Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M5M_RS11150 Genome accession   NC_018868
Coordinates   2517288..2518430 (-) Length   380 a.a.
NCBI ID   WP_015047598.1    Uniprot ID   K4KJU9
Organism   Simiduia agarivorans SA1 = DSM 21679     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2512288..2523430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5M_RS11115 (M5M_11250) - 2512615..2513169 (-) 555 WP_015047591.1 chemotaxis protein CheW -
  M5M_RS11120 (M5M_11255) pilH 2513197..2513559 (-) 363 WP_015047592.1 twitching motility response regulator PilH -
  M5M_RS11125 (M5M_11260) pilG 2513632..2514027 (-) 396 WP_015047593.1 twitching motility response regulator PilG Regulator
  M5M_RS11130 (M5M_11265) gshB 2514357..2515313 (+) 957 WP_015047594.1 glutathione synthase -
  M5M_RS11135 (M5M_11270) - 2515310..2516206 (+) 897 WP_015047595.1 energy transducer TonB -
  M5M_RS11140 (M5M_11275) - 2516267..2516851 (+) 585 WP_015047596.1 YqgE/AlgH family protein -
  M5M_RS11145 (M5M_11280) ruvX 2516835..2517278 (+) 444 WP_015047597.1 Holliday junction resolvase RuvX -
  M5M_RS11150 (M5M_11285) pilU 2517288..2518430 (-) 1143 WP_015047598.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M5M_RS11155 (M5M_11290) pilT 2518443..2519477 (-) 1035 WP_016389360.1 type IV pilus twitching motility protein PilT Machinery gene
  M5M_RS11160 (M5M_11295) - 2519568..2520254 (+) 687 WP_016389361.1 YggS family pyridoxal phosphate-dependent enzyme -
  M5M_RS11165 (M5M_11305) proC 2520310..2521128 (+) 819 WP_015047602.1 pyrroline-5-carboxylate reductase -
  M5M_RS11170 (M5M_11310) - 2521130..2521720 (+) 591 WP_015047603.1 YggT family protein -
  M5M_RS11175 (M5M_11315) - 2521733..2522698 (-) 966 WP_015047604.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42300.62 Da        Isoelectric Point: 6.1366

>NTDB_id=54087 M5M_RS11150 WP_015047598.1 2517288..2518430(-) (pilU) [Simiduia agarivorans SA1 = DSM 21679]
MDFDRLLTLMVEKGASDLFITAGVPPSVKLHGKVVPVTTTPLSPEKSREVVLSVMNEKQRTEFLETKELNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPKVDDLGLPEIIKELAMTKRGLIIFVGATGTGKSTSLAAMIGHRNRNSKGHIITIED
PIEFIHQHQGCIVTQREVGIDTDSFEIALKNTLRQAPDVILIGEVRSRETMEHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHSQLWMDLALNLRGIIAQQLIPTPDGNGRRACLEVLLNTPLAQDLIRKGAVHELKPLMKKSTELGMQTFDQA
LYELYDSGEITYEDALSYADSPNDLRLLIKLGSETDADYLINAADELSIQKEENSGNKMY

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=54087 M5M_RS11150 WP_015047598.1 2517288..2518430(-) (pilU) [Simiduia agarivorans SA1 = DSM 21679]
ATGGATTTTGATCGGCTTCTGACGCTGATGGTTGAAAAAGGCGCCTCGGATTTGTTTATTACTGCCGGCGTGCCGCCCTC
GGTTAAATTGCACGGCAAAGTAGTGCCGGTGACCACCACACCCCTGTCGCCCGAAAAATCGCGCGAAGTGGTGTTATCGG
TGATGAATGAAAAGCAGCGCACCGAATTTCTTGAAACCAAGGAACTCAACTTTGCGATTTCCGCGCGCGGTATCGGCCGT
TTCCGTGTGAGTGCCTTTTACCAACGCAACCTGGCGGGCATGGTGTTGCGTCGGATTGAAACCAATATTCCGAAAGTGGA
TGATCTGGGTTTGCCGGAAATCATCAAAGAGCTGGCGATGACCAAGCGCGGCCTGATTATCTTTGTGGGCGCCACCGGTA
CCGGTAAGTCCACCTCGCTGGCGGCGATGATCGGACACCGCAACCGCAACTCCAAAGGCCACATCATCACCATCGAAGAC
CCGATCGAATTCATTCACCAGCACCAGGGTTGCATCGTGACCCAGCGCGAGGTGGGGATTGATACGGATTCGTTTGAGAT
CGCGCTGAAAAATACGCTGCGTCAGGCGCCCGACGTGATTCTGATTGGTGAGGTGCGTTCGCGCGAAACCATGGAGCACG
CCATTGCGTTCGCCGAAACCGGCCACCTGTGTTTGTGTACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCCATTTCTTCCCGGCCGATCGCCACAGTCAGTTGTGGATGGACCTGGCCCTGAACCTGCGCGGCATTATCGCCCAGCA
ATTGATTCCCACACCCGATGGCAATGGCCGTCGCGCGTGTCTGGAAGTGTTGTTGAATACGCCGCTGGCGCAGGATCTGA
TTCGCAAGGGCGCGGTGCATGAACTGAAACCGCTGATGAAAAAATCCACCGAGCTGGGCATGCAGACTTTCGACCAGGCG
CTGTACGAGCTTTATGACAGCGGCGAAATCACTTACGAAGATGCGCTGTCTTACGCGGATTCACCCAACGACCTGCGCCT
GCTGATCAAGCTGGGCTCGGAAACCGATGCGGATTATCTGATTAATGCGGCCGATGAATTGTCGATTCAGAAAGAAGAGA
ATTCCGGCAATAAGATGTATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K4KJU9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

75.789

100

0.758
  pilU Acinetobacter baylyi ADP1

67.787

93.947

0.637
  pilU Vibrio cholerae strain A1552

56

92.105

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.678

91.579

0.4
  pilT Legionella pneumophila strain ERS1305867

41.493

88.158

0.366
  pilT Legionella pneumophila strain Lp02

41.493

88.158

0.366
  pilT Pseudomonas aeruginosa PAK

40

90.789

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.653

88.684

0.361
  pilT Vibrio cholerae strain A1552

40.653

88.684

0.361

Multiple sequence alignment