Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FH974_RS02280 Genome accession   NZ_CP071325
Coordinates   460707..461744 (+) Length   345 a.a.
NCBI ID   WP_047887444.1    Uniprot ID   A0A0J1JS90
Organism   Photobacterium ganghwense strain C2.2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 455707..466744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FH974_RS02250 (FH974_02250) - 456781..457374 (-) 594 WP_047887450.1 XTP/dITP diphosphatase -
  FH974_RS02255 (FH974_02255) - 457467..457904 (-) 438 WP_047887449.1 DUF4426 domain-containing protein -
  FH974_RS02260 (FH974_02260) yggU 458038..458331 (-) 294 WP_047887448.1 DUF167 family protein YggU -
  FH974_RS02265 (FH974_02265) - 458328..458882 (-) 555 WP_047887447.1 YggT family protein -
  FH974_RS02270 (FH974_02270) proC 458896..459714 (-) 819 WP_047887446.1 pyrroline-5-carboxylate reductase -
  FH974_RS02275 (FH974_02275) - 459967..460683 (-) 717 WP_047887445.1 YggS family pyridoxal phosphate-dependent enzyme -
  FH974_RS02280 (FH974_02280) pilT 460707..461744 (+) 1038 WP_047887444.1 type IV pilus twitching motility protein PilT Machinery gene
  FH974_RS02285 (FH974_02285) pilU 461769..462875 (+) 1107 WP_047887443.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FH974_RS02290 (FH974_02290) ruvX 463380..463808 (-) 429 WP_047887442.1 Holliday junction resolvase RuvX -
  FH974_RS02295 (FH974_02295) - 463805..464371 (-) 567 WP_047887441.1 YqgE/AlgH family protein -
  FH974_RS02300 (FH974_02300) gshB 464505..465455 (-) 951 WP_207103469.1 glutathione synthase -
  FH974_RS02305 (FH974_02305) rsmE 465486..466217 (-) 732 WP_047887439.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FH974_RS02310 (FH974_02310) - 466216..466431 (+) 216 WP_047887438.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38366.75 Da        Isoelectric Point: 6.3384

>NTDB_id=545050 FH974_RS02280 WP_047887444.1 460707..461744(+) (pilT) [Photobacterium ganghwense strain C2.2]
MDITELLDFSVKHNASDLHLSAGVPPMIRVDGDVRKLSLPSLDHSEVHRLVFDIMNDAQRREFEEKLEVDFSFELPDVGR
FRVNAFHQSRGCAAVFRTIPVHVPTLESLKVPDIFYQIAQCQRGLVLVTGATGSGKSTTIAALVDYINENYNRHILTIED
PVEFVHHGKRCLINQREVHRDTHSFHNALRSSLREDPDVIVVGELRDQETISLALTAAETGHLVLGTLHTSSAAKTVDRI
IDVFPGNDKAMVRSMLSESLRAVVSQSLLKCTSGGRIAAHEVMMATPAIRNLIREDKVAQMYSMIQTGSSVGMQTMEQSV
RMLVAQGQVEAEEGRRVVDNAFAQF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=545050 FH974_RS02280 WP_047887444.1 460707..461744(+) (pilT) [Photobacterium ganghwense strain C2.2]
ATGGATATCACCGAACTACTGGACTTTAGTGTAAAGCATAATGCCTCGGATCTGCACCTCTCTGCGGGAGTTCCGCCGAT
GATTCGGGTAGATGGCGATGTTCGTAAACTGAGCTTGCCATCACTGGATCACAGTGAAGTACACCGCCTGGTGTTTGACA
TTATGAACGATGCCCAGCGGCGCGAGTTTGAAGAAAAGCTCGAAGTCGATTTCTCGTTCGAGCTGCCGGACGTCGGGCGG
TTTCGTGTGAATGCCTTTCATCAGTCACGGGGATGCGCAGCGGTTTTCCGAACCATCCCGGTGCATGTGCCGACACTGGA
ATCACTGAAAGTGCCGGACATATTCTATCAGATCGCCCAATGCCAGCGCGGGCTGGTGCTGGTTACCGGGGCAACCGGCT
CCGGTAAATCCACCACGATTGCTGCGCTGGTCGATTACATCAATGAAAACTATAACCGCCATATCCTGACGATTGAAGAT
CCGGTGGAGTTTGTTCACCACGGCAAACGTTGCCTGATTAACCAGCGTGAAGTACACCGCGATACCCACAGTTTCCACAA
TGCACTGCGCTCTTCCCTGCGTGAAGACCCGGATGTGATTGTGGTGGGGGAATTGCGTGATCAGGAAACCATCAGTCTGG
CGCTGACCGCAGCAGAAACCGGCCACCTTGTGCTGGGCACTCTGCACACCAGCTCGGCGGCCAAAACCGTCGATCGGATC
ATTGATGTCTTCCCGGGGAACGACAAGGCGATGGTGCGTTCTATGTTGTCTGAGTCGCTGCGGGCGGTGGTATCGCAGAG
CCTGCTCAAATGTACCAGCGGAGGCCGAATTGCGGCGCATGAAGTCATGATGGCGACCCCGGCTATCCGTAACCTGATTC
GTGAAGACAAAGTGGCCCAGATGTATTCCATGATCCAGACCGGCTCATCCGTCGGGATGCAGACCATGGAGCAAAGTGTC
CGGATGCTGGTGGCTCAGGGGCAGGTTGAGGCGGAAGAAGGACGCCGGGTCGTCGACAACGCGTTTGCGCAGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0J1JS90

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

77.477

96.522

0.748
  pilT Vibrio cholerae strain A1552

77.477

96.522

0.748
  pilT Acinetobacter baumannii D1279779

70.149

97.101

0.681
  pilT Acinetobacter baumannii strain A118

70.149

97.101

0.681
  pilT Acinetobacter nosocomialis M2

69.851

97.101

0.678
  pilT Acinetobacter baylyi ADP1

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

68.358

97.101

0.664
  pilT Pseudomonas stutzeri DSM 10701

67.761

97.101

0.658
  pilT Legionella pneumophila strain Lp02

67.584

94.783

0.641
  pilT Legionella pneumophila strain ERS1305867

67.584

94.783

0.641
  pilT Neisseria meningitidis 8013

64.035

99.13

0.635
  pilT Neisseria gonorrhoeae MS11

63.743

99.13

0.632
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.974

98.841

0.484
  pilU Vibrio cholerae strain A1552

41.228

99.13

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.916

96.812

0.406
  pilU Acinetobacter baylyi ADP1

39.441

93.333

0.368