Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JZM60_RS12275 Genome accession   NZ_CP071382
Coordinates   2675242..2676339 (+) Length   365 a.a.
NCBI ID   WP_207162736.1    Uniprot ID   -
Organism   Geobacter benzoatilyticus strain Jerry-YX     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2670242..2681339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JZM60_RS12260 (JZM60_12260) - 2670704..2672563 (+) 1860 WP_207165589.1 DUF2723 domain-containing protein -
  JZM60_RS12265 (JZM60_12265) - 2672567..2673427 (+) 861 WP_207162734.1 shikimate dehydrogenase -
  JZM60_RS12270 (JZM60_12270) pilB 2673493..2675199 (+) 1707 WP_207162735.1 type IV-A pilus assembly ATPase PilB -
  JZM60_RS12275 (JZM60_12275) pilT 2675242..2676339 (+) 1098 WP_207162736.1 type IV pilus twitching motility protein PilT Machinery gene
  JZM60_RS12280 (JZM60_12280) - 2676370..2677587 (+) 1218 WP_207162737.1 type II secretion system F family protein -
  JZM60_RS12285 (JZM60_12285) - 2677597..2679240 (+) 1644 WP_207162738.1 two-component system sensor histidine kinase NtrB -
  JZM60_RS12290 (JZM60_12290) - 2679291..2680667 (+) 1377 WP_207162739.1 sigma-54-dependent transcriptional regulator -
  JZM60_RS12295 (JZM60_12295) pilV 2680885..2681112 (+) 228 WP_207162740.1 type IV pilin protein Machinery gene

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 40644.04 Da        Isoelectric Point: 8.1731

>NTDB_id=545126 JZM60_RS12275 WP_207162736.1 2675242..2676339(+) (pilT) [Geobacter benzoatilyticus strain Jerry-YX]
MANMHQLLTELVNRGGSDLHLTTNSPPQIRVDGKLIPLEMPVLNAVDTKQLCYSILTEQQKHKFEENNELDLSFGIKGLS
RFRGNVFVQRGAVAGVFRVIPYKILSFEELGLPPVVRELAEKPRGLVLVTGPTGSGKSTTLAAIIDKINTDRHEHIVTVE
DPIEYLHPHKSCVVNQREVGADTKSFKNALKYILRQDPDVVLVGELRDLETIEAALTLAETGHLCFATLHTNSAVQTINR
IVDVFPSYQQPQVRAQLSFVLEGVLSQTLLPRATGKGRVLAIEVMVPNPAIRNLIREDKIHQIYSQMQVGQEKFGMMTMN
QCLYGLLQKRHISMDVGMGRSPDPDELKQMLTSGVRPQGPRPPAR

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=545126 JZM60_RS12275 WP_207162736.1 2675242..2676339(+) (pilT) [Geobacter benzoatilyticus strain Jerry-YX]
ATGGCCAATATGCACCAGCTCCTTACGGAACTCGTTAATCGCGGCGGTTCCGATCTGCACCTGACCACCAATTCGCCCCC
GCAGATCCGGGTGGACGGGAAGCTCATTCCCCTGGAAATGCCGGTGCTGAACGCGGTGGACACGAAGCAGCTTTGCTACA
GCATTCTCACCGAGCAGCAGAAGCATAAGTTCGAGGAAAATAACGAGCTGGACCTCTCCTTCGGGATCAAGGGACTCTCC
CGTTTCCGGGGCAATGTTTTTGTCCAGCGGGGTGCCGTGGCCGGGGTTTTTCGGGTAATTCCGTACAAGATTCTATCCTT
CGAGGAGCTGGGCCTGCCACCGGTGGTGAGGGAACTGGCCGAGAAGCCCCGGGGGCTTGTCCTGGTAACCGGGCCCACCG
GCAGCGGTAAGTCGACAACCCTTGCTGCCATTATCGACAAGATAAACACCGACCGCCACGAGCATATCGTCACCGTCGAA
GATCCCATCGAGTATCTCCATCCCCACAAGAGTTGCGTTGTGAACCAGCGCGAGGTGGGGGCTGACACCAAGAGCTTCAA
GAACGCCCTCAAATACATACTCCGCCAGGACCCCGACGTGGTGCTGGTGGGCGAGCTGCGGGACCTGGAAACAATCGAGG
CGGCTCTGACGCTTGCCGAAACCGGGCACCTCTGCTTTGCCACCCTCCACACGAACTCGGCGGTCCAGACCATCAACCGG
ATTGTGGACGTGTTTCCGTCGTATCAGCAGCCTCAAGTCAGGGCTCAACTCTCATTCGTTCTCGAAGGGGTCTTGTCGCA
GACGCTTCTGCCGCGGGCAACCGGCAAGGGGAGGGTCCTCGCCATAGAGGTCATGGTGCCGAATCCGGCCATCCGCAACC
TCATCCGCGAGGACAAGATCCACCAGATATACTCGCAGATGCAGGTGGGGCAGGAAAAGTTCGGCATGATGACCATGAAC
CAGTGCCTTTATGGATTGCTGCAGAAACGGCATATCTCCATGGACGTTGGCATGGGAAGATCGCCCGACCCGGACGAGTT
GAAGCAGATGCTGACCAGCGGTGTGCGGCCTCAGGGACCGCGCCCTCCCGCAAGATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-255)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

56.193

90.685

0.51
  pilT Legionella pneumophila strain ERS1305867

56.193

90.685

0.51
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.586

95.342

0.501
  pilT Pseudomonas stutzeri DSM 10701

51.304

94.521

0.485
  pilT Acinetobacter baylyi ADP1

50.725

94.521

0.479
  pilT Pseudomonas aeruginosa PAK

50.725

94.521

0.479
  pilT Acinetobacter nosocomialis M2

52.266

90.685

0.474
  pilT Acinetobacter baumannii D1279779

52.266

90.685

0.474
  pilT Acinetobacter baumannii strain A118

52.266

90.685

0.474
  pilT Neisseria meningitidis 8013

48.802

91.507

0.447
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

89.589

0.444
  pilT Vibrio cholerae strain A1552

49.541

89.589

0.444
  pilT Neisseria gonorrhoeae MS11

48.048

91.233

0.438
  pilU Vibrio cholerae strain A1552

42.286

95.89

0.405
  pilU Pseudomonas stutzeri DSM 10701

41.243

96.986

0.4
  pilU Acinetobacter baylyi ADP1

39.481

95.068

0.375