Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M5M_RS11155 Genome accession   NC_018868
Coordinates   2518443..2519477 (-) Length   344 a.a.
NCBI ID   WP_016389360.1    Uniprot ID   K4KMH8
Organism   Simiduia agarivorans SA1 = DSM 21679     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2513443..2524477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5M_RS11125 (M5M_11260) pilG 2513632..2514027 (-) 396 WP_015047593.1 twitching motility response regulator PilG Regulator
  M5M_RS11130 (M5M_11265) gshB 2514357..2515313 (+) 957 WP_015047594.1 glutathione synthase -
  M5M_RS11135 (M5M_11270) - 2515310..2516206 (+) 897 WP_015047595.1 energy transducer TonB -
  M5M_RS11140 (M5M_11275) - 2516267..2516851 (+) 585 WP_015047596.1 YqgE/AlgH family protein -
  M5M_RS11145 (M5M_11280) ruvX 2516835..2517278 (+) 444 WP_015047597.1 Holliday junction resolvase RuvX -
  M5M_RS11150 (M5M_11285) pilU 2517288..2518430 (-) 1143 WP_015047598.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M5M_RS11155 (M5M_11290) pilT 2518443..2519477 (-) 1035 WP_016389360.1 type IV pilus twitching motility protein PilT Machinery gene
  M5M_RS11160 (M5M_11295) - 2519568..2520254 (+) 687 WP_016389361.1 YggS family pyridoxal phosphate-dependent enzyme -
  M5M_RS11165 (M5M_11305) proC 2520310..2521128 (+) 819 WP_015047602.1 pyrroline-5-carboxylate reductase -
  M5M_RS11170 (M5M_11310) - 2521130..2521720 (+) 591 WP_015047603.1 YggT family protein -
  M5M_RS11175 (M5M_11315) - 2521733..2522698 (-) 966 WP_015047604.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38218.97 Da        Isoelectric Point: 7.0158

>NTDB_id=54088 M5M_RS11155 WP_016389360.1 2518443..2519477(-) (pilT) [Simiduia agarivorans SA1 = DSM 21679]
MDITELLAFSAKQGASDLHLSSGLPPMIRVDGDVRRINLPPMQHKQVHSLIYEIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEELGMGQVFRDICNTPRGICLVTGPTGSGKSTTLAAMVDYINDNKYQHILTIED
PIEFVHESKKCLINQREVHRDTHGFSAALRSALREDPDIILVGEMRDLETIKLALEAAETGHLVFGTLHTQSAAKTIDRI
IDVFPAAEKAMVRSMLSESLQAVISQTLMKKNGGGRVAAHEIMRGTPAIRNLIREDKVAQMYSAIQTGASVGMQTMDQCL
ADLVARRVIARDVAREKAKIPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=54088 M5M_RS11155 WP_016389360.1 2518443..2519477(-) (pilT) [Simiduia agarivorans SA1 = DSM 21679]
ATGGATATTACCGAATTATTGGCATTCTCCGCGAAACAAGGGGCGTCTGACTTGCATCTGTCTTCCGGCCTGCCGCCCAT
GATACGCGTAGACGGCGATGTGCGCCGGATTAATTTGCCGCCCATGCAGCACAAGCAGGTTCACAGTCTCATTTACGAGA
TCATGAACGACAAGCAGCGCAAAGATTACGAAGAATTCCTCGAAACTGACTTCTCTTTCGAAGTGCCGGGTGTGGCGCGC
TTCCGGGTTAACGCCTTTAACCAGAACCGCGGAGCCGGTGCGGTATTCCGGACCATTCCCTCAAAAGTTCTGACCATGGA
AGAGCTGGGCATGGGTCAGGTGTTCCGCGATATCTGTAATACCCCGCGCGGCATTTGTCTGGTGACCGGGCCCACGGGTT
CGGGTAAGTCCACGACGCTGGCGGCCATGGTGGATTACATTAACGACAACAAATACCAGCACATCCTCACCATTGAGGAC
CCGATCGAATTCGTGCACGAATCAAAAAAATGCCTGATTAACCAGCGGGAAGTGCATCGCGATACCCACGGCTTCTCCGC
CGCCTTGCGTTCGGCATTGCGGGAAGACCCAGACATTATTCTGGTGGGTGAGATGCGCGATCTGGAAACCATCAAGCTGG
CGCTGGAAGCGGCCGAAACCGGTCACCTGGTGTTCGGCACCCTGCACACCCAGTCGGCGGCGAAAACCATCGACCGTATT
ATCGACGTATTCCCGGCCGCAGAAAAAGCCATGGTGCGTTCCATGTTGTCGGAATCATTGCAGGCGGTGATTTCACAGAC
CCTGATGAAGAAAAACGGCGGTGGTCGGGTGGCGGCGCACGAAATCATGCGCGGTACGCCGGCGATCCGTAACCTGATCC
GTGAAGATAAAGTGGCGCAGATGTACTCGGCCATTCAGACCGGTGCTTCGGTAGGCATGCAGACTATGGATCAGTGCCTG
GCCGACCTGGTGGCCCGACGGGTGATTGCGCGCGATGTGGCGCGCGAAAAAGCCAAAATTCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K4KMH8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

86.919

100

0.869
  pilT Pseudomonas stutzeri DSM 10701

86.628

100

0.866
  pilT Acinetobacter baumannii D1279779

83.43

100

0.834
  pilT Acinetobacter baumannii strain A118

83.43

100

0.834
  pilT Acinetobacter nosocomialis M2

83.14

100

0.831
  pilT Acinetobacter baylyi ADP1

80.233

100

0.802
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.64

0.68
  pilT Vibrio cholerae strain A1552

71.125

95.64

0.68
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39
  pilU Pseudomonas stutzeri DSM 10701

40.741

94.186

0.384
  pilU Vibrio cholerae strain A1552

40.308

94.477

0.381

Multiple sequence alignment