Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JYB85_RS04980 Genome accession   NZ_CP071502
Coordinates   1170412..1171524 (+) Length   370 a.a.
NCBI ID   WP_207381303.1    Uniprot ID   -
Organism   Shewanella sedimentimangrovi strain FJAT-52962     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1165412..1176524
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JYB85_RS04945 (JYB85_04945) rdgB 1165565..1166164 (-) 600 WP_207381297.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  JYB85_RS04950 (JYB85_04950) - 1166263..1166697 (-) 435 WP_207381298.1 DUF4426 domain-containing protein -
  JYB85_RS04955 (JYB85_04955) yggU 1166775..1167062 (-) 288 WP_207381299.1 DUF167 family protein YggU -
  JYB85_RS04960 (JYB85_04960) - 1167062..1167610 (-) 549 WP_207381300.1 YggT family protein -
  JYB85_RS04965 (JYB85_04965) proC 1167651..1168466 (-) 816 WP_207381301.1 pyrroline-5-carboxylate reductase -
  JYB85_RS04970 (JYB85_04970) - 1168609..1169316 (-) 708 WP_207381302.1 YggS family pyridoxal phosphate-dependent enzyme -
  JYB85_RS04975 (JYB85_04975) pilT 1169352..1170389 (+) 1038 WP_207322299.1 type IV pilus twitching motility protein PilT Machinery gene
  JYB85_RS04980 (JYB85_04980) pilU 1170412..1171524 (+) 1113 WP_207381303.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JYB85_RS04985 (JYB85_04985) - 1171605..1172174 (+) 570 WP_207381304.1 glutathione peroxidase -
  JYB85_RS04990 (JYB85_04990) hemH 1172180..1173184 (+) 1005 WP_228289692.1 ferrochelatase -
  JYB85_RS04995 (JYB85_04995) ruvX 1173277..1173699 (-) 423 WP_207381305.1 Holliday junction resolvase RuvX -
  JYB85_RS05000 (JYB85_05000) - 1173707..1174270 (-) 564 WP_207325661.1 YqgE/AlgH family protein -
  JYB85_RS05005 (JYB85_05005) yciH 1174287..1174616 (-) 330 WP_207322304.1 stress response translation initiation inhibitor YciH -
  JYB85_RS05010 (JYB85_05010) - 1174778..1175119 (-) 342 WP_207381306.1 hypothetical protein -
  JYB85_RS05015 (JYB85_05015) - 1175338..1175895 (+) 558 WP_207323104.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41068.02 Da        Isoelectric Point: 6.1894

>NTDB_id=545714 JYB85_RS04980 WP_207381303.1 1170412..1171524(+) (pilU) [Shewanella sedimentimangrovi strain FJAT-52962]
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAENAFNPAQALDFVESLMSDAQKKEFHETRECNFAFAAKELGR
FRVSAFWQRESPGCVMRRIETKIPEMDDLKLPAILKDLVMSKRGLIIMVGGTGTGKSTSLAAMVGYRNGHARGHILTIED
PVEFVHDHRQSIITQREVGIDTDSFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHHQLLFDLSLNLRGIVAQQLVPKADGTGRRAAIEILINTPRVSSIIQKNELHLLKDTMSKSNEQGMQTFDQA
LFNLYQEGEISYADALHHADSPNDLRLMIKLASKDSGGSGFLEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=545714 JYB85_RS04980 WP_207381303.1 1170412..1171524(+) (pilU) [Shewanella sedimentimangrovi strain FJAT-52962]
ATGGATGTACGTCCGTTTCTGAAGGTGATGGTCGAGCGCAAGGCCTCGGATCTGTTCATCACCGCAGGTTTCCCGCCCAG
CGCCAAGATAGACGGTGAGCTGCGGCCGCTGGCGGAAAATGCCTTCAACCCGGCCCAGGCGCTGGACTTTGTCGAGTCGC
TGATGAGCGACGCCCAGAAAAAAGAATTCCACGAAACCCGTGAATGTAACTTCGCCTTTGCGGCCAAGGAGCTGGGACGT
TTCCGTGTCAGCGCCTTCTGGCAGCGGGAATCTCCAGGTTGCGTGATGCGCCGCATCGAAACCAAGATCCCCGAGATGGA
CGATCTCAAGTTGCCGGCCATCCTCAAGGATCTGGTGATGAGCAAGCGCGGTCTTATTATCATGGTCGGTGGTACCGGTA
CTGGTAAGTCCACATCGCTGGCCGCCATGGTGGGCTATCGCAACGGTCACGCCCGCGGTCATATTCTCACCATCGAAGAT
CCGGTGGAATTTGTTCACGACCACAGGCAAAGCATCATTACCCAGCGTGAGGTGGGCATAGATACCGACTCCTTCGACGC
GGCGCTGAAAAGCTCCCTGCGCCAGGCGCCGGATGTGATCCTCATCGGTGAGATCCGCAGCCAGGAAACCATGGAGTTCG
CCCTGTCGTTCGCCGAAACCGGTCACCTGTGTATGGCGACCCTGCACGCCAATAACGCCAACCAGGCGTTGGACCGCATC
ATGCACCTGGTGCCTGAAAGCAAACATCATCAATTGCTGTTCGATCTGTCGCTGAACCTGCGCGGCATTGTGGCTCAGCA
GCTGGTACCCAAGGCCGACGGCACCGGCCGCCGGGCAGCCATCGAAATTCTGATCAATACCCCCAGGGTGTCGAGCATTA
TCCAGAAGAACGAGTTGCATCTGCTGAAAGACACCATGTCCAAGTCCAACGAACAGGGCATGCAGACCTTCGACCAGGCG
CTGTTCAACCTGTATCAGGAAGGTGAAATCAGTTACGCCGATGCGCTGCACCATGCAGACTCGCCCAACGATCTGCGTCT
GATGATCAAGCTCGCCAGCAAGGACTCCGGCGGTTCAGGCTTTTTGGAAGGTGTGACCCTGGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.108

100

0.603
  pilU Vibrio cholerae strain A1552

58.378

100

0.584
  pilU Acinetobacter baylyi ADP1

60.563

95.946

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.436

97.838

0.405
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Acinetobacter baumannii strain A118

40.058

92.432

0.37
  pilT Acinetobacter nosocomialis M2

40.058

92.432

0.37
  pilT Acinetobacter baumannii D1279779

40.058

92.432

0.37
  pilT Legionella pneumophila strain Lp02

41.088

89.459

0.368
  pilT Legionella pneumophila strain ERS1305867

41.088

89.459

0.368
  pilT Acinetobacter baylyi ADP1

39.181

92.432

0.362