB85_RS04995 (JYB85_04995) ruvX 1173277..1173699 (-) 423 WP_207381305.1 Holliday junction resolvase RuvX -
  JYB85_RS05000 (JYB85_05000) - 1173707..1174270 (-) 564 WP_207325661.1 YqgE/AlgH family protein -
  JYB85_RS05005 (JYB85_05005) yciH 1174287..1174616 (-) 330 WP_207322304.1 stress response translation initiation inhibitor YciH -
  JYB85_RS05010 (JYB85_05010) - 1174778..1175119 (-) 342 WP_207381306.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38335.01 Da        Isoelectric Point: 6.9096

>NTDB_id=545713 JYB85_RS04975 WP_207322299.1 1169352..1170389(+) (pilT) [Shewanella sedimentimangrovi strain FJAT-52962]
MEITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALDHQGVHNLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSEILSLETLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAGMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=545713 JYB85_RS04975 WP_207322299.1 1169352..1170389(+) (pilT) [Shewanella sedimentimangrovi strain FJAT-52962]
ATGGAAATCACTGAGTTACTTGCCTTTAGTGTAAAACACAAGGCCTCGGATCTGCACCTCTCAGCCGGTGTGCCTCCCAT
GATCCGTGTCGATGGCGAAGTTCGAAAAATCAACCTGCCGGCGCTGGATCACCAAGGCGTACACAATCTTGTGTACGACA
TTATGAATGACAAGCAGCGCAAAGACTATGAAGAGCACCTGGAAATCGACTTTTCCTTCGAGGTGCCCAATCTGGCACGC
TTCCGTGTTAACGCCTTCAACCAATCCCGCGGTGCCGCCGCCGTGTTCCGTACCATTCCCAGCGAAATCCTGTCACTGGA
AACCCTGGGCGCGCCGGAAATTTTCAAGAAAATTGCCAGTTTCCCCCGCGGTCTGGTGCTGGTAACCGGCCCCACGGGTT
CAGGTAAAAGTACCACCCTGGCGGGCATGATAGATTATGTGAACGAGAACCGGCACGATCACATTCTCACCATCGAAGAC
CCCATCGAATTCGTGCACCAGAACAAGCAGTGCCTTATCAACCAGCGGGAAGTGCACAGGCACACCCACAGCTTCAACGC
GGCGCTGCGCAGCGCACTGCGTGAAGACCCGGACGTGATCCTGGTGGGTGAGATGCGTGACCTTGAAACCATACGTCTGG
CGATGACGGCGGCGGAAACCGGTCACCTGGTGTTCGGTACCCTGCACACCACCTCGGCGGCGAAGACCATTGACCGTGTG
GTGGACGTGTTCCCCGCCGGCGAAAAAGACATGGTGCGTACCATGTTGTCCGAATCCCTGCAGGCGGTTATTTCCCAGAC
CCTGATTAAGAAAATCGGTGGCGGTCGGGTGGCGGCCCATGAGATCATGATGGGAACACCGGCCATTCGTAACCTTATCC
GGGAAGACAAGGTGGCGCAGATGTATTCTGCCATTCAAACGGGTATGGCCCACGGTATGCAAACCCTGGAGCAATGTCTG
CAGAATCTGGTAAACCGTGGCCTTATCACCCGTGAAGACGCCATGGCCAAGAGCTCCAACAAACAGGCTAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.409

99.13

0.797
  pilT Acinetobacter baumannii D1279779

80.409

99.13

0.797
  pilT Acinetobacter baumannii strain A118

80.409

99.13

0.797
  pilT Acinetobacter baylyi ADP1

77.843

99.42

0.774
  pilT Pseudomonas aeruginosa PAK

78.994

97.971

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.101

100

0.771
  pilT Legionella pneumophila strain Lp02

75.66

98.841

0.748
  pilT Legionella pneumophila strain ERS1305867

75.66

98.841

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.7

97.681

0.71
  pilT Vibrio cholerae strain A1552

72.7

97.681

0.71
  pilT Neisseria meningitidis 8013

69.477

99.71

0.693
  pilT Neisseria gonorrhoeae MS11

69.186

99.71

0.69
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.261

0.493
  pilU Pseudomonas stutzeri DSM 10701

42.566

99.42

0.423
  pilU Vibrio cholerae strain A1552

41.399

99.42

0.412
  pilU Acinetobacter baylyi ADP1

40.356

97.681

0.394