Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FH974_RS02285 Genome accession   NZ_CP071325
Coordinates   461769..462875 (+) Length   368 a.a.
NCBI ID   WP_047887443.1    Uniprot ID   A0A0J1GZT4
Organism   Photobacterium ganghwense strain C2.2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 456769..467875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FH974_RS02250 (FH974_02250) - 456781..457374 (-) 594 WP_047887450.1 XTP/dITP diphosphatase -
  FH974_RS02255 (FH974_02255) - 457467..457904 (-) 438 WP_047887449.1 DUF4426 domain-containing protein -
  FH974_RS02260 (FH974_02260) yggU 458038..458331 (-) 294 WP_047887448.1 DUF167 family protein YggU -
  FH974_RS02265 (FH974_02265) - 458328..458882 (-) 555 WP_047887447.1 YggT family protein -
  FH974_RS02270 (FH974_02270) proC 458896..459714 (-) 819 WP_047887446.1 pyrroline-5-carboxylate reductase -
  FH974_RS02275 (FH974_02275) - 459967..460683 (-) 717 WP_047887445.1 YggS family pyridoxal phosphate-dependent enzyme -
  FH974_RS02280 (FH974_02280) pilT 460707..461744 (+) 1038 WP_047887444.1 type IV pilus twitching motility protein PilT Machinery gene
  FH974_RS02285 (FH974_02285) pilU 461769..462875 (+) 1107 WP_047887443.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FH974_RS02290 (FH974_02290) ruvX 463380..463808 (-) 429 WP_047887442.1 Holliday junction resolvase RuvX -
  FH974_RS02295 (FH974_02295) - 463805..464371 (-) 567 WP_047887441.1 YqgE/AlgH family protein -
  FH974_RS02300 (FH974_02300) gshB 464505..465455 (-) 951 WP_207103469.1 glutathione synthase -
  FH974_RS02305 (FH974_02305) rsmE 465486..466217 (-) 732 WP_047887439.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FH974_RS02310 (FH974_02310) - 466216..466431 (+) 216 WP_047887438.1 hypothetical protein -
  FH974_RS02315 (FH974_02315) endA 466459..467163 (-) 705 WP_207103470.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41183.25 Da        Isoelectric Point: 6.1452

>NTDB_id=545051 FH974_RS02285 WP_047887443.1 461769..462875(+) (pilU) [Photobacterium ganghwense strain C2.2]
MTLQDALNEMVSSKASDLYITVEAPCLLKIDGNLHPLGQALDRPAIDRMFSEAMDDALYQEFCATKEANFALVRGEYRFR
VSAFWQRELPGMVIRRIETDIPDIFALNLPMDMQKMALAKRGLVLVVGATGSGKSTSLAAMTGYRNANRSGHILTVEDPI
EFVHKHNKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTRETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERRDQFLFDLSLNLKCILAQQLIPDAHGMGRHGAFELLINTPRVADLIRRGELHEIKDTMAKSAQSGMMTFDQSLY
DLYSAGKITEQDALHHADSANDLRLMIKVGNKHHQANSSLDGVTIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=545051 FH974_RS02285 WP_047887443.1 461769..462875(+) (pilU) [Photobacterium ganghwense strain C2.2]
ATGACATTACAGGATGCGCTGAATGAAATGGTCAGTAGCAAAGCCTCCGATTTGTACATCACGGTGGAAGCGCCATGTCT
GCTGAAAATCGACGGTAATCTGCACCCGTTGGGGCAGGCGCTGGATCGTCCGGCGATTGACCGGATGTTCAGCGAAGCCA
TGGACGACGCGTTGTATCAGGAGTTTTGTGCCACTAAAGAAGCAAACTTTGCGCTGGTACGCGGCGAGTACCGGTTCCGG
GTCAGTGCCTTCTGGCAGCGGGAGCTGCCGGGCATGGTGATCCGGCGGATTGAAACCGATATCCCGGACATTTTTGCCCT
TAACTTGCCGATGGACATGCAGAAGATGGCACTGGCGAAGCGGGGGCTGGTGCTGGTGGTCGGGGCGACCGGCTCGGGCA
AATCCACGTCACTGGCGGCTATGACCGGCTACCGTAATGCCAACCGTAGCGGTCACATTCTGACGGTGGAAGATCCGATT
GAGTTCGTGCACAAGCATAATAAGTGCATCGTGACCCAGCGCGAAGTCGGCTTGGATACGGAGAGCTACGAGGTGGCGCT
CAAGAACTCGCTGCGCCAGGCTCCTGACATGATTCTGATTGGCGAAATCCGGACCCGGGAAACCATGGAATATGCCATGA
ACTTTGCCGAAACCGGGCACCTGTGTATGGCGACCTTGCATGCCAATAATGCCAACCAGGCGTTGGAGCGTATTCTCCAT
CTGGTTCCGAAAGAGCGGCGGGATCAGTTTTTGTTCGATTTATCACTGAACCTGAAATGTATTCTGGCTCAGCAGCTGAT
CCCGGATGCCCACGGTATGGGACGGCACGGTGCCTTTGAGCTGTTGATCAACACGCCACGGGTTGCGGATCTGATCCGTC
GGGGTGAGCTGCACGAAATCAAGGACACCATGGCGAAATCAGCCCAGTCGGGGATGATGACGTTTGATCAGTCTCTGTAT
GACCTCTATAGCGCCGGCAAGATCACGGAGCAGGACGCTTTGCACCATGCCGATTCGGCCAACGATCTGCGACTGATGAT
TAAAGTCGGCAACAAACATCATCAGGCAAACAGCAGCTTGGATGGGGTCACGATCGATATGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(109-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0J1GZT4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

69.837

100

0.698
  pilU Pseudomonas stutzeri DSM 10701

58.221

100

0.587
  pilU Acinetobacter baylyi ADP1

54.795

99.185

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.653

91.576

0.372