Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JWZ98_RS21460 Genome accession   NZ_CP070494
Coordinates   4677362..4678399 (-) Length   345 a.a.
NCBI ID   WP_101053853.1    Uniprot ID   A0A2N0V4Z8
Organism   Methylomonas sp. EFPC1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4672362..4683399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JWZ98_RS21440 (JWZ98_21440) - 4673303..4673839 (+) 537 WP_205451823.1 transposase -
  JWZ98_RS21445 (JWZ98_21445) - 4673960..4674514 (+) 555 WP_129871319.1 hypothetical protein -
  JWZ98_RS21450 (JWZ98_21450) mltB 4675015..4676196 (-) 1182 WP_205451825.1 lytic murein transglycosylase B -
  JWZ98_RS21455 (JWZ98_21455) pilU 4676214..4677347 (-) 1134 WP_171696916.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JWZ98_RS21460 (JWZ98_21460) pilT 4677362..4678399 (-) 1038 WP_101053853.1 type IV pilus twitching motility protein PilT Machinery gene
  JWZ98_RS21465 (JWZ98_21465) - 4678516..4678857 (-) 342 WP_033158299.1 TraR/DksA family transcriptional regulator -
  JWZ98_RS21470 (JWZ98_21470) - 4679003..4679908 (+) 906 WP_205451826.1 AAA family ATPase -
  JWZ98_RS21475 (JWZ98_21475) - 4679905..4680855 (+) 951 WP_205451828.1 DUF58 domain-containing protein -
  JWZ98_RS21480 (JWZ98_21480) - 4680828..4682807 (+) 1980 WP_240348129.1 DUF3488 and DUF4129 domain-containing transglutaminase family protein -
  JWZ98_RS21485 (JWZ98_21485) - 4682917..4683180 (-) 264 WP_033158303.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38214.12 Da        Isoelectric Point: 7.0291

>NTDB_id=540211 JWZ98_RS21460 WP_101053853.1 4677362..4678399(-) (pilT) [Methylomonas sp. EFPC1]
MDIAELLTFSVKNKASDLHLSAGLPPMIRVDGDIRRINIPSLNHKEVHALIYDIMNDKQRRDYEEFLETDFSFALPGVAR
FRVNAFNQDRGAGAVFRTIPSKVLTLEDLDAPKFFAELCTKPRGLILVTGPTGSGKSTTLAAMVNHINTNDYAHILTVED
PIEFVHESQKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMIRAMLSESLQAVISQTLLKKVGGGRIAAHEIMVGTPAIRNLIREAKVAQMYSAIQTGRKDGMQTLDQNL
KELVDKGLITAKGAMVKAVNKDMFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=540211 JWZ98_RS21460 WP_101053853.1 4677362..4678399(-) (pilT) [Methylomonas sp. EFPC1]
ATGGATATCGCCGAACTCTTGACCTTTTCCGTCAAAAACAAAGCCTCCGACTTGCATCTTTCCGCCGGCCTGCCGCCGAT
GATTCGGGTGGACGGCGACATCCGCCGCATCAATATCCCGTCCTTGAATCACAAAGAAGTCCACGCGCTGATTTACGATA
TCATGAACGACAAGCAGCGCCGCGATTACGAAGAGTTTCTGGAAACCGACTTCTCGTTCGCGCTGCCCGGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAATCAGGATAGAGGCGCCGGCGCGGTATTCCGGACCATTCCGTCCAAGGTGTTAACCCTGGA
AGACCTGGACGCGCCGAAATTTTTCGCGGAACTGTGTACCAAGCCGCGCGGCCTGATTCTGGTGACCGGTCCGACCGGTT
CCGGTAAATCCACCACGCTGGCGGCGATGGTGAATCACATTAATACCAACGATTACGCACATATCCTGACCGTCGAAGAC
CCTATCGAGTTTGTCCACGAAAGCCAGAAATGCTTGATCAACCAGCGCGAAGTACATCGCGATACCTTAGGTTTCAACGA
AGCCTTGCGCTCGGCATTACGGGAAGACCCGGACATCATTCTGGTGGGTGAGATGCGGGATTTGGAAACCATCCGCCTGG
CATTGACCGCCGCGGAAACCGGTCACTTGGTATTCGGCACGCTGCATACCACCTCGGCCGCCAAAACCATAGACCGGATT
ATCGACGTATTCCCTGCGGCGGAAAAAGACATGATCCGGGCGATGTTGTCCGAATCTTTGCAGGCGGTTATCTCGCAAAC
GCTATTGAAAAAAGTCGGCGGCGGCCGGATTGCCGCGCACGAAATCATGGTCGGTACGCCGGCCATCCGTAACCTGATTC
GCGAAGCCAAGGTAGCGCAGATGTATTCCGCGATCCAAACCGGCCGCAAGGACGGCATGCAGACCCTGGACCAAAATCTG
AAGGAGTTGGTCGATAAAGGCCTAATTACTGCGAAAGGCGCGATGGTTAAAGCTGTCAACAAAGACATGTTCCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N0V4Z8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

80.233

99.71

0.8
  pilT Acinetobacter baumannii strain A118

80.233

99.71

0.8
  pilT Acinetobacter nosocomialis M2

80.233

99.71

0.8
  pilT Acinetobacter baylyi ADP1

79.36

99.71

0.791
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Pseudomonas aeruginosa PAK

80.473

97.971

0.788
  pilT Legionella pneumophila strain Lp02

72.965

99.71

0.728
  pilT Legionella pneumophila strain ERS1305867

72.965

99.71

0.728
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.03

97.681

0.684
  pilT Vibrio cholerae strain A1552

70.03

97.681

0.684
  pilT Neisseria meningitidis 8013

66.181

99.42

0.658
  pilT Neisseria gonorrhoeae MS11

65.889

99.42

0.655
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.507
  pilU Acinetobacter baylyi ADP1

40.634

100

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.173

100

0.403
  pilU Vibrio cholerae strain A1552

40.118

98.261

0.394